Glutamine synthetase
Glutamine accumulation and up regulation of glutamine synthetase activity in the swamp eel, Monopterus albus (Zuiew), exposed to brackish water
... Increased glutamine synthesis is a defence mechanism commonly found in the brains of vertebrates (Cooper and Plum, 1987), including fish (Ip et ...of glutamine synthetase ...increase glutamine ...
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Induction of four glutamine synthetase genes in brain of rainbow trout in response to elevated environmental ammonia
... Primers and dual-labeled probes (Table·1) were designed for each gene using PrimerExpress software (v. 2.0, Applied Biosystems). All probes were dual-labeled with 6-FAM fluorescent reporter at the 3 ⬘ end and TAMRA ...
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Interaction of Glufosinate and Colletotrichum truncatum on Ammonia Levels and Glutamine Synthetase Activity in Hemp Sesbania
... 4.2. Glutamine Synthetase Assay and Localization on PAGE Gels C. truncatum treatment of hemp sesbania did not cause significant effects on GS activity until after 40 h, a time when hypocotyls lose their ...
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Induction of carbamoyl phosphate synthetase III and glutamine synthetase mRNA during confinement stress in gulf toadfish (Opsanus beta)
... hepatic glutamine synthetase (GSase) activity (Walsh et ...of glutamine- dependent ureotelism in toadfish (Anderson and Walsh, 1995; Walsh and Milligan, ...
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A second glutamine synthetase gene with expression in the gills of the gulf toadfish (Opsanus beta)
... Western blots (Fig.·1) of SDS gels demonstrated that GSases in all tissues except muscle appear as a single subunit band with a molecular mass of approximately 49.4·kDa. This value differs slightly from the 42.3–42.4·kDa ...
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The marble goby oxyeleotris marmoratus activates hepatic glutamine synthetase and detoxifies ammonia to glutamine during air exposure
... to glutamine (Levi et ...hepatic glutamine synthetase activity was found to be significantly different from control ...pre-existing glutamine synthetase is sufficient to account for the ...
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Urea synthesis in the African lungfish Protopterus dolloi hepatic carbamoyl phosphate synthetase III and glutamine synthetase are upregulated by 6 days of aerial exposure
... total free amino acid content. This indirectly confirms that the specimen had reduced its rates of proteolysis and/or amino acid catabolism to suppress endogenous ammonia production. Simultaneously, there were ...
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DOCKING STUDIES ON ANTIMICROBIAL PEPTIDES RELATED TO APIDAECIN IA AND HUMAN HISTATIN AGAINST GLUTAMINE SYNTHETASE AND RNA POLYMERASE IN MYCOBACTERIUM TUBERCULOSIS
... patients. Glutamine synthetase [1] produced the condensation reaction along with ...of glutamine and hydrolysis of adenosine ...signals. Glutamine synthetase can be inhibited by using ...
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Evolution of glutamine synthetase in vertebrates: multiple glutamine synthetase genes expressed in rainbow trout (Oncorhynchus mykiss)
... The function of multiple GSase genes in some bony fishes is intriguing. The high degree of conservation of GSase genes throughout eukaryote and prokaryote evolution (Pesole et al., 1991), and the lack of variation noted ...
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The freshwater Amazonian stingray, Potamotrygon motoro, up regulates glutamine synthetase activity and protein abundance, and accumulates glutamine when exposed to brackish (15‰) water
... up-regulate glutamine synthetase (GS) activity and protein abundance, and accumulate glutamine during a progressive transfer from freshwater to brackish (15‰) water with daily ...glutamate, ...
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Nitrogen compounds and enzyme activities in sorghum induced to water deficit during three stages
... these results on proteins were promoted by two factors, i.e. the increase in proteolysis, be- cause these enzymes breakdown the proteins, and a decrease of the protein synthesis due the water deficit. the maximum values ...
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Characterization of nitrogen metabolism and photosynthesis in a stay-green rice cultivar
... plants. Glutamine synthetase is a key enzyme of ammonia assimilation, plays an essential role in the transfor- mation from inorganic N into organic N (Xu and Zhou ...Additionally, glutamine ...
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Zinc fertilization alters flour protein composition of winter wheat genotypes varying in gluten content
... Experimental design. Each plastic pot (diameter 25 cm, height 30 cm) contained 10 kg of soil. Urea CO(NH 2 ) 2 , Ca(H 2 PO 4 ) and KCl were applied in each treatment at the rate of 90 mg N, 90 mg P and 75 mg K per kg ...
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Ammonia and urea transporters in gills of fish and aquatic crustaceans
... enzyme glutamine synthetase (Walsh et ...provide glutamine as the nitrogen donor for the CPSase III-based urea excretion in the liver and muscle of this ...on glutamine to produce ammonia may ...
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Pathways of inorganic nitrogen assimilation in chemoautotrophic bacteria marine invertebrate symbioses: expression of host and symbiont glutamine synthetase
... Activities of inorganic nitrogen assimilation enzymes The activities of glutamine synthetase (GS), glutamate dehydrogenase (GDH) and nitrate reductase (NR) were measured in several chemoautotrophic ...
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Structure and expression of glutamine synthetase in root nodules of Phaseolus Vulgaris L
... lowering the oxygen tension. However, studies on pea root expression during microaerobic growth conditions only detected an increase in alcohol dehydrogenase mRNA abundance (Govers et al, 1986). Haem has been observed to ...
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Urea transporter and glutamine synthetase regulation and localization in gulf toadfish gill
... to determine the location of LGS, probably due to its markedly lower transcript abundance as demonstrated by qPCR measurements. Similar to GGS, and in contrast to speculation by Laurent and colleagues (Laurent et al., ...
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Effects of crowding on ornithine–urea cycle enzyme mRNA expression and activity in gulf toadfish (Opsanus beta)
... Liver glutamine synthetase (GS), carbamoyl phosphate synthetase III (CPS), ornithine carbamoyl transferase (OCT) and arginase (ARG) activities were also ...argininosuccinate synthetase (ASS) ...
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Adjustments of nitrogen and carbon metabolisms were the mainly strategy followed in ammonium fed tomato to alleviate cadmium toxicity
... In order to investigate the influence of cadmium on nitrogen management, we measured activity and proteins contents for glutamine synthetase (GS). The presence of Cd in the culture medium resulted in a ...
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Exposure to air, but not seawater, increases the glutamine content and the glutamine synthetase activity in the marsh clam Polymesoda expansa
... of glutamine synthesis, similar to the adoption of urea synthesis, for ammonia detoxification first took place among ...to glutamine during emersion. The capacity for glutamine synthesis was present ...
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