FRET and FLIM
HER2-HER3 dimer quantification by FLIM-FRET predicts breast cancer metastatic relapse independently of HER2 IHC status
... the FLIM-FRET assay (Figure 1A) were tested in FFPE SKBR3 cell pellets, mimicking the conditions of tumor tissue fixation and ...to FRET between the two proteins in close ...HER2-HER3 ...
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Epigenetic biomarker screening by FLIM-FRET for combination therapy in ER+ breast cancer
... Hormone-dependent gene expression involves dynamic and orchestrated regulation of epigenome leading to a cancerous state. Estrogen receptor (ER)-positive breast cancer rely on chromatin remodeling and association with ...
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Assessment of EGFR/HER2 dimerization by FRET-FLIM utilizing Alexa-conjugated secondary antibodies in relation to targeted therapies in cancers
... For FRET analysis, pixel-by-pixel lifetime determination was ...The FLIM images were obtained and the outputs files consist of all the fitting parameters recorded for each ...
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FLIM-FRET Imaging of Caspase-3 Activity in Live Cells Using Pair of Red Fluorescent Proteins
... the FLIM FRET of living cells via the confocal fluorescence ...estimate FRET efficiency and to analyze possible steric restrictions of the re- action between the substrate TagRFP-23-KFP and caspase-3 ...
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Intrinsic Cytoskeleton-Dependent Clustering of Influenza Virus M2 Protein with Hemagglutinin Assessed by FLIM-FRET
... With FLIM-FRET, we have recently shown clustering of HA with markers for outer and inner leaflet rafts (6, 22). Here, we have used the same approach to analyze whether M2 asso- ciates with rafts and whether ...
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A surface plasmon enhanced FLIM-FRET imaging approach based on Au nanoparticles
... the FRET effect will only be triggered in several nanometers’ range, it is a very sensitive distance measurement, or ‘the spectroscopic ...Therefore FLIM-FRET method can directly indicate if the gold ...
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Mapping Interactions between mRNA Export Factors in Living Cells
... performed FLIM-FRET ...average FRET efficiency of ...the FRET efficiency rose to ...the FLIM-FRET signal, images from HeLa cells co-expressing Chtop- ECFP and Nxf1-EYFP were ...
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Time-domain fluorescence lifetime imaging techniques suitable for solid-state imaging sensor arrays
... for FRET applications as well. Recently, a Bayesian FLIM analysis method was proposed, and it outperformed the commonly used MLE and LSM especially on noisy single-exponential data sets ...
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Signal peptide peptidase (SPP) dimer formation as assessed by fluorescence lifetime imaging microscopy (FLIM) in intact cells
... Using FLIM we were able to show that the donor fluorophore lifetime of the CFP tagged SPP construct in living cells significantly decreases when either a NH2- or COOH- terminally YFP tagged SPP construct is ...
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No better time to FRET: shedding light on host pathogen interactions
... Measuring FRET can be simplified considerably by determining the fluorescence lifetime rather than fluorescence intensities. Energy transfer from the donor to the acceptor accelerates the decay of donor ...
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Direct Interaction of Baculovirus Capsid Proteins VP39 and EXON0 with Kinesin-1 in Insect Cells Determined by Fluorescence Resonance Energy Transfer-Fluorescence Lifetime Imaging Microscopy
... Autographa californica multiple nucleopolyhedrovirus (AcMNPV) replicates in the nucleus of insect cells to produce nucleocap- sids, which are transported from the nucleus to the plasma membrane for budding through ...
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Novel Bioluminescent Activatable Reporter for Src Tyrosine Kinase Activity in Living Mice
... the FRET-Src biosensor has also been used as a preclinical tool to assess drug delivery and efficacy in tumors ...the FRET assay suffers from several weaknesses, includ- ing the need for an external ...
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An Indirect Immunoassay for Detecting Antigen Based on Fluorescence Resonance Energy Transfer
... in FRET because of several ...two FRET assem- bles in one system, in which luminescent CdTe QDs as FRET donors and GNPs serving as the acceptor with chymotrypsin as the linking bridge ...
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Muschielok, Adam (2010): Development and application of a quantitative analysis method for fluorescence resonance energy transfer localization experiments. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... lifetime, FRET efficiency and anisotropy, as well as the translational and rotational ...two FRET efficiency populations in the ...a FRET efficiency comparable to the values reported by Andrecka et ...
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NANOSCALE PHOTONIC DEVICES FABRICATED USING DNA NANOSTRUCTURES
... two FRET-based waveguides and a systematic approach to fabricate four different nanoparticle arrays have been ...demonstrated. FRET-based waveguides have been successfully designed, fabricated, and ...
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FRET analysis using sperm activating peptides tagged with fluorescent proteins reveals that ligand binding sites exist as clusters
... positive FRET signal upon binding to the receptor, which suggests that the speract receptor exists as an oligomer, at least as a dimer, or alternatively that a single speract receptor protein possesses multiple ...
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Accelerated super-resolution imaging with FRET-PAINT
... For FRET-PAINT, microtubules of the same area were imaged after injecting 30 nM donor (Donor_P1_A- lexa488) and 20 nM acceptor (Acceptor_P2_Cy5) ...of FRET-PAINT is much faster than that of ...and ...
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GPU acceleration of time-domain fluorescence lifetime imaging
... and time-correlated single-photon counting (TCSPC) methods. Due to superior temporal resolution and recent developments in advanced laser sources and cameras, TCSPC techniques have been the gold standard solutions for ...
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Conformational Heterogeneity in a Fully Complementary DNA Three Way Junction with a GC Rich Branchpoint
... the FRET distances listed in Table 1 as structural restraints. The FRET distances were imposed as the distance ± ...measured FRET distances, is the 2-free model. In other words, the FRET ...
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Fluorescence Resonance Energy Transfer (FRET) Based Sensors for Bioanalysis
... equilibrium. FRET between dextran-TR and FITC ConA-coated particles resulted in a fluorescence decrease of FITC by about 50% and a similar increase in the fluorescence of Texas ...The FRET sensing particles ...
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