Gene knockout
<p>The journey from gene knockout to clinical medicine: telotristat and sotagliflozin</p>
... Abstract: Gene knockout has been a powerful technique to evaluate the physiologic role of selected gene ...high-throughput gene knockout technology and went further in designing agents ...
8
Heritable Gene Knockout in Caenorhabditis elegans by Direct Injection of Cas9–sgRNA Ribonucleoproteins
... Cho, S. W., J. Lee, D. Carroll, J. S. Kim, and J. Lee, 2013b Heritable gene knockout in Caenorhabditis elegans by direct injection of Cas9-sgRNA ribonucleoproteins. Genetics 195: 1177 – 1180. Cong, L., F. ...
5
Multiplexed CRISPR/Cas9 gene knockout with simple crRNA:tracrRNA co-transfection
... mediated gene knockout is a powerful tool for genome editing with the ability to target multiple genes ...multiplexed gene knockout system using CRISPR/Cas9 that is both simple and robust in ...
11
The Changing Regulation of Autophagy in Atherosclerosis in ApoE Gene Knockout Mice
... Abstract: Aim To investage the changes of autophagy in different stages of atherosclerosis (AS) in Apolipoprotein E gene knockout (ApoE -/- ) mice. Methods 6-weeks-old male ApoE -/- mice (No.=40) were ...
5
Outcome of urogenital infection with Chlamydia muridarum in CD 14 gene knockout mice
... initiate or influence inflammatory responses. Other possi- ble chlamydial PAMP that could engage CD14 exist. For example, it has been shown that CD14 binds heat shock proteins (hsp) [14] and the chlamydial hsp has been ...
6
A Targeted Gene Knockout in Drosophila
... yellow gene of Drosophila melanogaster. Because only a single gene was targeted, further work was required to show whether the method could be extended to become generally useful for gene ...
6
Transcriptome Analysis of Brain Tissues in a MeCP2-Null Rat Model of Rett Syndrome
... Genes significantly affected by MeCP2 gene knockout in the rat hippocampus. (A) 341[r] ...
16
Artificial bee colony and dynamic flux balance analysis for microbial production
... On the other hand, under anaerobic condition, lactate, acetate, ethanol, succinate and formate in E. coli are the yields of glucose mixed acid fermentation. Thus, E. coli is highly recommended since it is high ...
36
Engineering Corynebacterium glutamicum triggers glutamic acid accumulation in biotin-rich corn stover hydrolysate
... bioY gene knockout and glutamic acid synthesis enhancement, but only slight improvement of glutamic acid accumula- tion was observed ...odhA gene RBS sequence-substituted strain XW6 was selected for ...
11
A copper-controlled RNA interference system for reversible silencing of target genes in Trichoderma reesei
... The gene knock- out strategy by homologous recombination (HR) has been developed and widely used in ...target gene to ensure its occur- rence at an inherent low efficiency ...tmus53 gene of NHEJ ...
13
Metabolic regulation of Escherichia coli cultivated under anaerobic and aerobic conditions in response to the specific pathway gene knockouts
... the knockout of cra gene, and the carbon flow toward ca- tabolism and the glucose consumption rate are expected to increase, since glycolysis pathway genes such as ptsHI, pfkA and pykF are activated by the ...
14
Transcription Factor STE12α Has Distinct Roles in Morphogenesis, Virulence, and Ecological Fitness of the Primary Pathogenic Yeast Cryptococcus gattii
... ␣ gene was cloned by colony hybridization and sequenced using primer walk and 3 ⴕ rapid amplification of cDNA ends strategies, and a ste12 ␣⌬ gene knockout mutant was created by URA5 insertion at the ...
16
Butanol tolerance regulated by a two-component response regulator Slr1037 in photosynthetic Synechocystis sp. PCC 6803
... of gene knockout fragments [65]. Briefly, for the gene target selected, three sets of primers were designed to amplify a linear DNA fragment containing the chloram- phenicol resistance cassette ...
13
Automated pipeline for anatomical phenotyping of mouse embryos using micro CT
... single-gene knockout mice to gain an insight into gene ...these knockout mouse lines will be embryonic or perinatal ...the knockout, in comparison with the wild type, through a ...
9
Elucidation of the biosynthesis pathway and heterologous construction of a sustainable route for producing umbelliferone
... after gene knockout and overexpression, six strains were obtained to analyze their ability in producing Table 1 The candidate genes involved in umbelliferone ...
13
Tissue-Specific Gene Inactivation in Xenopus laevis: Knockout of lhx1 in the Kidney with CRISPR/Cas9
... tissue-targeted knockout strategy described in ...CRISPR/Cas9-mediated gene knockout can be targeted to the Xenopus kidney without perturbing essential early gene ...CRISPR gene editing ...
14
High-performance gene expression and knockout tools using sleeping beauty transposon system
... To clarify further the role of BRD7 in spindle orienta- tion and positioning, we analyzed the Z-stage stacks of the mitotic spindle by confocal microscopy and measured various parameters of the mitotic spindle (Fig. 6a ...
13
Original Article Tanshinone IIA protects against polyethylene particle-induced osteolysis response in a mouse calvarial model
... RANK gene knockout mouse, which proved that the RANKL gene expression in mice increased, and the bone absorption of osteo- clast induced by PE particles was observed in RANK+/+ but not RANKL-/- ...
11
Original Article Molecular cloning and stable knockout strain construction of the AcuE gene in Talaromyces marneffei
... An important feature of In-Fusion technology that makes it different from traditional DNA fusion cloning methods is the seamless con- nection, i.e., several segments can be fused to the backbone vector without any point ...
8
The neuronal migration hypothesis of dyslexia : a critical evaluation 30 years on
... Knockout mice for dyslexia susceptibility gene homologs KIAA0319 and KIAA0319L have unaffected neuronal migration but display abnormal auditory processing.. The neurological basis of dev[r] ...
49