Geobacillus stearothermophilus
Strain Improvement of Bacillus coagulans and Geobacillus stearothermophilus for Enhanced Thermostable Cellulase Production and the Effect of Different Metal Ions on Cellulase Activity
... The current study was focused on the strain improvement of Bacillus coagulans and Geobacillus stearothermophilus for thermostable cellulase production with higher enzyme activity. For strain improvement UV ...
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Structural basis of substrate binding in WsaF, a rhamnosyltransferase from Geobacillus stearothermophilus
... Carbohydrate polymers are medically and industrially important. The S-layer of many Gram-positive organisms comprises protein and carbohydrate polymers and forms an almost paracrystalline array on the cell surface. Not ...
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Characterisation of dairy strains of Geobacillus stearothermophilus and a genomics insight into its growth and survival during dairy manufacture : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Microbiology at Massey University, Palmerston North, New Zealand
... as Geobacillus stearothermophilus and Anoxybacillus flavithermus, which tend to grow to high numbers in milk powder manufacturing plants (Scott et ...recently Geobacillus glucosidasius has also been ...
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Purification and characterization of an acidic, thermophilic phytase from a newly isolated Geobacillus stearothermophilus strain DM12
... increased up to 48 h of incubation and then gradually decreased up to 40% at 96 h. So, the highest phytase production was observed after 2 days of incubation. This strain also lowered the pH (from initial pH 6.8 to 4.0) ...
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Application of the thermostable β-galactosidase, BgaB, from Geobacillus stearothermophilus as a versatile reporter under anaerobic and aerobic conditions
... Use of thermophilic organisms has a range of advantages, but the significant lack of engineering tools limits their applications. Here we show that β-galactosidase from Geobacillus stearothermophilus (BgaB) ...
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Phototriggering Nitric Oxide Synthase from Geobacillus stearothermophilus
... The reaction proceeds in two turnovers with N -hydroxy-Larginine NOHA as a stable bound intermediate.3 All mNOS isoforms are homodimers that contain an oxygenase domain linked to a reduc[r] ...
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Characterisation of dairy strains of Geobacillus stearothermophilus and a genomics insight into its growth and survival during dairy manufacture : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Microbiology at Massey University, Palmerston North, New Zealand
... spectrometry and fatty profiling demonstrated that the population was diverse. In Chapter 3, it was shown that the same ten strains varied in their ability to form biofilms and produce spores. Three strains of G. ...
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Preliminary characterization of the probiotic properties of Candida famata and Geobacillus thermoleovorans
... of the growth medium pH or the volatile compounds production (14). In this study, the inhibitory mechanism of the interaction was not characterized, but several previously researches reported that Bacillus produces ...
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Material Conformity and Bactericidal Characteristics of High Density Ozone Disinfection in Vinyl Polysiloxane Impression and PMMA Dentistry Fillings
... and Geobacillus stearothermophilus (formerly Bacillus stearothermophilus); a dental yeast-like fungus, Candida albicans and selected species of pathogenic bacteria, Aspergillus niger, Pseudomonas ...
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The Impact of Education Decentralization on Education Output: A Cross Country Study
... INDEX WORDS: thermostable carboxylesterase, Geobacillus stearothermophilus, crystal structure, multiple anomalous dispersion, alpha/beta hydrolase, tetrahedral intermediate, reaction mec[r] ...
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A novel method for transforming the thermophilic bacterium Geobacillus kaustophilus
... genus Geobacillus was first described in 2001 ...modify Geobacillus species are available ...for Geobacillus stearothermophilus NUB36 [14] and an electroporation ...
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In vivo selection of sfGFP variants with improved and reliable functionality in industrially important thermophilic bacteria
... in different bacterial expression hosts (Table 2). The FP genes were inserted into the multiple cloning site of a derivative of the E. coli–Bacillus shuttle vector pNW33N (Additional file 2), thereby enabling a ...
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Development of a Genetic Toolbox for Geobacillus kaustophilus using Novel Bacteriophages GBK1 and GBK2.
... as Geobacillus species are mixed acid ...for Geobacillus species in the form of potential promoters, DNA modifying enzymes, auxotrophic complementation systems, and the development of plasmids capable of ...
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Effect of the Extracts from Leaves of Acanthus Montanus (Nees) T. Anders. (Acanthaceae) on Germination and Outgrowth of Some Bacillus Spores
... After heat activation at 80°C during 10 min, spores were exposed to the crude extract and ethyl acetate fraction at the concentration of 1 mg/ml and 0,5 mg/ml respectively follow by water wash. To allow them to germinate ...
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Complete genome sequences of Geobacillus sp. WCH70, a thermophilic strain isolated from wood compost
... Bacillus, Geobacillus species were reclassified into a separate genus based on properties such as 16S rRNA gene se- quence analysis, lipid and fatty acid analysis, phenotypic characterization, and DNA–DNA ...
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STUDY OF ENHANCED LIPASE PRODUCTION USING AGROWASTE PRODUCT BY BACILLUS STEAROTHERMOPHILUS MTCC 37
... The important energy sources that express for the higher production of lipase activity are carbon and nitrogen sources under submerged fermentation conditions has [r] ...
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Novel Geobacillus thermoleovorans KNG 112 Thermophilic Bacteria from Bandaru Hot Spring: A Potential Producer of Thermostable enzymes
... Results: A Gram-positive, aerobic, and non-motile Geobacillus sp. were isolated and characterized from a hot spring of Karnataka. Various biochemical tests for the strain IC5 revealed that the bacteria are capable ...
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Thermophilic fermentation of acetoin and 2,3-butanediol by a novel Geobacillus strain
... Compared with other thermophilic bacteria, the ability of degradation and utilization of hydrocarbons at high temperature is another important feature of Geobacillus species [16]. After one week of aerobic ...
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Heterologous Expression and Characterization of Thermostable Lipases from Geobacillus Thermoleovorans PPD2 Through Escherichia Coli
... Heterologous expression and purification of thermostable lipase from Geobacillus thermoleovorans PPD2 had been carried out through Escherichia coli BL21 as host. Two bands obtained showed lipolytic activity with ...
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In silico analysis and truncation studies of the c terminal alpha amylase from anoxybacillus species
... Raw starch degrading enzymes are mainly belonging to glycoside hydrolases (GH) 13, 14 and 15, which cleave glycosidic bonds either from the non-reducing end or in the interior of starch chemical structure (Puspasari et ...
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