Free medium
A novel serum-free medium for the expansion of human mesenchymal stem cells
... serum-free medium [14], no published work had shown the ability to expand human MSCs under serum-free conditions for the long term in ...serum-free medium StemPro® MSC SFM was developed ...
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Constitutive activity of the metabotropic glutamate receptor 2 explored with a whole-cell label-free biosensor
... serum-free medium, cells did not at- tach to the bottom of the wells, did not proliferate and therefore did not show any remarkable level of impedance (data not ...serum-free medium was added ...
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HIV inhibits endothelial reverse cholesterol transport through impacting subcellular Caveolin 1 trafficking
... serum free medium and labeled with 1 μ Ci/ml [ 3 H] cholesterol and incubated for 36–48 ...serum free medium in the presence or absence of 50 μ g/ml HDL (Biomedical Technologies ...the ...
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Kinetic Characteristics of Glycine Transport by the Isolated Midgut of the Marine Shrimp, Penaeus Marginatus
... The percentage decrease in pHJglycine influx at each glycine concentration in Na+-free medium, relative to the transport rate in normal Na+ medium, therefore approximated the percent of [r] ...
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EFFECT OF CONCENTRATION OF PANTOTHENATE ON SELECTION OF A MUTANT FOR PANTOTHENATE SYNTHESIS IN SACCHAROMYCES CEREVISIAE
... T o study the nature of -the adaptation in pantothenate-free medium, dupli- cate tubes of culture 2154 were grown as before in synthetic medium with no added pantothenate a[r] ...
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Modulatory Effects of Human Bone Marrow-Derived Mesenchymal Stem Cells on Proliferation and Ultrastructural Changes in K562 Leukemic Cells
... complete medium enhanced the viability of the K562 cells in a dose- and time-dependent fashion (Figure ...serum-free medium (Figure ...serum- free medium (Figure ...
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Vaccinia Virus Requires Glutamine but Not Glucose for Efficient Replication
... replete medium, glu- tamine-free medium, or glutamine-free medium supple- mented with either 7 mM dimethyl-␣-ketoglutarate or 4 mM pyruvate, two TCA cycle intermediates that rescued ...
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Mechanisms of pH regulation in lamprey (Lampetra fluviatilis) red blood cells
... In addition to the experiments in HEPES-buffered, virtually CO2/HCO3~-free medium and in a medium containing 1 % CO2 and lOmmolF 1 HCO 3 ~, the effects of carbon dioxide and bicarbonate [r] ...
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The Coexistence of Cystic Fibrosis and Celiac Disease
... activity in the gluten-free medium which was not abolished by addition of gluten peptides to the medium. This behavior by intestinal mucosa has been described in patients in remission.â€[r] ...
9
Linkage of aerobic glycolysis to sodium potassium transport in rat skeletal muscle Implications for increased muscle lactate production in sepsis
... sodium-free medium. Preincubation in potas- sium-free medium followed by potassium re-addition also stimulated ouabain-inhibitable lactate ...incubation medium with pyruvate abol- ished ...
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Long term serial cultivation of mouse induced pluripotent stem cells in serum free and feeder free defined medium
... serum-free medium (Furue et al., 2005). This study reveals that in ESF5 medium an appropriate concentration of FGF-2 promotes differentiation of miPS cells from neural progenitor cells into ...
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Development of a surrogate potency assay to determine the angiogenic activity of Stempeucel®, a pooled, ex-vivo expanded, allogeneic human bone marrow mesenchymal stromal cell product
... The cell migration assay was performed using a Transwell two-chamber cell culture method and Transwell inserts (Corning, Cambridge, MA, USA) with an 8-μm-pore polycar- bonate membrane. HUVECs were serum starved for 18 h ...
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A simple and efficient method for the long-term preservation of plant cell suspension cultures
... Pi-free medium not permitting cell growth, and without the addition of growth inhibitors, did not generate long- lasting physiological and metabolic ...
12
Comparative in vitro study regarding the biocompatibility of titanium-base composites infiltrated with hydroxyapatite or silicatitanate
... standard medium with fetal calf serum (FCS), serum-free medium, specific osteogenic differen- tiation mediums: simple and complex (supplemented with growth ...
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MRI-guided targeting delivery of doxorubicin with reduction-responsive lipid-polymer hybrid nanoparticles
... culture medium, the KB cells with Gd-FLPNPs resulted in stronger DOX fluorescence than with Gd-LPNPs, in both the cytoplasm and ...folate-free medium, while it was ...the medium. When ...
12
Vesicle bound and free microRNAs in spent cell culture medium and biological fluids
... whether free extracellular miRNA ...culture medium of renal tubu- lar epithelial cells, as well as serum and urine from patients with systemic lupus erythematosus and healthy ...and free miRNA were ...
5
Synergistic effects on mesenchymal stem cell-based cartilage regeneration by chondrogenic preconditioning and mechanical stimulation
... This study aimed to determine the efficiency of a novel tissue engineering strategy, i.e., combined stepwise pre- conditioning and mechanical loading, in cartilage regen- eration or repair. Figure 1 shows the schematic ...
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Unsteady Free Convection from a Sphere in a Porous Medium with Variable Surface Temperature
... Abstract In this paper a transient free convection flow around a sphere with variable surface temperature and embedded in a porous medium has been considered. The temperature of the sphere is suddenly ...
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Numerical solution of Stokes problem for free convection effects in dissipative dusty medium
... time of particle phase (temperature of the particle phase relative to fluid is (1/e) times its initial value). In most studies of dusty fluids, some simplifying assumptions are usually made for dilute suspensions [8]. In ...
14
Waves excited at a free surface in a half infinite isotropic nonlinear elasic medium
... In this paper, the treatment is based on second order non- linear stress and further limited to the case of a direct-hit earthquake. The restriction of ‘direct-hit’ signifies small in- cidence angle of the incident waves ...
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