Genotyping the DNA samples
Pedigree and genotyping quality analyses of over 10,000 DNA samples from the Generation Scotland : Scottish Family Health Study
... GS:SFHS DNA samples will soon be gen- erated, together with whole exome sequencing of DNA from nearly 1,000 ...scale genotyping or DNA sequencing, family relationships are verified ...
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Saliva samples are a viable alternative to blood samples as a source of DNA for high throughput genotyping
... Saliva DNA samples were plated out in dupli- cate on a 384 well plate, alongside additional duplicate DNAs taken from the paired blood samples (all normal- ised to 2 ...
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Detection of HPV DNA in paraffin embedded cervical samples: a comparison of four genotyping methods
... based genotyping may be worse in less experienced and less rigorous laboratories, as indicated by a WHO HPV laboratory comparison study including 29 centers across the world, which reported lower proficiency ...
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Forensic Genotyping as a Method To Teach Genetics & DNA Science
... Next, samples are loaded into the genetic analyzer, which are auto- matically injected, electrophoresed, and analyzed ...personal DNA samples and the crime scene samples, and enters ...
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A METHODOLOGICAL COMPARISON AMONG DNA SOURCE TYPES FOR MOOSE GENOTYPING
... quantity DNA in fecal samples is the increased risk of genotyping errors such as allelic dropout or false ...estimate genotyping error, PCR and allele scoring was repeated for at least 24 ...
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Saliva DNA quality and genotyping efficiency in a predominantly elderly population
... of DNA from blood and saliva sam- ples were determined using the fluorescence-based Quant iT dsDNA Board-Range (BR) assay kit (cat # Q- 33130, Life Technologies, ...methods. DNA quantification by UV ...
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Saliva samples are a viable alternative to blood samples as a source of DNA for high throughput genotyping
... Oragene DNA Self-Collection kit (DNA Genotek W ) together with the manufacturer ’s ...the samples by post or in person, over a period of 2 months from April 2008 to May ...2008. Samples were ...
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Performance of a Polymer Based DNA Chip Platform in Detection and Genotyping of Human Papillomavirus in Clinical Samples
... other samples can be handled consecutively by using seal frames for sample hybrid- ...12 samples can be analyzed within 3 ...genotype-specific DNA probes immobilized on a nitro- cellulose strip, ...
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Genotyping DNA pools on microarrays: tackling the QTL problem of large samples and large numbers of SNPs
... between DNA pools of the same group of individuals and compared allele frequency esti- mates for pooled DNA from this group of individuals to individual ...pooled DNA estimates are not compared ...
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Comparison of eleven methods for genomic DNA extraction suitable for large-scale whole-genome genotyping and long-term DNA banking using blood samples
... genomic DNA is prerequisite for successful and reliable large scale genotyping ...pure DNA are also required for the creation of ...different DNA extraction procedures, including ...
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Discovery and genotyping of DNA sequence variations in grape.
... 2.4 Discussion The genetic diversity of 62 grape accessions including 38 autochthonous grapevines from Campania was investigated using SSR and retrotransposon-based molecular markers. The gene pool of cultivated grapes ...
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Genotyping of JC Virus in Clinical Samples in North Eastern Iran
... the DNA extraction. We used Yektatajhiz Azma kits for DNA extraction from specimens and urine samples according to the kits ...extracted DNA was measured using a nanodrop and samples ...
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Enhanced Detection and Typing of Human Papillomavirus (HPV) DNA in Anogenital Samples with PGMY Primers and the Linear Array HPV Genotyping Test
... when samples positive for HPV-16 with LA-HPV only were further investigated with a real-time PCR assay, most were confirmed as positive for HPV-16 ...same samples confirmed the results ob- tained with ...
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Saliva samples as a source of DNA for high throughput genotyping: an acceptable and sufficient means in improvement of risk estimation throughout mammographic diagnostics
... bacterial DNA con- tent, we performed a PCR amplification using primer sets specific for human beta-globin [37] or bacterial 16s ...human DNA but is conserved across a large range of microorganisms ...
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Isolation and Genotyping of Acanthamoeba from Soil Samples in Markazi Province, Iran
... A Master Mix Kit (CinnaGen Co.) was used to optimise the PCR reactions. A final volume of 50 µL was used for amplification in an Eppendorf thermocycler with incubation at 95°C for 3 minutes, followed by 35 cycles at 94°C ...
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Discovery and genotyping of existing and induced DNA sequence variation in potato
... of genotyping- by-sequencing (GBS) in a large collection of autotetraploid potato cultivars using next- generation ...12 samples using the Illumina ...tetraploid samples in the potato ...SNP ...
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A Novel and Rapid PCR Based Method for Genotyping Human Papillomaviruses in Clinical Samples
... three samples could be genotyped. Samples 1 and 3 were found to contain HPV-16; sample 2 was found to contain ...new genotyping method to the analysis of large numbers of clinical samples ...
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Isolation of Plasmodium falciparum by flow cytometry: implications for single trophozoite genotyping and parasite DNA purification for whole genome high throughput sequencing of archival samples
... cell genotyping opens new possibilities for the characterisation of the different haplotypes present within ...for genotyping or sequencing are improving so rapidly it is likely that better yields could be ...
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Quality, quantity and recovery of DNA content from routine blood samples and genotyping success rate: comparison between phenol chloroform method (PCM) with a new kit-based DNA extraction method (KBM)
... of genotyping as shown by amplification plot or allelic discrimination demonstrated more success in KBM (98% ...increased DNA concentration exposure to ...of DNA can lead to non-specific binding of ...
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Original Article STR DNA genotyping of hydatidiform moles in South China
... Interphase FISH can be used for the determina- tion of the number of haploid chromosome sets using both fresh and FFPE tissue samples. But, similar to ploidy analysis, it cannot distinguish a diploid complete mole ...
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