Gene fusion
JAZF1/SUZ12 gene fusion in endometrial stromal sarcomas
... JAZF1/SUZ12 fusion tran- scripts ...JAZF1/SUZ12 gene fusion, these cases were negative in their FFPE ...JAZF1/SUZ12 gene fusion ...JAZF1/SUZ12 fusion se- quence, which was ...
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Identification of gene fusion transcripts by transcriptome sequencing in BRCA1-mutated breast cancers and cell lines
... a gene fusion junction would be align- ing at the exon ...a gene fusion candidate read ...summarized. Fusion partners with greater than or equal to three unique GFCRs were considered a ...
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The birth of a human specific neural gene by incomplete duplication and gene fusion
... For five human samples that showed copy number vari- ation, we performed fluorescent in situ hybridization (FISH) on chromosomal metaphase spreads for validation and cyto- genetic characterization (Fig. 2b). In all ...
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TMPRSS2:ERG gene aberrations may provide insight into pT stage in prostate cancer
... TMPRSS2:ERG gene fusion. This fusion is important not only for its high prevalence (or combining with other biomarkers), sensitiv- ity and specificity in early diagnosis of PCa [7, 8], but also in ...
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Heterogeneity in the inter tumor transcriptome of high risk prostate cancer
... We identified a tandem duplication genotype in the ETS-negative tumor T4 that appears to be recurrent at a low level in prostate, breast, and ovarian cancers. In T4, tandem duplications resulted in several potentially ...
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Xp11.2 translocation renal cell carcinoma occurring during pregnancy with a novel translocation involving chromosome 19: a case report with review of the literature
... BCL3 gene is not the novel gene fusion partner of TFE3 in the case herein ...the gene fusion partner of TFE3 at the ...different gene fusions may overlap ...translocation ...
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Driver Oncogenes but Not as We Know Them: Targetable Fusion Genes in Breast Cancer.
... real fusion events from false positives are considerable and have limited large-scale discovery of intergenic ...making fusion identification in breast cancers a more profitable exercise ...ETV6-NTRK3 ...
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Analysis of Promoter Function in Aspergillus fumigatus
... other fusion gene ...Additionally, fusion genes FIG 1 Map of promoter-reporter fusion gene ...promoter-reporter gene construct consisted of the ...reporter gene, followed ...
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Clinical and epidemiological study of EGFR mutations and EML4-ALK fusion genes among Indian patients with adenocarcinoma of the lung
... EML4-ALK gene fusions are present in lung adeno- carcinomas from Indian patients, and the 3% incidence of EML4-ALK gene fusion in EGFR mutation-negative cases is similar to what has been observed in ...
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Case Report Gene alteration of rosette-forming glioneuronal tumor in a supurasellar lesion
... important gene mediated activation of the RAS, MAPK1/ERK2, MAPK3/ERK1 and MAPK path- ...(gene fusion and missense mutation) were noted in 82/96, FGFR1 mutation in 4/96, and KRAS mutation in 2/96 ...
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Metabolic engineering of Agrobacterium sp. strain ATCC 31749 for production of an α-Gal epitope
... contains a HindIII site. The amplified pGEM-T easy vector with homologous regions of crdS was self-ligated to form pT-crdSh. pT-crdSh was digested using SacI and KpnI, and the resulting fragment containing crdS homology ...
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Genetic Analysis of the Major Capsid Protein of the Archaeal Fusellovirus SSV1: Mutational Flexibility and Conformational Change
... protein gene vp3, the vp1 gene does not tolerate deletion or transposon ...vp1 gene and its proteolysis for virus function, we developed techniques for site-directed mutagenesis of the SSV1 genome ...
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Advances in Fusion Gene Research and Fusion Gene Families in Hematological Malignancies
... Fusion genes (FGs) are major molecular biological abnormalities in hematological malignancies and have become well-established molecular markers for disease classification, risk stratification, and targeted ...
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Transactivator IE1 Is Required for Baculovirus Early Replication Events That Trigger Apoptosis in Permissive and Nonpermissive Cells
... fected with dsRNA and infected 24 h later with wt/lacZ, an AcMNPV recombinant in which a polh promoter-driven lacZ reporter replaced the polh gene (Fig. 1). Immunoblot analysis indicated that control egfp dsRNA ...
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Unraveling the diversity of sedimentary sulfate-reducing prokaryotes (SRP) across Tibetan saline lakes using epicPCR
... rRNA gene PCR amplicons and epicPCR products of fused 16S rRNA plus dsrB gene, respectively, with Proteobacteria , Firmicutes, and Bacteroidetes being the dominant phyla in both ...
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Molecular characterization of partial fusion gene and C terminus extension length of haemagglutinin neuraminidase gene of recently isolated Newcastle disease virus isolates in Malaysia
... the fusion protein cleavage site between residues 113 and 116 at the C- terminus of the F2, as well as phenylalanine at residue 117 of F1 and carboxyl terminus amino acid extension length which varies due to the ...
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Dual-Tagging Gene Trap of Novel Genes in Drosophila melanogaster
... Figure 4.—Correlation between the degree of eye pigmentation and the level of Gal4 expression in Dro- sophila strains with a gene- trap-vector insertion. (A) Relationships between the degree of eye pigmenta- tion ...
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JAFFA: High sensitivity transcriptome-focused fusion gene detection
... The genomic coordinates of each breakpoint were found and the genomic gap size calculated. In some cases, the gap was very small (less than 10 kb) indicating that the candidate was likely to be a false positive, ...
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Functional consequences of a gene duplication and fusion event in an arginine kinase
... D2–NusA–His-tag fusion protein produced some soluble activity, although a much higher yield was obtained by unfolding and refolding protein from inclusion ...amino-terminal fusion construct) and, therefore, ...
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Orthopoxvirus fusion inhibitor glycoprotein SPI-3 (open reading frame K2L) contains motifs characteristic of serine proteinase inhibitors that are not required for control of cell fusion.
... SPI-3 gene as described previously (39, 40) by recombinant ...range gene, was made in a similar fashion between primers RM139 and RM190 ...extensive fusion of the cell monolayer due to the ...
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