G2/M checkpoint
Role of garlic derived Allyl sulfide in inducing g2/m checkpoint arrest and apoptosis
... the G2/M phase checkpoint arrest and direct or indirect induction of ...at G2/M checkpoint or inducing apoptosis in different types of cancer ...
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Overexpression of the Drosophila ATR homologous checkpoint kinase Mei-41 induces a G2/M checkpoint in Drosophila imaginal tissue
... Overexpression of lok, however, had a similar effect as mei-41 overexpression (Fig. 4a, b), which, at first glance, came as a surprise: cell cycle re-entry appeared delayed even at 6 h after irradiation, i.e. the number ...
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Early G2/M checkpoint failure as a molecular mechanism underlying etoposide induced chromosomal aberrations
... early G2/M checkpoint after treatment with low concentrations of etoposide has been lost, executed mitosis with etoposide-induced DNA double-strand breaks, and 2-color FISH signals located on either ...
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Suppressors of Cdc25p Overexpression Identify Two Pathways That Influence the G2/M Checkpoint in Fission Yeast
... the checkpoint response to unreplicated DNA: Our results suggest that Chk1p and 14-3-3 proteins could function in the checkpoint response to unreplicated DNA by inhibiting Cdc25p activity, which prevents ...
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Inhibition of Methyltransferases Results in Induction of G2/M Checkpoint and Programmed Cell Death in Human T-Lymphotropic Virus Type 1-Transformed Cells
... AdOx-induced loss of cell viability in HTLV-1-transformed cell lines. During the course of the experiments described above, we observed that AdOx dramatically inhibited the growth of HTLV-1-transformed cells in culture. ...
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RAC1 GTPase plays an important role in γ irradiation induced G2/M checkpoint activation
... Figure 4 Rac1 inhibition abolishes IR-induced activation of both ATM and ATR signaling. (A) MCF-7 cells were treated with/ without 20-Gy IR in the presence or absence of 100 μ M NSC23766 and incubated for 1 hour ...
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<p>UBE2T promotes proliferation via G2/M checkpoint in hepatocellular carcinoma</p>
... in G2/M phase and a decrease in the percentage of cells in G1 phase, which indicated a cell cycle arrest at the G2/M ...in G2/ M phase decreased following UBE2T ...the ...
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NSCLC cells demonstrate differential mode of cell death in response to the combined treatment of radiation and a DNA- PKcs inhibitor
... robust G2/M checkpoint, (iii) increased aberrant mitosis followed by mitotic catastrophe specifically in H1299, (iv) dramatically induced autophagy in A549 and (v) IR-induced senescence specifically ...
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Down regulation of Chk1 by p53 plays a role in synergistic induction of apoptosis by chemotherapeutics and inhibitors for Jak2 or BCR/ABL in hematopoietic cells
... Chk1-mediated G2/M checkpoint activation through mechanisms involving activation of GSK3 [16, ...the G2/M phase and drastically enhanced apoptosis, as shown in Figure ...the ...
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Response gene to complement 32 regulates the G2/M phase checkpoint during renal tubular epithelial cell repair
... critical G2/M checkpoint regulator, RGC-32 participates in cell cycle ...in G2/M arrest through its formation of a protein complex with polo-like kinase 1 and phosphorylation in vitro ...
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Cell cycle checkpoint in cancer: a therapeutically targetable double-edged sword
... Besides its key role in the G2/M checkpoint, Wee1 kinase controls proper timing of mitosis onset by perform- ing inhibitory phosphorylation of Cdk1 [26]. Accordingly, using sarcoma cell lines and ...
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Original Article SERPINA3 promotes endometrial cancer cells growth by regulating G2/M cell cycle checkpoint and apoptosis
... regulating G2/M checkpoint in cell cycle and inhibited cells apoptosis, and we further uncovered that the pro-proliferative effect of SERPINA3 on EC was likely ascribed to the activation of ...
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<p>Sp1 contributes to radioresistance of cervical cancer through targeting G2/M cell cycle checkpoint CDK1</p>
... of G2/M during the course of DNA damage, and the cells are more susceptible to the toxic effects of radiation in the G2/M ...the G2/M phase. 28 Therefore, targeting the ...
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Murine Wee1 Plays a Critical Role in Cell Cycle Regulation and Pre-Implantation Stages of Embryonic Development
... cycle checkpoint induced by γ-irradiation and died of apoptosis before embryonic (E) day ...the G2/M checkpoint compared with other lower ...
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Zinc oxide nanoparticles-induced epigenetic change and G2/M arrest are associated with apoptosis in human epidermal keratinocytes
... the G2/M checkpoint, which was followed by reduced HaCaT cell ...at G2/M that was associated with epigenetic changes and accompanied by p53-Bax mitochon- drial pathway-mediated ...
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Targeting WEE1 to enhance conventional therapies for acute lymphoblastic leukemia
... immunoblotting analysis was performed on BV-173 cell line. The combination treatment of AZD-1775 (185 nM) and bosutinib (50 nM) enhanced γH2AX, the marker of DNA damage. The combination also perturbed the G2/ ...
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UV Irradiation Causes the Loss of Viable Mitotic Recombinants in Schizosaccharomyces pombe Cells Lacking the G2/M DNA Damage Checkpoint
... Figure 6.—Significance of the recombination phenotypes of UV-irradiated cells. Most spontaneous and UV-irradiated Ade ⫹ recombinants arise after plating. The recombinants on irradiated plates include both spontaneous ...
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Non-Classical P38 Map Kinase Functions: Cell Cycle Checkpoints and Survival
... a G2/M cell cycle checkpoint, the survival effect of p38 MAPK in re- sponse to DNA damage could be explained by its effect in facilitating DNA repair prior to the continua- tion through mitotsis, ...
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Checkpoint kinase inhibitor AZD7762 strongly sensitises urothelial carcinoma cells to gemcitabine
... For immunofluorescence, cells cultured on coverslips were treated as indicated for 24 or 48 h. Cells were fixed with 4% formaldehyde and permeabilized with 0.3% Triton X- 100 in PBS for 10 min at room temperature (RT). ...
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Transcriptome analysis identified a novel 3-LncRNA regulatory network of transthyretin attenuating glucose induced hRECs dysfunction in diabetic retinopathy
... μ M TTR treatment or endogenous TTR overexpression both repressed blood vessel tube forming capability of hyperglycemic cultured human retinal endothelial cells (hRECs), by attenuating pro-angiogenic genes ...
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