Genomic RNA
Sequence and translation of the murine coronavirus 5'-end genomic RNA reveals the N-terminal structure of the putative RNA polymerase.
... By comparing the translation products of virion genomic RNA and in vitro transcribed RNAs, we established that our clones encompassing the 5'-end mouse hepatitis virus genomic RNA encode[r] ...
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Functional cDNA clones of the human respiratory syncytial (RS) virus N, P, and L proteins support replication of RS virus genomic RNA analogs and define minimal trans-acting requirements for RNA replication.
... The RNA-dependent RNA polymerase of human respiratory syncytial (RS) virus was expressed in a func- tional form from a cDNA ...virus RNA entirely from cDNA ...virus genomic RNA analogs ...
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The connection domain in reverse transcriptase facilitates the in vivo annealing of tRNALys3 to HIV 1 genomic RNA
... dimensional polyacrylamide gel electrophoresis (1D PAGE). These results are shown in Figure 2A, and pre- sented graphically in Figure 2B. The left side of panel A shows that there is a linear increase in the reverse ...
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Mechanism of retroviral genomic RNA dimerization and packaging
... the genomic RNA, bind to flexible regions near the 3' end of PAL1 and PAL2 ...cause RNA conformational changes in the flexible domain, breaking the 5-bp helix and loosening the previously defined ...
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Studies of the Genomic RNA of Leukosis Viruses: Implications for RNA Dimerization
... stable RNA dimer that dissociates at a higher temperature (17, ...the RNA conforma- tional change since in vitro experiments show that HIV-1 NC is capable of converting the transient kissing-loop complex ...
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Nodavirus RNA Replication Protein A Induces Membrane Association of Genomic RNA
... FHV genomic RNA1 recruitment to membranes, implying that protein A interacts directly or indirectly (via host proteins) with FHV RNA1 to recruit it to a membrane-associated ...FHV genomic RNAs required both ...
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Preliminary Physical Mapping of RNA-RNA Linkages in the Genomic RNA of Moloney Murine Leukemia Virus
... on RNA function (12, 14, 25, ...the genomic RNA, facilitating its confinement in the interior of the ...viral RNA, facilitating the synthesis of full-length RNA by RNA polymerase ...
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RNA recombination in a coronavirus: recombination between viral genomic RNA and transfected RNA fragments.
... To rule out the possibility that the 864- RNA preparations were contaminated with genomic-sense RNA fragments or double-stranded DNA fragments, we examined cytoplasmic RNAs from L2 cells[r] ...
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Asynchronous Accumulation of Lettuce Infectious Yellows Virus RNAs 1 and 2 and Identification of an RNA 1 trans Enhancer of RNA 2 Accumulation
... LIYV genomic RNAs 1 and 2 is not synchro- nous. LIYV RNA 1 RNAs (both positive- and negative-sense genomic RNAs, as well as the RNA 1, ORF 2 [P32] sub- genomic RNA) were always ...
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Analysis of Nucleotide Sequences and Multimeric Forms of a Novel Satellite RNA Associated with Beet Black Scorch Virus
... single RNA was associated with infections, but a small single-stranded RNA (ssRNA) that is similar to the satellite RNAs (sat-RNA) of other necrovi- ruses (39) has been found in isolates from the ...
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Norovirus Genome Circularization and Efficient Replication Are Facilitated by Binding of PCBP2 and hnRNP A1
... MNV-1 genomic RNA. Two RNA molecules corresponding to the first 146 nt from the MNV-1 5= end and the complete 3= UTR without the poly(A) tail of the MNV-1 genomic RNA were synthesized ...
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cis-acting sequences required for encapsidation of duck hepatitis B virus pregenomic RNA.
... b Cells were transfected with WT DHBV DNA, D3-0X118 DNA, or both, and the genomic RNA present in equal portions of preparations of total cellular polyA+ RNA and encapsidated RNA were qua[r] ...
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Absolute quantitation of viremia in human immunodeficiency virus infection by competitive reverse transcription and polymerase chain reaction
... Furthermore, the reliability of the method observed in the precise quantification of wild-type DNA and RNA sequences which was checked by using plasmid molecules and genomic RNA; the lev[r] ...
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Selective and Nonselective Packaging of Cellular RNAs in Retrovirus Particles
... viral genomic RNA. However, in the absence of packageable viral RNA, assembly is still efficient, and the released virus-like particles (termed “ ⌿ ⴚ ” particles) still contain roughly normal amounts ...
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Generation of infectious RNA complexes in orbiviruses: RNA- RNA interactions of genomic segments
... segmented RNA genomes must package the correct number of segments for synthesis of infectious virus ...double-stranded RNA genomes achieve this challenging task by forming RNA networks of segments ...
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RNA-Templated Replication of Hepatitis Delta Virus: Genomic and Antigenomic RNAs Associate with Different Nuclear Bodies
... Western blotting. Western blotting was performed as previously described (26). Immunoblotting was carried out using anti-HDAg (1:200) (3G3), anti-Pol II (1:200) (Abcam), anti-SL1 (1:2500) (a generous gift from Lucio ...
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Construction of rubella virus genome-length cDNA clones and synthesis of infectious RNA transcripts.
... In the communication in which the sequence of the RUB genomic RNA was reported 7, dideoxy sequencing using polyA+ RNA extracted from RUB-infected cells as a template and oligonucleotide [r] ...
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Rapid and simple PCR assay for quantitation of human immunodeficiency virus type 1 RNA in plasma: application to acute retroviral infection
... A dilution series between 20 and 120 copies of the pCC2 HIV-1 transcript RNA 0, the pNAS2 quantitation standard transcript RNA [1 and HIV-1 genomic RNA A was each amplified for 26, 27, a[r] ...
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Detection of vesicular stomatitis virus RNA and its defective-interfering particles in individual mouse brains.
... For differentiation of hybridizable sequences found in the RNA of DI particles from complementary or identical sequences in the L mRNA or standard genomic RNA of VSV, RNA obtained from m[r] ...
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Detection of group B rotavirus in fecal specimens by dot hybridization with a cloned cDNA probe
... Northern blot hybridizations of bovine GBR genomic RNA with 32P-labeled probes synthesized from either whole bovine GBR genomic RNA left or the cDNA clone of gene 3 of the IDIR strain of[r] ...
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