Fusion Peptide
Length Requirements for Membrane Fusion of Influenza Virus Hemagglutinin Peptide Linkers to Transmembrane or Fusion Peptide Domains
... these peptide linker regions is the requirement that they be capable of folding into a cell surface-expressed metastable conformation that can be subse- quently triggered by acidification to execute the obligate ...
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Mutations in the Fusion Peptide and Adjacent Heptad Repeat Inhibit Folding or Activity of the Newcastle Disease Virus Fusion Protein
... fusion peptide. Here we describe two mutants with changes in the fusion peptide sequence, G123A and ...not fusion, while G128L blocked surface ...a fusion-defective ...7. ...
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Studies of the membrane fusion activities of fusion peptide mutants of influenza virus hemagglutinin.
... of fusion, which may explain the apparent contradiction, since the latter are essential for studies of the mechanism of HA-mediated membrane ...the fusion peptide or the manner of membrane ...for ...
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An Antibody Directed against the Fusion Peptide of Junín Virus Envelope Glycoprotein GPC Inhibits pH-Induced Membrane Fusion
... membrane fusion by the class I envelope glycoproteins (reviewed in references 45 and 73) posits that the native complex exists in a metastable state that is established on proteolytic maturation of the ...
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Mutations in the fusion peptide and heptad repeat regions of the Newcastle disease virus fusion protein block fusion.
... 7654 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest Nonconservative mutations were introduced by site-specific mutagenesis into the fusion peptide and the adjacent hept[r] ...
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Mutagenesis and Nuclear Magnetic Resonance Analyses of the Fusion Peptide of Helicoverpa armigera Single Nucleocapsid Nucleopolyhedrovirus F Protein
... envelope fusion protein have been iden- tified in the budded virus (BV) of ...membrane fusion, and viral budding is mediated by the enve- lope glycoprotein GP64 (3, 17, 36, ...brane fusion protein, ...
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Mutational Evidence for an Internal Fusion Peptide in Flavivirus Envelope Protein E
... inducing fusion of the viral membrane with an intracellular membrane after uptake by ...cellular fusion proteins because of its distinct molecular architecture and apparent lack of involvement of coiled ...
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Atypical Fusion Peptide of Nelson Bay Virus Fusion-Associated Small Transmembrane Protein
... Membrane fusion plays important roles in biological pro- cesses such as vesicular trafficking, myoblast differentiation, and virus entry (1, 3, 18, 20, ...membrane fusion has been studied extensively with ...
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A Heptad Repeat in Herpes Simplex Virus 1 gH, Located Downstream of the α-Helix with Attributes of a Fusion Peptide, Is Critical for Virus Entry and Fusion
... by fusion with cell membranes; it requires gD as the receptor binding glycoprotein and the trigger of fusion, and the trio of the conserved glycoproteins gB, gH, and gL to execute ...internal fusion ...
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The Ectodomain of Herpes Simplex Virus Glycoprotein H Contains a Membrane α-Helix with Attributes of an Internal Fusion Peptide, Positionally Conserved in the Herpesviridae Family
... cell fusion and infectivity complementation activity, al- though in that instance there was a defect in cell surface ex- pression ...internal fusion peptide. Its replacement with the fusion ...
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Constancy and diversity in the flavivirus fusion peptide
... alence of the fusion peptide mutations. Sequence hetero- geneity is found with many other RNA viruses and is considered to result from both the lack of a proof-reading mechanism in virus-encoded RNA ...
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Mutagenesis of the fusion peptide-like domain of hepatitis C virus E1 glycoprotein: involvement in cell fusion and virus entry
... respectively. This plasmid was transfected into 293T cells to produce secreted sCD81/LEL. Next, cell lysates contain- ing WT or mutant proteins were incubated with equal amounts of concentrated sCD81/LEL followed by ...
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Conserved Glycine Residues in the Fusion Peptide of the Paramyxovirus Fusion Protein Regulate Activation of the Native State
... full fusion and is not blocked at ...cell-cell fusion mediated by the SV5 W3A and WR F proteins containing G3A and G7A ...Cell-cell fusion is observed as the transfer of red R18 and green CF from the ...
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Identification of the Fusion Peptide-Containing Region in Betacoronavirus Spike Glycoproteins
... Cell-cell fusion assays. Cell-cell fusion assays were performed as pre- viously described (37), with ...cell-cell fusion, 293T cells cotransfected with pFR-Luc, which contains a synthetic promoter ...
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Studies on the fusion peptide of a paramyxovirus fusion glycoprotein: roles of conserved residues in cell fusion.
... A SV40 F recombinant virus-infected cells expressing wt F, G3A, G7A, and G12A and cells infected with an irrelevant SV40 recombinant virus C expressing the influenza virus HA glycoprotei[r] ...
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Use of Single-Chain Antibody Derivatives for Targeted Drug Delivery
... a fusion peptide composed of a fully humanized anti-fAChR scFv antibody (called scFv35) and MAP and evaluated its specificity and cytotoxicity for fAChR-positive cell lines (FL-OH1 and ...MAP fusion ...
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Mechanisms of membrane fusion
... hydrated peptide-lipid membranes of the present study may reflect the intrinsic tendency of these peptide to "break" a-helix and form inter-molecular ...its fusion peptide (Daniels et ...
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Soluble Respiratory Syncytial Virus Fusion Protein in the Fully Cleaved, Pretriggered State Is Triggered by Exposure to Low-Molarity Buffer
... paramyxovirus fusion (F) glycoprotein is anchored in the virion membrane in a metastable, pretriggered ...hydrophobic fusion peptide into the target cell membrane, then folds back on itself to bring ...
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Functional Study of the C-Terminal Part of the Hepatitis C Virus E1 Ectodomain
... the fusion step between the viral and cell ...the fusion peptide of the viral fusion protein lead to a loss of activity and a consequent loss of virus entry, they can also affect additional ...
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An HIV-1 Mini Vaccine Induced Long-lived Cellular and Humoral Immune Responses
... results, fusion of P24 and Nef for candidate vaccine design was considered ...a fusion peptide candidate vaccine based on the most conserved and immunogenic sequences in proteins from HIV P24 and Nef ...
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