fructose-2,6-bisphosphatase
Overexpression of 6 phosphofructo 2 kinase/fructose 2,6 bisphosphatase in mouse liver lowers blood glucose by suppressing hepatic glucose production
... Hepatic 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase is an important regulatory enzyme of glucose ...of fructose-2,6-bisphosphate, an allosteric activator of the glycolytic ...
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Nutritional regulation and tissue specificity of gene expression for proteins involved in hepatic glucose metabolism in rainbow trout (Oncorhynchus mykiss)
... enzymes 6-phosphofructo- 2-kinase/fructose-2,6-bisphosphatase (6PF-2K/F-2,6BPase), fructose-1,6-bisphosphatase (FBPase) and pyruvate kinase ...
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The Gluconeogenic Enzyme Fructose-1,6-Bisphosphatase Is Dispensable for Growth of the Yeast Yarrowia lipolytica in Gluconeogenic Substrates
... inhibition by AMP or fructose-2,6-bisphosphate. In some ar- chaea, Fbp activity was ascribed to an inositol monophos- phatase (62); since inositol monophosphatases are sensitive to Li ⫹ (46), we checked whether ...
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Case Report Fructose 1,6-bisphosphatase deficiency: clinical manifestations and genetic features in two Chinese patients
... FBPase deficiency is a very rare inborn error of gluconeogenesis, and was first identified in 1970 [1]. Till date, the number of reported cases in the world is still very limited and spo- radic cases have been reported ...
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Fructose-1,6-bisphosphatase loss modulates STAT3-dependent expression of PD-L1 and cancer immunity
... Our finding that FBP1 interacts with STAT3 suggests that FBP1 may regulate PD-L1 via STAT3. To test this hypothesis, we generated control and STAT3 knockout MIA PaCa-2 cells using CRISPR/Cas-9 (Figure 5A). We ...
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A summary of molecular genetic findings in fructose-1,6-bisphosphatase deficiency with a focus on a common long-range deletion and the role of MLPA analysis
... exon 2 (the first coding exon), we were able to generate a junction fragment with primers 5 ′ -taaaggtttccgc- gattcac-3 ′ (sn) and 5 ′ -gaccatcctggccaacac-3 ′ ...
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Reduction in sucrose contents by downregulation of fructose-1,6-bisphosphatase 2 causes tiller outgrowth cessation in rice mutants lacking glutamine synthetase1;2
... Rice mutants lacking cFBPase1 presented with severe reductions in growth, tiller number, and sugar (sucrose, glucose, and fructose) levels in the leaf blades (Lee et al. 2008; Koumoto et al. 2013). OscFBP1 was ...
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Metabolic regulation of calcium pumps in pancreatic cancer: role of phosphofructokinase-fructose-bisphosphatase-3 (PFKFB3)
... PFK1 activity can also be increased by fructose-2,6- bisphosphate (F26BP), a potent allosteric activator that drives glycolytic flux. F26BP is produced by phosphofructokinase-fructose-bisphosphatases ...
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Enhancement of photosynthetic capacity in Euglena gracilis by expression of cyanobacterial fructose-1,6-/sedoheptulose-1,7-bisphosphatase leads to increases in biomass and wax ester production
... (Nacalai Tesque, Kyoto, Japan). The resulting FAMEs were then purified with a silica cartridge column and finally eluted with 3 ml of elution solution (n-hexane:methyl acetate = 94:2 (v/v)) supplied by the ...
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The intracellular site of synthesis of fructose 1,6 bisphosphatase in rat liver : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Biochemistry at Massey University
... A more accurate es ti mate of FB Pase and al bumi n synthesi s was achi eved by pul s e l abel l i ng the l i ver v i a the portal vei n for 10 mi nutes . The subcel l u l ar fracti onati on scheme appl i ed to l i ver ...
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Original Article Fructose-1,6-bisphosphatase 1 can be a potential predictive marker for the prognosis of hepatocellular carcinoma patients
... Figure 6, we can find that patients whose total score more than 2 are more likely to die and total score more than 1 are apt to relapse than patients whose score less than 2 and ...
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Partial purification and characterisation of cytosolic Fructose-1, 6-bisphosphatase from Drymaria cordata
... From the data represented in fig.2, it becomes clear that the FBPase enzyme activity was appreciably increased at a narrow pH range from 6.5 to 8.5. The D. cordata FBPase exhibited optimum activity at a pH range ...
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Gluconeogenic Fructose 1, 6-bisphosphatase from Ginkgo biloba L.: Isolation and characterization
... the previous step was loaded onto a column (0.6 x 7.0 cm) of BioGel A-0.5m [before this experimentation, the column material was pre-equilibrated with 50 mM Tris-HCl buffer (pH 7.5) containing 0.2 mM ME.] Proteins were ...
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Hypoglycaemic Activity of Tissue cultured and Field grown Plants of Tinospora cordifolia
... A protocol was established for rapid clonal propagation of the valuable medicinal plant, Tinospora cordifolia. Best shoot induction was observed on MS basal medium supplemented with 4.36 µM KIN produces 2.32 0.1 cm ...
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Metformin reduces liver glucose production by inhibition of fructose-1-6-bisphosphatase.
... was administered to fasted (16 h) mice and after 60 min exposure, gastrocnemius (GAS) muscle biopsies were taken and assayed for (a-f) AMPK activation and downstream signaling by Western blotting and (g) levels of ...
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Succinic Acid Monoethyl Ester and Metformin Regulates Carbohydrate Metabolic Enzymes and Improves Glycemic Control in Streptozotocin-Nicotinamide Induced Type2 Diabetic Rats
... type 2 diabeteic model was ...doses 2, 4, and 8 µmol/g body weight (bw) respectively for 30 days, after which blood hemoglobin, glycosylated hemoglo- bin, plasma glucose and insulin, hexokinase, ...
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International practices in the dietary management of fructose 1-6 biphosphatase deficiency
... 1, 6 bisphosphatase (FBPase) deficiency (OMIM #229700), is a rare, autosomal recessive inherited disorder of gluconeogenesis ...of 6 months following severe metabolic acidosis probably with the same ...
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Fructose 1,6-bisphosphatase 2-Camk2 interaction is an integral mechanism of long-term potentiation
... purified as described in [21]), mouse anti-Fbp (1:500, Santa Cruz, sc-271799), rabbit anti-Camk2α (1:500, Sigma Aldrich, C6974), mouse anti-Camk2α (1:500, ThermoFisher Scientific, MA1-048), mouse anti- Camk2α pT286 ...
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Genome sequence of the Litoreibacter arenae type strain (DSM 19593T), a member of the Roseobacter clade isolated from sea sand
... with an optimum at 30 °C. Cells were successfully grown on marine agar (MA), nutrient agar (NA, weak growth), salt tolerance agar (STA, containing 1% (w/v) tryptone, 0.3% (w/v) yeast extract and 1.5% (w/v) agar ...
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Antihyperglycemic effect of short term arginyl fructose supplementation in subjects with prediabetes and newly diagnosed type 2 diabetes: randomized, double blinded, placebo controlled trial
... The diet information for the subjects was obtained using both a 24-hour recall method and a semiquantitative food frequency questionnaire that had been previously validated [14]. We used the former for analyses and the ...
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