Formalin-fixed paraffin-embedded (FFPE)
Microsporidial keratitis retrospectively diagnosed by ultrastructural study of formalin-fixed paraffin-embedded corneal tissue: a case report
... in formalin- fixed paraffin-embedded (FFPE) corneal specimens that have already been diagnosed as another disease, it may be possible to retrospectively determine with accuracy the ...
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Gene-expression analysis of a colorectal cancer-specific discriminatory transcript set on formalin-fixed, paraffin-embedded (FFPE) tissue samples
... parallel, formalin-fixed, paraffin- embedded (FFPE) tissue samples are routinely collected for diagnostic purpose and are stored at room temperature in archives for several ...from ...
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Assessment of vascular endothelial growth factor in formalin fixed, paraffin embedded colon cancer specimens by means of a well-based reverse phase protein array
... Results: VEGF expression was studied by means of a well-based reverse phase protein array (RPPA) and immunohistochemistry in 69 colon cancer cases, and compared with various clinicopathologic factors. Protein lysates ...
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Sensitive and reliable detection of Kit point mutation Asp 816 to Val in pathological material
... Background: Human mastocytosis is a heterogenous disorder which is linked to a gain-of- function mutation in the kinase domain of the receptor tyrosine kinase Kit. This D816V mutation leads to constitutive activation and ...
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FIREVAT: finding reliable variants without artifacts in human cancer samples using etiologically relevant mutational signatures
... into formalin-fixed paraffin-embedded (FFPE) samples, 8-oxoG-mediated errors, chimeric reads, and others ...1130 FFPE samples had KRAS mutations that were validated as artifacts ...
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Identification of micro RNA expression profile related to recurrence in women with ESMO low risk endometrial cancer
... 7 formalin‑fixed paraffin‑embedded (FFPE) LREC primary tumors from women whose follow up showed recurrences (R + ) and in 14 FFPE LREC primary tumors from women whose follow up ...
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EGFR mutation status in Tunisian non-small-cell lung cancer patients evaluated by mutation-specific immunohistochemistry
... Methods: E746-A750 deletion and L858R mutations were screened in 50 unselected NSCLC formalin-fixed paraffin-embedded (FFPE) tissue samples. Mutation expression by IHC was evaluated by ...
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Prognostic significance of PD-L1 expression and CD8+ T cell infiltration in pulmonary neuroendocrine tumors
... AC: Atypical carcinoid; APCs: Antigen-presenting cells; CTLs: Cytotoxic lymphocytes; FFPE: Formalin-fixed paraffin-embedded; HNSCC: Squamous cell carcinoma of head and neck; IHC: Immunoh[r] ...
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PD-L1 in pancreatic ductal adenocarcinoma: a retrospective analysis of 373 Chinese patients using an in vitro diagnostic assay
... Representative, formalin-fixed, paraffin-embedded (FFPE) tissue blocks from the enrolled cases were retrieved from the tissue sample library of the pathology department of PUMCH along ...
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The rise of a novel classification system for endometrial carcinoma; integration of molecular subclasses
... on formalin-fixed, paraffin-embedded (FFPE) material and designate four molecular subclasses of ECs with distinct prognostic outcomes, analogous but not identical to the TCGA subgroups ...
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Integrating precision cancer medicine into healthcare—policy, practice, and research challenges
... Consortium; FDA: Food and Drug Administration; FFPE: Formalin-fixed paraffin-embedded; GA4GH: The Global Alliance for Genomics and Health; IBIS: International Breast Cancer Intervention [r] ...
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Prognostic Significance of Promoter Hypermethylation and Diminished Gene Expression of SYNPO2 in Melanoma.
... We proceeded with promoter methylation analysis of MCHR1 and SYNPO2 in an independent series of 113 formalin-fixed paraffin-embedded FFPE primary invasive cutaneous melanoma samples with[r] ...
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Immunohistochemistry of sarcolemmal membrane-associated proteins in formalin-fixed and paraffin-embedded skeletal muscle tissue: a promising tool for the diagnostic evaluation of common muscular dystrophies
... The successful IHC of sarcolemmal membrane-associated proteins in this study yields a promising tool for the diag- nostic evaluation of common MDs when combined with clinical findings and changes in muscle biopsy, ...
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Single-strand DNA library preparation improves sequencing of formalin-fixed and paraffin-embedded (FFPE) cancer DNA
... from FFPE material we obtain on average 900-fold more library molecules and improved sequence complexity from as little as 5 ng input ...DNA. FFPE DNA is highly fragmented, usually below 100bp, and up to ...
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Detection of HPV DNA in paraffin embedded cervical samples: a comparison of four genotyping methods
... Genotyping agreement for 60 paired cytology- and formalin-fixed and paraffin embedded FFPE specimens in the SUCCEED study; Onclarity, The BD Onclarity™ HPV Assay; Inno-LiPA, The Inno-LiP[r] ...
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High-risk HPV is not associated with epithelial ovarian cancer in a Caucasian population
... The study was a part of the Danish Pelvic Mass Study, a prospective, ongoing collection of blood and tumor tis- sue samples. Oral and written consent were given by each patient before enrollment, and the Danish Ethical ...
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Development and validation of an immunohistochemistry assay to assess glucocorticoid receptor expression for clinical trials of mifepristone in breast cancer
... Background: Glucocorticoid receptor (GR) activity has been associated with chemotherapy resistance and poor outcomes in patients with triple negative breast cancer (TNBC). The aim of this study was to develop an ...
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Residual tissue repositories as a resource for population-based cancer proteomic studies
... A 200 µL aliquot of 50:50 TFE:600 mM Tris was added to the de-paraffinized tissue, followed by 200 µL 50 mM ammonium bicarbonate (ABC). Two µL of phosphatase inhibitor cocktails 2 and 3 (Sigma-Aldrich) and Halt protease ...
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Detection of colorectal dysplasia using fluorescently labelled lectins
... buffer (PBS containing 1% foetal bovine serum, FBS), incubated for 15 min with AlexaFluor ™ − 647 (AF647) con- jugated WGA (Life Technologies, Paisley, UK) at 5 μg/ml, by clamping the two ends of the intestines at 20 °C. ...
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Immunohistochemical Expression of Nuclear Factor – Kappa B in Oral Squamous Cell Carcinoma, Oral Submucous Fibrosis and Normal Mucosa
... This study determined the feasibility of micro array gene expression analysis of formalin fixed tissue, and compared with gene expression of independent frozen tumor. This gene list which is highly ...
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