GFP-fusion protein
Structural stability and endonuclease activity of a PI-SceI GFP-fusion protein
... PI-SceI GFP-fusion protein structure, SDS-PAGE of purified proteins, and illustration of PCR steps performed to create and amplify the PI-SceI-GFP encoding ...of GFP and PI-SceI intein ...
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Subcellular Localization of Transmembrane E cadherin GFP Fusion Protein and Expression in HeLa Cells
... Fluorescent Protein (GFP) was derived from the jellyfish Aequorea victoria (yellow tissue masses comprising about 6000-7000 photogenic cells working with the enzyme aequorin) by Osamu Shimomura in ...the ...
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GFP Fusion Proteins: A Solution or a Problem?
... a GFP fusion ...YB-1-GFP fusion GFP exogenous expression. Transfect- ed YB-1-GFP was highly expressed inHEK293T cells (Figure ...
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Engrailed homeoprotein acts as a signaling molecule in the developing fly
... En protein is also present in the extracellular space, we performed detergent-free immunocytochemistry on wing discs (Furrer et ...En-GFP fusion protein in the larval eye disc, which has ...
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Role of Ferroxidases in Iron Uptake and Virulence of Cryptococcus neoformans
... Cfo1-GFP fusion protein in the pka1 ...homolog protein Fet3 in ...Cfo1-GFP fusion protein in the pka1 mutant (data not ...Cfo1 protein and may also contribute to ...
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Rivoflavin may interfere with on-line monitoring of secreted green fluorescence protein fusion proteins in Pichia pastoris
... GFP fusion protein fluorescence in culture supernatant and cells samples were measured using a Perkin Elmer LS55 Spectrophotometer (PerkinElmer ...intracellular GFP and cellular ...
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Role for Hsp70 Chaperone in Saccharomyces cerevisiae Prion Seed Replication
... the GFP insertion did not sig- nificantly affect prion interactions of the N domains of NGMC ...Sup35-GFP fusion protein functions ...Sup35-GFP fusion has GFP inserted ...
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Regulation of Physiological Rates in Caenorhabditis elegans by a tRNA-Modifying Enzyme in the Mitochondria
... GRO-1::GFP fusion protein is localized diffusely throughout the cytoplasm and ...a protein initiated from the second methionine is localized diffusely throughout the cell and does not rescue ...
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ATP-Dependent Localization of the Herpes Simplex Virus Capsid Protein VP26 to Sites of Procapsid Maturation
... VP26-GFP fusion protein showed similar ATP-dependent relocalization, and al- though a VP26-GFP fusion similar to this one is known to be incorporated normally into infectious HSV ...
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Farnesyl diphosphate synthase, the target for nitrogen-containing bisphosphonate drugs, is a peroxisomal enzyme in the model system Dictyostelium discoideum
... FDPS–GFP fusion protein was expressed in mammalian cells but it was not possible to determine whether it was located in the peroxisomes or in the cytosol ...
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Spectral properties of LH2 exhibit very similar even when heterologously express LH2 with β subunit fusion protein in Rhodobacter sphaeroides
... purified as described in our previous literatures, respec- tively [28,29]. Rb. sphaeroides cells were harvested and resuspended with resuspension buffer (20 mM Tris-HCl, pH 8.0, 100 mM NaCl). DNase I and RNase A were ...
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Two Dictyostelium Orthologs of the Prokaryotic Cell Division Protein FtsZ Localize to Mitochondria and Are Required for the Maintenance of Normal Mitochondrial Morphology
... FszA-GFP fusion protein (as judged by the intensity of GFP fluorescence), the protein formed belts and puncta similar to those observed by immu- nofluorescence ...of protein, ...
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Design, characterization and in vivo functioning of a light-dependent histidine protein kinase in the yeast Saccharomyces cerevisiae
... regulator protein had been genetically deleted and replaced by the C1 hybrid kinase and a translational HOG1::GFP fusion protein, ...their GFP- and DAPI content, respectively, we then ...
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Human Cytomegalovirus UL97 Kinase Activity Is Required for the Hyperphosphorylation of Retinoblastoma Protein and Inhibits the Formation of Nuclear Aggresomes
... by using a pp65-GFP fusion protein, and a series of cotrans- fection experiments implicated both pp71 and IE1 as affecting aggresome formation. This observation was fortuitous, because the functions ...
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Evaluation of VP22 Spread in Tissue Culture
... the GFP fusion ...affecting GFP fluo- rescence of the fusion protein, this might be directly demon- strated by testing the ability of PFA to quench the intrinsic GFP fluorescence ...
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The Hinge of the Human Papillomavirus Type 11 E2 Protein Contains Major Determinants for Nuclear Localization and Nuclear Matrix Association
... We have no information on the focal components to which the N domain associates, but there are a number of potential candidates. For instance, our recent immunofluorescence stud- ies of HPV replication compartments in ...
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Nested Genes CDA12 and CDA13 Encode Proteins Associated with Membrane Trafficking in the Ciliate Tetrahymena thermophila
... cell fusion product showing multiple micronuclei (pink), as well as macronuclear and cytoplasmic fission ...exconjugant fusion mass exhibiting the effects of numerous rounds of cytokinetic fission failure ...
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Intracellular Localization of ABC Transporter TAPL Differs between Transient and Stable Expression
... marker protein, cathepsin D, rather than an endoplasmic reticulum marker, ...with GFP under transient expression could become targets of quality con- trol system(s) of ER [20] and that the exit of TAPL ...
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The Fission Yeast Schizosaccharomyces pombe Has Two Importin-α Proteins, Imp1p and Cut15p, Which Have Common and Unique Functions in Nucleocytoplasmic Transport and Cell Cycle Progression
... generous gift of Minoru Yoshida) was integrated at the leu1 locus by linearizing the DNA using NotI and transforming it into wild-type haploid cells (SS959). The imp1D mutant was constructed by replacing the open reading ...
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Co-transfection of plasmid DNA and laser-generated gold nanoparticles does not disturb the bioactivity of GFP-HMGB1 fusion protein
... observed GFP fluorescence of the respective fusion proteins was so intense that it even leaked into the DAPI channel (Fig 2M and 2N respectively for AuNP of d h = 59 ...recombinant protein ...
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