furin cleavage site
A Furin Cleavage Site Inserted into the Spike Protein of SARS-CoV-2: A Structural Implication?
... potential cleavage site for furin protease, including four residues (Pro681, Arg682, Arg683 and Ala684) ...polybasic furin cleavage cite, ...polybasic furin cleavage ...
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A Single Point Mutation Creating a Furin Cleavage Site in the Spike Protein Renders Porcine Epidemic Diarrhea Coronavirus Trypsin Independent for Cell Entry and Fusion
... by furin was documented already in the early 1980s (9, 10), this cleavage occurs only in a subset of coronaviruses and does not liberate the putative fusion peptide at the N terminus of the ...
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Furin-Mediated Cleavage of the Feline Foamy Virus Env Leader Protein
... cellular furin-like protease, most likely furin itself, generating an Elp protein consisting of 127 amino acid ...The cleavage site fully conforms to the rules for an optimal furin ...
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The Furin Protease Cleavage Recognition Sequence of Sindbis Virus PE2 Can Mediate Virion Attachment to Cell Surface Heparan Sulfate
... PE2 cleavage resulted in a large reduction in binding efficiency compared with that of parental cleavage- competent ...PE2 cleavage was inhibited by replacing or deleting positively charged amino ...
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Synthesis, Assembly, and Processing of the Env ERVWE1/Syncytin Human Endogenous Retroviral Envelope
... Conclusion. The results presented here provide evidence that the syncytin/HERV-W envelope glycoprotein expressed in placental trophoblastic BeWo cells is synthesized as a gPr73 precursor which is specifically cleaved at ...
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Furin Cleavage of L2 during Papillomavirus Infection: Minimal Dependence on Cyclophilins
... exogenous furin in a variety of cell types (48, ...L2 cleavage and subse- quent infectivity are quite limited in HaCaT cell culture systems, FIG 1 Characterization of the PSTCD-L2 ...the furin ...
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Resuscitating Mutations in a Furin Cleavage-Deficient Mutant of the Flavivirus Tick-Borne Encephalitis Virus
... Generation of recombinant prM mutants. Inoculations of the mutant prM( ⌬ R88) intracerebrally into suckling mice and peripherally into an adult mouse both yielded point mutations in protein prM which either eliminated or ...
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Update of human and mouse matrix metalloproteinase families
... MMP13) were the first to be discovered and may be considered the prototypical MMPs. These enzymes can cleave interstitial collagens. MMP2 and MMP14 have the ability to cleave collagen as well, but are classified ...
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Engineering Glycoprotein B of Bovine Herpesvirus 1 To Function as Transporter for Secreted Proteins: a New Protein Expression Approach
... by furin, an endoproteinase localized in the trans-Golgi network. Cleavage by furin is a common mechanism for the activation of a number of viral fusion (F) ...that cleavage of the respective ...
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Mechanisms of Human Papillomavirus Type 16 Neutralization by L2 Cross-Neutralizing and L1 Type-Specific Antibodies
... a furin inhibitor, ...the furin inhibitor ...the furin cleavage site; the subsequent step of furin cleavage of L2 leads to exposure of the L2 17-36 polypeptide, which can ...
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Furin Is Involved in Baculovirus Envelope Fusion Protein Activation
... culture and in insects. The major envelope protein of HaSNPV BV, the product of Ha133, has also been identified and char- acterized (7; M. Westenberg and H. Z. Wang, personal com- munication). This protein turned out to ...
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The SARS-CoV-2 Spike Protein D614G Mutation shows Increasing Dominance and May Confer a Structural Advantage to the Furin Cleavage Domain.
... S1 furin cleavage ...the furin domain (RRARS, R682 to ...the furin domain whereas the G614 model predicted an alpha ...the cleavage domain of G614 model were found to better align with ...
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A Novel Activation Mechanism of Avian Influenza Virus H9N2 by Furin
... Chicken furin has higher processivity than human furin on Israel810 ...chicken furin has a higher processivity than human furin in cleaving H9N2 avian in- fluenza virus HA ...each ...
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Initiation of Duck Hepatitis B Virus Infection Requires Cleavage by a Furin-Like Protease
... proteolytic cleavage of the envelope protein by a furin-like proprotein ...of furin led to DCPD cleavage and increased virus ...to cleavage of the large envelope protein, and such viral ...
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Genetic Predisposition To Acquire a Polybasic Cleavage Site for Highly Pathogenic Avian Influenza Virus Hemagglutinin
... Accelerated nucleotide insertions in the presence of consecutive adenine residues. We then hypothesized that a stretch of adenine residues in the RNAseqHAclv region might affect the frequency of nucleotide insertions, as ...
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Proteolytic Cleavage of the Fusion Protein but Not Membrane Fusion Is Required for Measles Virus-Induced Immunosuppression In Vitro
... FIG. 5. The inhibitory activity of BJAB cells infected with a recombinant MV cleavage mutant (MV-Fcm) depends on exogenous trypsin treatment. (A) BJAB cells infected with MV (MOI, 0.1) ( ■ ) or MV-Fcm (MOI, 1) ( F ...
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Furin Cleavage Potentiates the Membrane Fusion-Controlling Intersubunit Disulfide Bond Isomerization Activity of Leukemia Virus Env
... whether furin cleavage of the Env precursor potentiates the isomerase to be ...a furin-inhibiting ...by furin cleavage in the ...
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Ty1 Proteolytic Cleavage Sites Are Required for Transposition: All Sites Are Not Created Equal
... Gag/PR cleavage site was determined previously (30; Lawler et ...this site by the amino acid residues AAGSAA centered about the Gag/PR cleavage site blocked ...two cleavage sites ...
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Polyprotein Processing Protease of African Swine Fever Virus: Purification and Biochemical Characterization
... the site located closer to the N terminus is cleaved first, releasing the final product p15 along with an intermediate precursor termed ...second site to produce p35 and an 8-kDa polypeptide that has not ...
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Molecular Characterization of Proteolytic Processing of the Gag Proteins of Human Spumavirus
... Analysis of peptide 4 illustrates the approach taken (Table 1). In the MALDI mass spectrum of the 11-mer peptide 4, a molecular ion at m/z 1,236.3 was observed in the control di- gestion with the enzymatically inactive ...
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