Gel-filtration chromatography
Purification of protease from Coriandrum sativum using ion-exchange chromatography and gel filtration method
... using gel filtration chromatography Further purification process was carried out using gel filtration involving Agilent ZORBAX column (GF250) (Agilent ® technologies, ...
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“Partial Purification and Characterization of a Galactose Specific Lectin from Chrysophyllum cainito L, a Plant that Shows Hypoglycemic Activity” by Deepti Madayi, Sreekala S, Manju Mohan, Deepthi V.C, Anusha T.S, Elyas K K, India.
... Figure 1: Hemagglutination activity of different fractions obtained through ammonium sulphate precipitation. Blood grouping sera was used as positive control and PBS without lectin extract was used as the negative ...
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Proteins specified by herpes simplex virus. XIII. Glycosylation of viral polypeptides.
... Sephadex G50 gel filtration chromatography of glycopeptides obtained by Pronase digestion of: a a total lysate of uninfected cells labeled with D- ["Clglucosamine from 4 to 12 h after mo[r] ...
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Pulmonary and urinary clearance of atrial natriuretic factor in acute congestive heart failure in dogs
... Abbreviations used in this paper: ANF, atrial natiuretic factor; CHF, congestive heart failure; CO, cardiac output; FE, fractional excretion; GFC, gel filtration chromatography; GFR, glo[r] ...
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Secretion of insulinlike growth factor I and insulinlike growth factor binding proteins by murine bone marrow stromal cells
... cycloheximide. Gel filtration chromatography under denaturing conditions of TC-1 conditioned medium demonstrated two major peaks of apparent IGF-I immunoreactivity with molecular weights of ...
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ISOLATION AND CHARACTERIZATION OF SECONDARY METABOLITES FROM MARINE ORGANISMS AND DEVELOPMENT SYNTHETIC METHODOLOGIES
... As part of our ongoing investigation on bioactive compounds from marine organisms we describe the isolation of bastadin alkaloids from the sponge Dendrilla cactos. The freshly collected sponge was soaked in methanol at ...
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Study of Extraction, Purification and Characterization of Urease Enzyme from some plant Leguminales
... using gel filtration chromatography The next step was to purify the urease from the Lagonychium farctum seeds after the ion exchange step, loaded 10 mL of partially purified enzyme to the Sephadex ...
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Leucine Aminopeptidase from Arachis hypogaea L. Seeds Partial Purification and Characterization
... Gel filtration chromatography using sephadex G-100 column was used for purification of partially purified enzyme. The height of 120 cm in a glass column with an internal diameter of 2.0 cm were used ...
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Determination of viral proteins present in the human immunodeficiency virus type 1 preintegration complex.
... Viral DNA can be purified from these extracts by gel filtration chromatography and sucrose gradient sedimentation as a part of a nucleoprotein complex containing integrase as the only vi[r] ...
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Symbiotic “Archaezoa” of the Primitive Termite Mastotermes darwiniensis Still Play a Role in Cellulase Production
... individually from the midguts (termite origin) and hindguts (protozoa origin) by gel filtration chromatography (25). Re- cently, Li et al. (9) attempted to purify the protozoa-derived cellulase of M. ...
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Unraveling essential cellulosomal components of the (Pseudo)Bacteroides cellulosolvens reveals an extensive reservoir of novel catalytic enzymes
... by gel filtration chromatography using a preparative chromatography system for laboratory-scale protein purification (Äkta start; GE Healthcare, Upp- sala, ...Increase gel ...
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Purification and characterization of L-asparginase producing bacteria MNTG-7
... Several techniques have been described for recovery and purification of L-asparaginase from different sources. The present study was undertaken to recover and purify L-asparaginase from mutated MNTG-7 cell line using ...
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Chromatographic Profiles of Umami Fractions from Indonesian Commercial Salty Soy Sauce
... The four fractions also have relatively high absorbances at 214 nm which indicated the presence of compounds having peptide bonds and carboxylic groups, i.e., amino acids and peptides, in all four fractions. The pattern ...
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Effectiveness of Selected Reaction Monitoring for rapid assay of Cypermethrin Residue in Perilla Leaves
... The SRM process employed in this work led to the isola- tion of cypermethrin peaks from the matrix without the GPC or column chromatography pretreatments. The peak area ratios of the cypermethrin mass chromatogram ...
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Metalloproteases of human articular cartilage that digest cartilage proteoglycan at neutral and acid pH
... Sedimentation, gel chromatography, sodium dodecyl sulfate-gel electrophoresis, and immunodiffusion studies of digests of isolated proteoglycan fraction produced by the partially purified[r] ...
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The prevalence of hypertension and abnormal kidney function in children with sickle cell disease –a cross sectional review
... Renal consequences of SCD are evident in early life, and can involve multiple segments of the nephron. Im- paired urine concentrating ability is almost universally present early in childhood. Hematuria and overt papil- ...
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Purification and characterization of the DNA polymerase and RNase H activities in Moloney murine sarcoma-leukemia virus.
... Moloney MSV DNA polymerase RNase H-I, purified by Sephadex G-100 gel filtration followed by phosphocellulose, polyA oligodT-cellulose, and DEAE-cellulose chromatography, transcribed hete[r] ...
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Biochemical isolation and purification of ovulation-inducing factor (OIF) in seminal plasma of llamas
... The purified protein elicited a preovulatory LH surge followed by ovulation and corpus luteum formation in llamas after intramuscular administration. The chroma- tographic strategy used in this study revealed a single ...
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Cold Urticaria: RECOGNITION AND CHARACTERIZATION OF A NEUTROPHIL CHEMOTACTIC FACTOR WHICH APPEARS IN SERUM DURING EXPERIMENTAL COLD CHALLENGE
... challenged arm of each patient appeared in a high molecular-weight region, as assessed by the difference in activity recovered after Sephadex G-200 gel filtration of the paired lesional and control ...
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Carapanosins D—F from the seeds of andiroba ( Carapa guianensis , Meliaceae) and their effects on LPS-activated NO production
... Column chromatography was carried out over silica gel (70-230 mesh, Merck) and MPLC was carried out with silica gel (230-400 mesh, ...column chromatography were monitored by TLC (silica ...
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