Glucosylceramide synthase (GCS)
Altered methylation of glucosylceramide synthase promoter regulates its expression and associates with acquired multidrug resistance in invasive ductal breast cancer
... of glucosylceramide synthase (GCS) increases multidrug resistance (MDR) in many cancer ...the GCS gene promoter with its expression and MDR in invasive ductal breast ...cases ...
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An increase in glucosylceramide synthase induces Bcl- xL-mediated cell survival in vinorelbine-resistant lung adenocarcinoma cells
... Inhibiting GCS with PDMP all significantly (P < ...of glucosylceramide because the sphingolipid metabolites are typically regulated during ceramide ...to glucosylceramide, sphingosine-1-phosphate, ...
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Efficacy of Enzyme and Substrate Reduction Therapy with a Novel Antagonist of Glucosylceramide Synthase for Fabry Disease
... inhibiting glucosylceramide synthase (GCS) were ...available GCS inhibitor (Genz-682452) with pharmacological and safety profiles that have poten- tial for treating Fabry ...
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Inhibition of glucosylceramide synthase eliminates the oncogenic function of p53 R273H mutant in the epithelial-mesenchymal transition and induced pluripotency of colon cancer cells
... Western blotting was carried out as described previously [33, 54]. Briefly, cells or tissue homogenates were lysed in NP40 cell lysis buffer (Biosource, Camarillo, CA, USA) to extract total cellular proteins once the ...
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Expression of glucosylceramide synthase in invasive ductal breast cancer may be correlated with high estrogen receptor status and low HER-2 status
... of GCS, an enzyme that glycosylates ceramide and inhibits its proapoptotic activity in ...the GCS gene in the drug-resistant human breast cancer cell line MCF-7/ADM is higher than that in drug sensitive ...
7
Reducing Glycosphingolipid Content in Adipose Tissue of Obese Mice Restores Insulin Sensitivity, Adipogenesis and Reduces Inflammation
... of glucosylceramide synthase (GCS) activity with N-(5-adamantane-1-yl-methoxy)- pentyl-1-deoxynojirimycin (AMP-DNM) corrects insulin resistance in cultured adipocytes from obese individuals and 3T3 ...
11
Glucosylceramide synthase is an essential regulator of pathogenicity of Cryptococcus neoformans
... The pathogenic fungus Cryptococcus neoformans infects humans upon inhalation and causes the most common fungal meningoencephalitis in immunocompromised subjects worldwide. In the host, C. neoformans is found both ...
10
Lipophilic iminosugars : synthesis and evaluation as inhibitors of glucosylceramide metabolism
... in glucosylceramide metabolism. Glucosylceramide, a β-glycoside of the lipid ceramide and the carbohydrate -glucose, is a key member of a class of biomolecules called the glycosphingolipids ...enzyme, ...
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Iminosugars as glucosylceramide processing enzymes inhibitors: design, synthesis and evaluation
... in glucosylceramide and (partial) deficiency in GBA1 leads to accumulation of this substrate as well as the unusual lysolipid, ...disease, glucosylceramide synthase (GCS, producing ...
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Adipocytes harbor a glucosylceramide biosynthesis pathway involved in iNKT cell activation
... Unraveling Ugcg function may be key to untangling the metabolic effects of iNKT cells, ceramide and glucosylceramides, which appear to be intertwined. Ugcg is considered the only genomic glucosylceramide ...
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Reduction of globotriaosylceramide in Fabry disease mice by substrate deprivation
... The increase in cell ceramide levels was interpreted to be the result of substrate accumulation resulting from glucosylceramide synthase inhibition. However, with the synthesis and evaluation of various ...
10
Targeting ceramide synthase 6–dependent metastasis prone phenotype in lung cancer cells
... migration. Sphingolipids are often altered in cancer, though how these alterations lead to tumor formation and progression is largely unknown. Here, we analyzed non–small-cell lung cancer (NSCLC) specimens and cell lines ...
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Biosynthesis and Immunogenicity of Glucosylceramide in Cryptococcus neoformans and Other Human Pathogens
... Also, the fatty acid substituents in the GlcCer are long-chain fatty acids (LCFA), usually between 16 and 18 carbons long (93–95, 100) (Fig. 3). This is in contrast to the 24- to 26-carbon chain length present in the ...
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The therapeutic potential of modulating the ceramide/sphingomyelin pathway
... as glucosylceramide synthase inhibitors, which prevent the siphoning off of ceramide for gly- cosphingolipid synthesis, or safingol ( L -threo-dihy- drosphingosine), a sphingosine kinase inhibitor that pre- ...
7
Consequences of beta glucocerebrosidase deficiency in epidermis Ultrastructure and permeability barrier alterations in Gaucher disease
... electron-dense tracer, colloidal lanthanum, percolated between the incompletely processed lamellar body-derived sheets in the SC interstices of Gaucher mice only, demonstrating altered permeability barrier function. ...
10
Graphene oxide-reinforced biodegradable genipin-cross-linked chitosan fluorescent biocomposite film and its cytocompatibility
... the GCS/GO films were significantly increased with the increasing load of ...the GCS/GO composite film also maintained the intrinsic fluorescence of ...The GCS/GO biocomposite films might have a ...
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Virulence-Associated Enzymes of Cryptococcus neoformans
... Definition of enzymatic pathways can provide crucial targets for antimicrobial drug design. One way to identify targets is to iden- tify unique metabolic requirements for cryptococcal growth and/or virulence. An example ...
13
Risk Factors for Positive CT in Head Injured Patients with Admission GCS 15
... trauma patients with mild alterations in consciousness (GCS 13-14), reported an incidence of 27.4% abnormal CT scans and 3.7% required surgical intervention in a selected group of patients and concluded that great ...
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Squalene synthase cloning and functional identification in wintersweet plant (Chimonanthus zhejiangensis)
... Cloning of squalene synthase genes from wintersweet The transcriptome of the C. zhejiangensis foliage was sequenced by Illumina. The data was assembled by the Trinity software (Grabherr et al. 2011) and the result ...
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Investigations of carbocyclisation reactions in prodiginine alkaloid biosynthesis
... Analysis of cluster 15 identified by antiSMASH, which shows 53% similarity to the gene cluster that directs the biosynthesis of ECO-02301, suggested it directs the production of a novel metabolite (Figure 4.8). ECO-02301 ...
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