Galectin-1 gene knockdown by small interference RNA
RNA Interference-Mediated Knockdown on DKC1 Gene in Chemosensitized Colorectal Cancer Cell Lines
... To ensure RNAi efficacy at the protein level, Western blot was performed 72 hours post-transfection. Cell lysates were harvested using RIPA buffer containing 25 mM Tris-HCl (pH 7.6), 150 mM NaCl, 1% NP-40, ...
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Identification of chemosensory receptor genes in Manduca sexta and knockdown by RNA interference
... systemic RNA interference can be induced in ...each gene tar- geted, together, these results will prove useful for future functional studies of ...
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An Insight To RNA Interference Based Gene Silencing
... Table 1. It may be possible to exploit RNA interference in ...virus knockdown of host receptors and coreceptors for HIV,the silencing of hepatitis A and hepatitis B genes, silencing of ...
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A CRISPR Interference System for Efficient and Rapid Gene Knockdown in Caulobacter crescentus
... pasteurianus CRISPRi system inhibited growth when targeting gcrA or ctrA only when the sgRNA was complementary to the nontemplate strand between the transcriptional start site and the start codon (Fig. 1B to D; Fig. ...
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Gene-Specific Inhibition of Reovirus Replication by RNA Interference
... expressing small interfering RNAs (siRNAs) specific for transcripts encoding the 2, NS, and NS proteins of strain type 3 Dearing ...type 1 Lang, were significantly decreased in 293T cells stably ...
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CAPN5 gene silencing by short hairpin RNA interference
... The gene silencing effect of four different shRNA plasmids that target CAPN5 was ...tested. RNA and protein expression was determined using quantitative RT-PCR and immunoblot ...CAPN5 RNA in a stable ...
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RNA interference-mediated knockdown of voltage-gated sodium channel (MpNav) gene causes mortality in peach-potato aphid, Myzus persicae
... VGSC gene expression, through application of complementary dsRNA, caused significantly high larval mortalities and severe developmental arrest in the red flour beetle Tribolium castaneum, a coleopteran insect pest ...
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Chromosome-Wide Analysis of Gene Function by RNA Interference in the African Trypanosome
... 4A). Knockdown of GRASP55 and dolichoylphosphorylman- nose (DPM) synthase, part of the Golgi matrix system and responsible for generation of dolichol-P-mannose, a mannosyl ...RNAi knockdown in two cells ...
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Knockdown of the Chromatin Remodeling Gene \u3ci\u3eBrahma\u3c/i\u3e by RNA Interference Reduces Reproductive Fitness and Lifespan in Common Bed Bug (Hemiptera: Cimicidae)
... found gene knockdown from shRNAs (short hairpin RNAs) in multiple generations in ...vitellogenin gene also caused the abdominal inflation in bed bugs ( Moriyama et ...
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Oncolytic adenovirus mediated Survivin knockdown by RNA interference suppresses human colorectal carcinoma growth in vitro and in vivo
... Survivin RNA interferences ...cancer gene therapy ...exogenous gene, which offered a possibility for treatment of carcinomas with both onco- lytic adenovirus and specific gene targeted ...
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Ebolavirus Proteins Suppress the Effects of Small Interfering RNA by Direct Interaction with the Mammalian RNA Interference Pathway
... Since assays for the measurement of viral protein are not routinely performed in biosafety level 4 (BSL4) containment, where this experiment was conducted, we instead evaluated viral load by plaque assay to assess the ...
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Enhanced Gene Silencing in Cells Cured of Persistent Virus Infection by RNA Interference
... Ge ´nomique virale et vaccination, 25 rue du Dr Roux, 75015 Paris, France 3 Received 30 September 2009/Accepted 5 April 2010 We compared HEp-2-derived cells cured of persistent poliovirus infection by RNA ...
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RNA interference is not involved in natural antisense mediated regulation of gene expression in mammals
... different RNA-modifica- tion ...of RNA duplexes in the cytoplasm of cells, and to detect Dicer products involved in processing of ...cytoplasmic RNA duplexes or engage- ment of the RNAi ...
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Selection of highly efficient small interference RNA (SiRNA) targeting mammalian genes
... include gene function analysis and target ...efficient small interference RNA (siRNA) sequence with maximum target specificity for mammalian RNAi is one of important topics in recent ...
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Phosphonium polymethacrylates for siRNA delivery: effect of polymer and RNA structural parameters on polyplex assembly and gene knockdown
... mRNA knockdown was observed in HeLa cells when siRNA was replaced with multimerised liRNA demonstrating a strong effect of RNA structure on the efficiency of RNA ...and RNA - RNA ...
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Rna Interference As A Gene Silencing Tool To Control Tuta Absoluta In Tomato (solanum Lycopersicum)
... of RNA interference (RNAi), in which small RNAs can rapidly cause post-transcriptional specific gene silencing, has become a powerful tool for analysing gene function in a variety of ...
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RNA interference mediated knockdown of 3, 4 dihydroxyphenylacetaldehyde synthase affects larval development and adult survival in the mosquito Aedes aegypti
... In the present study, it was found that the expression of DOPAL synthase was the highest in white female pupae and mainly distributed in the cuticle (Fig. 5). The results suggest that DOPAL synthase may be involved in ...
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Post transcriptional inhibition of hepatitis C virus replication through small interference RNA
... Previous reports have demonstrated that NS3 and NS5B is essential for viral replication and therefore, pro- vides an attractive target for development of antiviral agents [29-31]. In this study, we showed that siRNAs can ...
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RNA Interference against Animal Viruses: How Morbilliviruses Generate Extended Diversity To Escape Small Interfering RNA Control
... Statistical analyses. The results of the tests with the firefly luciferase reporter system were compared with those of the nonparametric Wil- coxon test (55) and were adjusted by use of the Holm test (27). To assess the ...
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Small-interference RNA-mediated knock-down of aldehyde oxidase 1 in 3T3-L1 cells impairs adipogenesis and adiponectin release
... Total RNA prepared from subcutaneous and visceral adi- pose tissue of five donors was used for real-time ...reference gene b-actin (or porphobilinogen deaminase (PBGD)) was ...
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