fluorescence-activated cell sorting
Spatial dissection of the Arabidopsis thaliana transcriptional response to downy mildew using fluorescence activated cell sorting
... use Fluorescence Activated Cell Sorting (FACS) as it is a rapid way of isolating a large number of cells for gene expression analysis (Karve and Iyer-Pascuzzi, ...allows sorting of ...
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The use of fluorescence activated cell sorting in studying plant development and environmental responses
... 1. Fluorescence-Activated Cell Sorting (FACS) ...conferring cell- or tissue-specific expression are chosen for an experi- ment; for instance, Medicago truncatula roots expressing GFP ...
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The use of fluorescence activated cell sorting in studying plant development and environmental responses
... 1. Fluorescence-Activated Cell Sorting (FACS) ...conferring cell- or tissue-specific expression are chosen for an experi- ment; for instance, Medicago truncatula roots expressing GFP ...
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Cell specific analysis of Arabidopsis leaves using fluorescence activated cell sorting
... Grønlund, Jesper T., Eyres, Alison, Kumar, S., Buchanan-Wollaston, Vicky and Gifford, M. L.. (2012) Cell Specific Analysis of Arabidopsis Leaves Using Fluorescence Activated Cell ...
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The proliferation and differentiation of osteoblasts in co-culture with human umbilical vein endothelial cells: An improved analysis using fluorescence-activated cell sorting
... inhibits cell proliferation and promotes cell ...higher cell density. After 120 h, the total cell number of MG- 63 cells in the single culture was about twice as high as that in the ...the ...
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Maintaining RNA Integrity for Transcriptomic Profiling of Ex Vivo Cultured Limbal Epithelial Stem Cells after Fluorescence-Activated Cell Sorting (FACS)
... the sorting of heterogeneous cell populations, we rely on both extracellular and intracellular markers, and therefore need to fix and permeabilize the cells prior to staining and ...
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Analysis of differential gene expression in colorectal cancer and stroma using fluorescence-activated cell sorting purification
... Nevertheless, the available data allows one to draw some interesting comparisons. Unlike colorectal cancer, somatic mutation in breast cancer appears largely TGF-β1 independent. Only 3 of the 24 CAN genes identified in ...
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Functional characterisation of human pulmonary monocyte-like cells in lipopolysaccharide-mediated acute lung inflammation
... Figure 2 Fluorescence activated cell sorting of cells in BAL fluid following LPS inhalation. Cells were identified in BAL fluid based upon their position on flow cytometry dot plots of size ...
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A novel anti-EMMPRIN function-blocking antibody reduces T cell proliferation and neurotoxicity: relevance to multiple sclerosis
... Using fluorescence-activated cell sorting (FACS) analysis (A), mouse astrocytes identified by glial fibrillary acidic protein (GFAP) were examined for extracellular matrix metalloproteinase ...
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Capturing Alzheimer's disease genomes with induced pluripotent stem cells: prospects and challenges
... identified cell surface molecular signatures that allow the purification of neural precursor cells (NPCs) and neurons from differentiated hESCs by fluorescenceactivated cell sorting ...stem ...
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Asymmetric triplex metallohelices with high and selective activity against cancer cells
... In triplicate, and against untreated control cells, drug-treated HCT116 wild type p53 cells were washed twice in incubation buffer (PBS containing BSA) then re-suspended. Primary rabbit anti- human phosphor Histone H2AX ...
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Combination of BCL11A siRNA with vincristine increases the apoptosis of SUDHL6 cells
... a fluorescence microscope (Leica, ...to fluorescence-activated cell sorting (FACS) analysis (BD FACS Calibur, Franklin Lakes, New Jersey, ...
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Regulatory roles of interferon-inducible protein 204 on differentiation and vasculogenic activity of endothelial progenitor cells
... of cell growth and ...Using fluorescence-activated cell sorting, immunocytochemistry, and quantitative PCR, endothelial markers including CD31, VE-cadherin, and vWF were detected in the ...
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Monocyte galactose/N-acetylgalactosamine-specific C-type lectin receptor stimulant immunotherapy of an experimental glioma. Part II: combination with external radiation improves survival
... Methods: C57BL/6 mice underwent stereotactic intracranial implantation of GL261 glioma cells. Animals were grouped based on randomized tumor size by magnetic resonance imaging on day seven. One group that received ...
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Spontaneous opticospinal encephalomyelitis in a double transgenic mouse model of autoimmune T cell/B cell cooperation
... The majority of these opticospinal EAE OSE mice had a paralytic disease caused by inflammatory demyelinating Nonstandard abbreviations used: FACS, fluorescence-activated cell sorting; Ig[r] ...
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Biological Signature Characteristics of Primary Isolates from Human Immunodeficiency Virus Type 1 Group O in Ex Vivo Human Tonsil Histocultures
... proteins. Fluorescence-activated cell sorting (FACS) analysis was used to measure the ratio of CD4 T cells to CD8 T cells in infected and uninfected cultures, ...
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An efficient Trojan delivery of tetrandrine by poly(N-vinylpyrrolidone)-block-poly(ε-caprolactone) (PVP-b-PCL) nanoparticles shows enhanced apoptotic induction of lung cancer cells and inhibition of its migration and invasion
... including fluorescence activated cell sorting and Western blotting indicated that this Trojan strategy of delivering Tet in PVP-b-PCL nanoparticles via endocytosis leads to enhanced induction ...
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Asymmetric triplex metallohelices with high and selective activity against cancer cells
... In triplicate, and against untreated control cells, drug-treated HCT116 wild type p53 cells were washed twice in incubation buffer (PBS containing BSA) then re-suspended. Primary rabbit anti- human phosphor Histone H2AX ...
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CD15 Expression Does Not Identify a Phenotypically or Genetically Distinct Glioblastoma Population.
... CD152 cell popu- lations exposed to the same environmental conditions in vivo to Figure ...by fluorescence-activated cell sorting (FACs) established a phenotypic equilibrium that ...
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Clonally expanded human airway smooth muscle cells exhibit morphological and functional heterogeneity
... single cell sorting in 96 well culture plates using Coulter Epics Altra Hypersort fluorescence-activated cell sorter ...the cell sort gating criteria ensured that only cells with ...
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