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Gene Xpert MTB/RIF

Discordance across Several Methods for Drug Susceptibility Testing of Drug Resistant Mycobacterium tuberculosis Isolates in a Single Laboratory

Discordance across Several Methods for Drug Susceptibility Testing of Drug Resistant Mycobacterium tuberculosis Isolates in a Single Laboratory

... AST, Gene/Xpert MTB/ RIF, GenoType MTBDRplus line probe assay, MycoTB MIC plate, and a laboratory-developed mycobacteriophage quantitative PCR (qPCR)-based ...or RIF versus EMB or ...

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To Study the Comparative yield of Zn Staining v/s CBNAAT (Gene Xpert) in Clinically Diagnosed cases of Tubercular Pleural Effusion

To Study the Comparative yield of Zn Staining v/s CBNAAT (Gene Xpert) in Clinically Diagnosed cases of Tubercular Pleural Effusion

... that Gene Xpert MTB/RIF assay could play a significant role as routine diagnostic investigation in Clinico- Radiological & Biochemical suspected cases of tubercular pleural effusion and ...

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Ocular mycobacteriosis—dual infection of M. tuberculosis complex with M. fortuitum and M. bovis

Ocular mycobacteriosis—dual infection of M. tuberculosis complex with M. fortuitum and M. bovis

... Background: We report unfavorable outcome in a patient with subretinal granuloma caused by dual infection of Mycobacterium tuberculosis complex with Mycobacterium fortuitum and Mycobacterium bovis in an immunosuppressed, ...

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Rifampicin resistance mutations in the 81 bp RRDR of rpoB gene in Mycobacterium tuberculosis clinical isolates using Xpert MTB/RIF in Khyber Pakhtunkhwa, Pakistan: a retrospective study

Rifampicin resistance mutations in the 81 bp RRDR of rpoB gene in Mycobacterium tuberculosis clinical isolates using Xpert MTB/RIF in Khyber Pakhtunkhwa, Pakistan: a retrospective study

... % RIF resistant strains are also resistant to isoniazid [2, ...of Xpert MTB/RIF assay (Cepheid, USA) has revolutionized the diagnosis of TB by simultaneously detecting MTB and ...

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Comparison of Xpert MTB/RIF with Line Probe Assay for Detection of Rifampin Monoresistant Mycobacterium tuberculosis

Comparison of Xpert MTB/RIF with Line Probe Assay for Detection of Rifampin Monoresistant Mycobacterium tuberculosis

... the Xpert MTB/RIF and LPA in cases of RIF monoresistance compared to the gold standard MGIT960 culture-based DST, with further reconfirmation of these results with rpoB gene ...

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Distribution and Frequency of rpoB Mutations Detected by Xpert MTB/RIF Assay Among Beijing and Non-Beijing Rifampicin Resistant Mycobacterium tuberculosis Isolates in Bangladesh

<p>Distribution and Frequency of <em>rpo</em>B Mutations Detected by Xpert MTB/RIF Assay Among Beijing and Non-Beijing Rifampicin Resistant <em>Mycobacterium tuberculosis</em> Isolates in Bangladesh</p>

... rpoB gene which also encompasses the 81 bp RRDR region targeted by Xpert ...in Xpert assay was further evaluated by MTBDRplus assay, where sound punctual mutations were identi fi ed between ...rpoB ...

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Diagnostic Accuracy of IS6110 Insertion Gene, Hsp65, and Xpert MTB/RIF for Rapid  Diagnosis of Pulmonary Tuberculosis

Diagnostic Accuracy of IS6110 Insertion Gene, Hsp65, and Xpert MTB/RIF for Rapid Diagnosis of Pulmonary Tuberculosis

... Purpose: To evaluate diagnostic accuracy of IS 6110 insertion genes , hsp 65, and Xpert MTB/RIF for rapid diagnosis of pulmonary tuberculosis. Methods: Sixty patients, medically reported HIV ...

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Performance evaluation of the Xpert MTB/RIF assay according to its clinical application

Performance evaluation of the Xpert MTB/RIF assay according to its clinical application

... 104 MTB culture-positive samples, with 7 isolates ...to RIF. The Xpert assay correctly de- tected RIF resistance in 6 out of the 7 resistant ...the Xpert assay was ...for RIF ...

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Evaluation of the Analytical Performance of the Xpert MTB/RIF Assay

Evaluation of the Analytical Performance of the Xpert MTB/RIF Assay

... rpoB gene mutation 533ccg (ccg mutation at codon position 533) or 531ttg (ttg mutation at codon position 531) was performed at a final concentration of 90 copies per ...the MTB assay protocol, released on 8 ...

