fungal DNA
Detection of Fungal DNA in Human Body Fluids and Tissues during a Multistate Outbreak of Fungal Meningitis and Other Infections
... of fungal DNA for ...of fungal meningitis, and several protocols have been developed for molecular detection of ...fumigatus DNA in serum and whole blood ...of DNA from the pellet ...
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Occurrence of Fungal DNA Contamination in PCR Reagents: Approaches to Control and Decontamination
... identified fungal DNA contamination in critical, commercially available reagents required for nucleic acid amplifi- cation ...for fungal detection. The presence of traces of contaminating ...
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Quantification of Fungal DNA by Using Fluorescence Resonance Energy Transfer and the Light Cycler System
... of fungal DNA by using the TaqMan system (Perkin-Elmer, Foster City, ...of DNA amplified by PCR ...the fungal load in clinical samples was not determined by this ...
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Comparison of Six DNA Extraction Methods for Recovery of Fungal DNA as Assessed by Quantitative PCR
... QIAMP-S DNA extraction method on BAL fluid were unsuccessful because Aspergillus and other fungal DNA was detected in digest controls, indicating con- tamination of the ...this DNA extraction ...
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Development and Clinical Application of a Panfungal PCR Assay To Detect and Identify Fungal DNA in Tissue Specimens
... where fungal elements were visualized but no pathogen was ...parapsilosis DNA was detected from a nasopharyngeal biopsy specimen, but histologically the pathogen was identified as ...of DNA is likely ...
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Simple PCR Based DNA Microarray System To Identify Human Pathogenic Fungi in Skin
... of fungal DNA to which corresponding specific probes are not included in the ...detecting fungal DNA and the pattern of the latter identifying the fungus as ...representative fungal ...
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New Panfungal Real Time PCR Assay for Diagnosis of Invasive Fungal Infections
... invasive fungal infections (IFIs) is usually based on the isolation of the fungus in culture and histopathological ...the fungal species involved, increasing response ...After DNA amplification, a ...
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Comparison of Whole Blood, Serum, and Plasma for Early Detection of Candidemia by Multiplex Tandem PCR
... with DNA extracted from whole blood from 52/74 (70%) patients, accelerating the time to detection (blood culture flagging) and determination of the pathogenic species (by use of the API 32C system [bioMe ´rieux, ...
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Rapid Identification of Dimorphic and Yeast Like Fungal Pathogens Using Specific DNA Probes
... particular fungal organism (3, 14, 21, 26, 29, 30, 32, 33, ...universal fungal primers directed to the highly conserved ITS1 and ITS4 regions of ribosomal DNA allowed amplifica- tion of all ...
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Quantitation of Candida CFU in Initial Positive Blood Cultures
... their DNA can be detected by PCR. Also, DNA from dead organisms may be easier to access than that from living yeasts due to a leaky cell ...maximize fungal DNA burden per ...that ...
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A Novel Eukaryote Made Thermostable DNA Polymerase Which Is Free from Bacterial DNA Contamination
... or fungal pathogens, but we would like to propose the possi- bility of conducting the PCR-based detection of ...and fungal pathogens, a two-step usage of thermostable DNA poly- merases is ...
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DNA Microarray Based Detection and Identification of Fungal Pathogens in Clinical Samples from Neutropenic Patients
... the DNA microarray technology for the detection of fungal pathogens from cultured clinical ...the fungal rRNA genes of filamentous fungi but not of ...diluted fungal DNA samples for all ...
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Nutritional effects of indigenous arbuscular mycorrhizal associations on the sclerophyllous species Agathosma betulina
... AM fungal establishment. AM fungal ribosomal gene sequence analyses were used to identify some of the fungi within the roots, responsible for the nutritional ...AM fungal establishment (0 to 77 days ...
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From synthetic DNA to PCR product : detection of fungal infections using SERS
... the fungal DNA were selected for specific ...the DNA target and hence the probes used to functionalise the nanoparticles is of paramount importance for target specificity and to achieve multiplexed ...
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Detection and Identification of Fungal Pathogens by PCR and by ITS2 and 5 8S Ribosomal DNA Typing in Ocular Infections
... ribosomal DNA (rDNA) can be used to detect fungal pathogens in patients with ocular infections (endoph- thalmitis and ...detect fungal DNA. Fifty strains of 12 fungal species (yeasts ...
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Clinical Spectrum of Exophiala Infections and a Novel Exophiala Species, Exophiala hongkongensis
... Briefly, fungal cells on SDA were harvested with a sterilized cotton swab and suspended in 1 ml of autoclaved distilled ...water. DNA was then extracted from the fungal cells using the DNeasy plant ...
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Integration of Clustering and Multidimensional Scaling to Determine Phylogenetic Trees as Spherical Phylograms Visualized in 3 Dimensions
... high-throughput DNA sequencing techniques to identify microbial communities in the environment has led to a dramatic increase in the size of DNA sequence ...of fungal diversity have generally ...
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Early gut mycobiota and mother-offspring transfer
... ternal fungal DNA concentration/presence, the mode of delivery and maternal probiotics use were used as covar- ...the fungal DNA abundance and clinical ...
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PCR-DGGE analysis of fungal community in manufacturing process of a traditional Iranian cheese
... DNA/RNA extraction of fungal population. Two milliliters of milk samples were centrifuge for 10 min at 14,000 × g under refrigerated condition (4°C). The supernatant, that contains fat layer, was discarded ...
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One Fungus = One Name: DNA and fungal nomenclature twenty years after PCR
... important fungal groups have begun naming newly recognized genus-level clades with just one Ascomycota name, whether or not WKH IXQJXV H[KLELWV VH[XDO UHSURGXFWLRQ 7KH ¿UVW WKRURXJK H[SORUDWLRQ RI WKLV SUDFWLFH ...
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