Fluorescence polarization assay
Determining a Diagnostic Cut Off on Fluorescence Polarization Assay (FPA) for Bovine Brucellosis in Carchi, Ecuador
... through fluorescence polarization assay (FPA) in Carchi—Ecuador was ...Fluoresce Polarization Assay (FPA) and competitive enzyme-linked inmunosorbent assay (cELISA) as ...
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Use of Recombinant Flagellin Protein as a Tracer Antigen in a Fluorescence Polarization Assay for Diagnosis of Leptospirosis
... The objective of the present study was to investigate the usefulness of a recombinant flagellar protein, FlaB, of Leptospira interrogans serovar pomona in the serodiagnosis of leptospirosis by the fluorescence ...
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Use of the Brucella melitensis Native Hapten To Diagnose Brucellosis in Goats by a Rapid, Simple, and Specific Fluorescence Polarization Assay
... the fluorescence polarization assay (FPA) using the recently described Brucella melitensis native hapten and the Brucella abortus O-polysaccharide tracer was evaluated and compared with those of The ...
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Application of the Fluorescence Polarization Assay for Detection of Caprine Antibodies to Brucella melitensis in Areas of High Prevalence and Widespread Vaccination
... The World Organization for Animal Health (OIE) (15)- approved tests for diagnosis of brucellosis in cattle are the buffered Brucella antigen (BBA) tests (Rose Bengal test [RBT] and buffered plate agglutination test ...
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Fluorescence Polarization Assay for Detection of Brucella abortus Antibodies in Bulk Tank Bovine Milk Samples
... inexpensive fluorescence polarization assay for the detection of antibodies to Brucella abortus in bulk tank milk samples at the farm level or at dairies with a sensitivity and specificity of 100 and ...
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Rapid screening and identification of dominant B cell epitopes of HBV surface antigen by quantum dot based fluorescence polarization assay
... antigenicity of this peptide can be easily determined. For instance, when the QD-labeled peptides are mixed with the standard antibody in solution, if the peptides have antigenicity, they can bind with antibodies ...
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Detection of HBV Genotypes of Tumor Tissues and Serum by A Fluorescence Polarization Assay in North Western China's Hepatocellular Carcinoma Patients
... FP assay in serum and matched primary tissue samples of the HCC patients in north-western ...FP assay was sim- ple and cost-effective because HBV genotypes A-D could be detected by a single PCR reaction ...
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Evaluation of quantitative assays for the identification of direct signal transducer and activator of transcription 3 (STAT3) inhibitors
... vitro fluorescence polarization assay, using recombinant STAT3 protein, has successfully identified compounds that target the SH2 domain; however, no assay has been reported to identify ...
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Seroprevalence of Bovine Brucellosis in Albania Dairy Cattle. Impact of Test and Slaughter Strategy in Herd Prevalence
... (fluorescence polarization assay) must be carried out in conjunction with MRT and RBT in order to confirm the brucellosis status [4, 5, 6, 7, ...
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Modification of the Mycobacterium bovis extracellular protein MPB70 with fluorescein for rapid detection of specific serum antibodies by fluorescence polarization.
... observed polarization is dependent solely on ~ ...constant, polarization is dependent on the molecular volume, that is, the size of a fluorescent ...High polarization values indicate that the ...
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Fluorescence of Senna simea Lam leaf extracts: A possible interference in a fluorescence based assay
... reuptake assay kit. After measuring the intracellular fluorescence, we calculated RFU area below curve reflecting the activity of dopamine transporter (DAT), norepinephrine transporter (NET), serotonin ...
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Screening for novel inhibitors of phospho MurNAc pentapeptide translocase MraY
... in fluorescence intensity, indicating the formation of dansyl-labelled lipid ...in fluorescence over 5-15 minutes so that the reaction kinetics can be studied, which meant ~6 mg total protein/ml stock ...
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CD18/ICAM 1 dependent oxidative NF kappaB activation leading to nitric oxide production in rat Kupffer cells cocultured with syngeneic hepatoma cells
... The fluorescence DNA-protein binding assay used in this study is based on the nature of ...FITC fluorescence in nuclei under the laser scanning confocal ...this assay clearly revealed that ...
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Assessment of membrane fluidity in individual yeast cells by Laurdan generalised polarisation and multi-photon scanning fluorescence microscopy
... Generalized Polarization (GP), derived from fluorescence intensities at critical wavelengths, can be considered as an index of membrane fluidity ...the Polarization parameter, exchanging values at ...
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Comparison of enzyme linked immunosorbent assay with enzyme linked fluorescence assay with automated readers for detection of rubella virus antibody and herpes simplex virus
... The enzyme-linked immunosorbent assay ELISA was compared with the enzyme-linked fluorescence assay ELFA for the detection of rubella antibody and herpes simplex virus antigen.. Test para[r] ...
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R4 and R12 subfamily RGS proteins : structures, functions, and emerging chemical biology
... with assay medium (DMEM with 10% FBS (without phenol), 15 mM HEPES pH ...forskolin-containing assay medium) was added to each well and allowed to incubate for 10 minutes before GloSensor emission was read ...
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Enzyme linked fluorescence assay: Ultrasensitive solid phase assay for detection of human rotavirus
... In addition, the sensitivity of the rotaviral detection systems was established by testing logio dilutions in quadruplicate of a standard filtrate prepared from the stool of a gnotobioti[r] ...
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A poxvirus-encoded uracil DNA glycosylase is essential for virus viability.
... Ethidium bromide fluorescence assay of the refolded SFV uracil DNA glycosylase using uracil-containing plasmid DNA as a substrate.. Following treatment with the enzyme, the fluorescence [r] ...
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Effects of extremely low frequency electromagnetic fields on human chromosomes : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Genetics at the Institute of Molecular BioSciences, Massey University, Palmerston North, New Zealand
... "COMET" assay was ...COMET assay was introduced by Singh et ...COMET assay, electrophoresis was performed under high alkaline (pH > 1 3) conditions for detecting DNA damage in single ...
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Amniotic Fluid Microviscosity Determined by Fluorescence Polarization: Methodology and Relation to Gestational Age
... Because there were important differ- ences in the period of gestation during which sequential samples were obtained, the data from individual pregnant women were grouped as follows: grou[r] ...
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