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Xpert MTB/RIF and Xpert MTB/RIF Ultra for pulmonary tuberculosis and rifampicin resistance in adults

Xpert MTB/RIF and Xpert MTB/RIF Ultra for pulmonary tuberculosis and rifampicin resistance in adults

... Xpert MTB/RIF uses molecular beacon technology to detect ri- fampicin ...rpoB gene of ...the gene sequences that is characteristic of rifampicin-susceptible tu- ...single Xpert ...

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The New Xpert MTB/RIF Ultra: Improving Detection of Mycobacterium tuberculosis and Resistance to Rifampin in an Assay Suitable for Point of Care Testing

The New Xpert MTB/RIF Ultra: Improving Detection of Mycobacterium tuberculosis and Resistance to Rifampin in an Assay Suitable for Point of Care Testing

... The Xpert MTB/RIF assay (Xpert) was devel- oped to improve TB and rifampin resistance (RIF-R) ...and RIF-R. Xpert uses a hemi-nested PCR to amplify the rifampin ...

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Laboratory Diagnosis of Mycobacterium tuberculosis Infection and Disease in Children

Laboratory Diagnosis of Mycobacterium tuberculosis Infection and Disease in Children

... NAAT, Xpert MTB/RIF (Cepheid, Sunnyvale, CA), has also been recommended as the initial diagnostic test for children suspected of having multidrug- resistant TB (MDR-TB) or HIV-associated TB in a 2013 ...

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Xpert® MTB/RIF assay for extrapulmonary tuberculosis and rifampicin resistance

Xpert® MTB/RIF assay for extrapulmonary tuberculosis and rifampicin resistance

... Xpert® MTB/RIF is a diagnostic test for detection of ...(NAATs), Xpert® MTB/ RIF integrates sample processing and PCR amplification and de- tection into a single self-enclosed ...

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Dried Culture Spots for Xpert MTB/RIF External Quality Assessment: Results of a Phase 1 Pilot Study in South Africa

Dried Culture Spots for Xpert MTB/RIF External Quality Assessment: Results of a Phase 1 Pilot Study in South Africa

... for Xpert MTB/RIF verification using different strains from different culture methods, the individual components are not unfamiliar to the field: filter paper has been used for the transportation and ...

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Evaluation of Xpert MTB/RIF and MODS assay for the diagnosis of pediatric tuberculosis

Evaluation of Xpert MTB/RIF and MODS assay for the diagnosis of pediatric tuberculosis

... TB Xpert performed well on gastric aspirates, with a similar relative sensitivity to liquid culture as for sputum samples ...and Xpert on sputum for clinically con- firmed cases is likely due to confounding ...

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Evaluation of the Xpert MTB/RIF test for the diagnosis of childhood pulmonary tuberculosis in Uganda: a cross sectional diagnostic study

Evaluation of the Xpert MTB/RIF test for the diagnosis of childhood pulmonary tuberculosis in Uganda: a cross sectional diagnostic study

... the Xpert MTB/ RIF ...the Xpert MTB/ RIF cartridges and sample reagents, we were unable to run the Xpert MTB/RIF test on the freshly collected sputum ...

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Same Day Tools, Including Xpert Ultra and IRISA TB, for Rapid Diagnosis of Pleural Tuberculosis: a Prospective Observational Study

Same Day Tools, Including Xpert Ultra and IRISA TB, for Rapid Diagnosis of Pleural Tuberculosis: a Prospective Observational Study

... including Xpert MTB/RIF Ultra (ULTRA), has hitherto not been comprehensively ...assay), Xpert MTB/RIF, and ULTRA performance outcomes were evaluated in pleural fluid samples from ...

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Diagnostic accuracy of Xpert MTB/RIF assay for musculoskeletal tuberculosis: a meta-analysis

Diagnostic accuracy of Xpert MTB/RIF assay for musculoskeletal tuberculosis: a meta-analysis

... TB, Xpert showed increased sensitivity and reduced specificity, which could be explained by rela- tively less positive and more negative results detected by the reference standard used in these ...

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Prevalence of pulmonary tuberculosis in patients with diabetes mellitus and lower respiratory tract infection

Prevalence of pulmonary tuberculosis in patients with diabetes mellitus and lower respiratory tract infection

... to Xpert assay which detects 131 bacilli / ...for MTB to grow due to the slow replication rate whereas Xpert assay provides result within two ...hours. Xpert assay result in these seven ...

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Diagnosis of paediatric tuberculosis by cartridge based nucleic acid amplification test and its effectiveness as compared to the other conventional diagnostic methods

Diagnosis of paediatric tuberculosis by cartridge based nucleic acid amplification test and its effectiveness as compared to the other conventional diagnostic methods

... A 200 µl portion of the deposit was resuspended in phosphate-buffered saline to a 500 µl volume. The sample reagent supplied with the test (1.5 ml) was then added. The mixture was vortexed for 30 s to ensure all bacteria ...

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