Formalin-Fixed Paraffin-Embedded Tissue
Analytical validation of a prognostic prostate cancer gene expression assay using formalin fixed paraffin embedded tissue
... Total RNA was isolated using the Roche High Pure RNA Paraffin kit (Roche, Basel, Switzerland). Samples with a minimum concentration of 12.5 ng/μl, quantified using a Nanodrop Spectrophotometer (ThermoFisher, Santa ...
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PITX3 promoter methylation is a prognostic biomarker for biochemical recurrence-free survival in prostate cancer patients after radical prostatectomy
... FFPET: Formalin Fixed Paraffin Embedded Tissue; HR: Hazard ratio; ISUP: International Society of Urological Pathology; LR: Likelihood ratio; mPITX2: Methylated PITX2; mPITX3: Methylated ...
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Sensitive and reliable detection of Kit point mutation Asp 816 to Val in pathological material
... In an initial experiment the most suitable primer combi- nation was determined according to Kwok et al. [7] by testing the three combinations on HMC-1 DNA and tonsil DNA as a wild-type control. Primer combination A ful- ...
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Recombinant "IMS TAG" proteins : a new method for validating bottom up matrix assisted laser desorption/ionisation ion mobility separation mass spectrometry imaging
... Mass spectrometry images of the distribution of proteins in fresh frozen and formalin fixed paraffin embedded tissue samples following in situ tryptic digestion were generated by isolati[r] ...
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Gene-expression analysis of a colorectal cancer-specific discriminatory transcript set on formalin-fixed, paraffin-embedded (FFPE) tissue samples
... Automated nucleic isolation protocols can hold possi- bilities in gene expression studies due to the fact, that several samples can be processed in parallel in a highly standardized manner with minimal hands-on time. ...
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Duplex PCR for Differential Identification of Mycobacterium bovis, M avium, and M avium subsp paratuberculosis in Formalin Fixed Paraffin Embedded Tissues from Cattle
... A presumptive diagnosis of bovine mycobacterial infec- tion in slaughtered animals is made on the basis of the his- topathology of lymph nodes and tissue specimens showing tuberculosis-like lesions and acid-fast ...
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CD68, CD163, and matrix metalloproteinase 9 (MMP 9) co localization in breast tumor microenvironment predicts survival differently in ER positive and negative cancers
... in formalin-fixed paraffin-embedded tissues of breast carcinomas represented in tissue microarray format using multiplexed quantitative immunofluorescence (QIF) in two independent Yale ...
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Validation of DNA methylation profiling in formalin-fixed paraffin-embedded colon and breast tissue using the Infinium HumanMethylation450 Microarray
... This study assessed the performance of matched fresh frozen (FF), FFPE DNA- unrestored and FFPE DNA-restored non-neoplastic colon tissue samples on the Infinium HumanMethylation450 microarray. The present study ...
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Concordance of metabolic enzyme genotypes assayed from paraffin-embedded, formalin-fixed breast tumors and normal lymphatic tissue
... all tissue samples were deparaf- finized by treatment with xylene; 1 mL of xylene was added to each microtube containing three to six slices of paraffin- embedded tissue and the microtubes ...
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Protease-resistant SOD1 aggregates in amyotrophic lateral sclerosis demonstrated by paraffin-embedded tissue (PET) blot
... Methods: Formalin-fixed and paraffin-embedded brain and spinal cord tissue from SOD1 G93A mice was first analyzed for the expression of SOD1, aggregated SOD1, ubiquitin, and p62 by ...
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Evaluation of formalin-fixed paraffin-embedded tissues in the proteomic analysis of parathyroid glands
... human tissue samples are often difficult to acquire or might not be large enough to accurately represent the tumor ...tissues. Formalin fixed paraffin embedded (FFPE) tissues, a common ...
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Validation of the MethylationEPIC BeadChip for fresh-frozen and formalin-fixed paraffin-embedded tumours
... Raw methylation data was normalised using Noob- normalisation with the R minfi package [15 – 17]. CpG sites with detection p value >0.01 were regarded as failed and were assigned as missing. Also, 43,254 CpG sites ...
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Identification by 16S rRNA Gene Analyses of a Potential Novel Mycobacterial Species as an Etiological Agent of Canine Leproid Granuloma Syndrome
... versus paraffin-embedded tissues was ...the formalin-fixed, paraffin- embedded tissues, since analyses of the two types of specimens were performed separately in two different ...
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Expression profiling with RNA from formalin-fixed, paraffin-embedded material
... with TaqMan assays from Applied Biosystems (data not shown). The optimum time of demodification was 20 min, longer times led to higher Ct values (not shown). The different protocols of RNA isolation from FFPE mate- rial ...
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Visualisation of HER2 homodimers in single cells from HER2 overexpressing primary formalin fixed paraffin embedded tumour tissue
... thick tissue sections were pre- pared from FFPE blocks of HER2 positive and nega- tive cancer samples (see Additional file 1 Figure ...the tissue sections were examined with LM, using fluor- escence ...
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Single-day HER2neu amplification assessment using chip-based digital PCR in formalin-fixed paraffin-embedded breast carcinoma tissue
... each tissue with macrodissection in the present study was about 70%–80%, which is a suitable percentage for the proper assessment of HER2 amplification from tumor tis- sue, without the hindrance of contamination ...
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High-risk HPV is not associated with epithelial ovarian cancer in a Caucasian population
... tumor tissue for ...study, formalin-fixed and paraffin-embedded (FFPE) EOC tumor samples from 198 consecutively in- cluded patients were ...
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Microsporidial keratitis retrospectively diagnosed by ultrastructural study of formalin-fixed paraffin-embedded corneal tissue: a case report
... A 68-year-old man was referred to Kindai University in 2004 with bilateral uveitis of unknown cause. The right eye had lost vision due to suspected Candida keratitis after penetrating keratoplasty, which was performed in ...
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miRNA expression profiling in formalin-fixed paraffin-embedded endometriosis and ovarian cancer samples
... Then the histopathological diagnosis was confirmed. The selection between endometriosis lesions and tumor area was done based on hematoxylin and eosin staining and macrodissected regions from serial unstained FFPE ...
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Molecular characterization of high risk human papillomavirus genotypes involved in invasive cervical cancer from formalin fixed, paraffin embedded tissues in Ouagadougou, Burkina faso
... of paraffin. To confirm the negative impact of paraffin, Steinau et ...and tissue samples already confirmed this disparity with preponderance of HR-HPV other than HPV 16 and 18 (Ouedraogo et ...
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