Glutathione synthesis
"Shock and kill" effects of class I selective histone deacetylase inhibitors in combination with the glutathione synthesis inhibitor buthionine sulfoximine in cell line models for HIV 1 quiescence
... BSO, at concentrations of up to 500 μM, did not signifi- cantly raise the p24 concentrations; yet it increased the HIV-1 promoting effects of class I HDACIs, such as MS- 275 (Figure 4A) and MC2113 (data not shown) in ...
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PKLR promotes colorectal cancer liver colonization through induction of glutathione synthesis
... increasing glutathione, the primary endogenous ...cellular glutathione levels. Glutathione is critical for metastasis, and we determined that the rate-limiting enzyme of glutathione ...
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Hemin Protects U937 Cells from Oxidative Stress via Glutathione Synthesis
... One of the potential master regulators that control antioxidative machineries is a transcription factor, nuclear factor-erythroid-2-related factor (Nrf-2). It has previously been demonstrated that the HO-1 gene and GCLC ...
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Effects of cadmium on cellular protein and glutathione synthesis and expression of stress proteins in eastern oysters, Crassostrea virginica Gmelin
... the synthesis of cellular protective proteins and ...and glutathione (GSH) synthesis and expression of stress proteins (heat shock proteins HSP60, HSP70 and HSP90) and metallothioneins in isolated ...
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Burn injury differentially alters whole-blood and organ glutathione synthesis rates: An experimental model
... cysteine and GSH was determined by gas chromatography/mass spectrometry. Plasma cysteine metabolic fl ux was increased signifi cantly (P < 0.01) following burn injury. WB, liver, and lung GSH concentrations (P = ...
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Glutathione biosynthesis in human erythrocytes: I Identification of the enzymes of glutathione synthesis in hemolysates
... erythrocytes. Glutamyl cysteine synthetase requires glutamic acid, cysteine, adenosine triphosphate (ATP), and magnesium ions to form g-glutamyl cysteine. The activity of this enzyme in hemolysates from 25 normal ...
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Glutathione Synthesis in Human Erythrocytes: II PURIFICATION AND PROPERTIES OF THE ENZYMES OF GLUTATHIONE BIOSYNTHESIS
... GSH synthetase, purified through the second ammonium sulfate step, was diluted to 3 mg protein/ml in 0.02 M potassium phosphate pH 7.0 containing 0.005 M dithiothreitol and 0.03 M GSH wh[r] ...
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Inhibition of glutathione synthesis augments lysis of murine tumor cells by sulfhydryl reactive antineoplastics
... (vernolepin, helenalin, elephantopin, and eriofertopin) for 1 h resulted in 70-97% depletion of GSH. The importance of GSH resynthesis upon exposure of tumor cells to SL was evaluated with the use of buthionine ...
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Hormone mediated down regulation of hepatic glutathione synthesis in the rat
... reduced glutathione (GSH) ...GSH synthesis in cultured hepatocytes by ...GSH synthesis from substrates (cysteine, ATP, glutamate, and glycine) by approximately 20% which was prevented by the A-kinase ...
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High-Dose Cysteine Administration Does Not Increase Synthesis of the Antioxidant Glutathione Preterm Infants
... of data, which makes it hard to draw firm conclusions regarding the upper limit of cysteine intake in preterm in- fants. We explicitly chose not to seek the upper limits of cysteine intake. We performed our measurements ...
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Systematic manipulation of glutathione metabolism in Escherichia coli for improved glutathione production
... SdaA, SdaB and TdcG [28–30]. SdaA was reported to be the gene encoding either the structural gene of L-SD that catalyzes the deamination of serine to pyruvate or a posi- tive activator of transcription [31]. To enhance ...
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Chronic ethanol ingestion impairs alveolar type II cell glutathione homeostasis and function and predisposes to endotoxin mediated acute edematous lung injury in rats
... First, glutathione is a critical antioxi- dant in the alveolar lining fluid that is synthesized in the liver and exported via the plasma to other tissues including the ...tissue glutathione levels are ...
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Current status and emerging role of glutathione in food grade lactic acid bacteria
... Glutathione synthesis, transport and metabolism in Lactobacillus genus have been studied for some species but most are yet to be ...de-novo synthesis could not be established ...of glutathione ...
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Hepatobiliary transport of glutathione and glutathione conjugate in rats with hereditary hyperbilirubinemia
... of glutathione and a glutathione conjugate was studied in normal Wistar rats and TR- ...that glutathione is virtually absent from the bile of TR- ...of glutathione and the glutathione ...
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Glutathione-Assisted Synthesis of Hierarchical PbS Via Hydrothermal Degradation and Its Application in the Pesticidal Biosensing
... Thiol biomolecule of glutathione (GSH) combined with lead ion as precursor for synthesis of hierarchical PbS via hydrothermal degradation has been developed. The precursor and as-prepared PbS were ...
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Regulation of Hepatic Glutathione Turnover in Rats In Vivo and Evidence for Kinetic Homogeneity of the Hepatic Glutathione Pool
... The synthesis of glutathione could, therefore, be estimated from the rate constant and the intrahepatic glutathione ...hepatic synthesis of glutathione increased from ...
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Effect of Atrazine on Glutathione Levels, Glutathione S-Transferase and Glutathione Reductase Activities in Pea and Wheat Plants
... Free thiol group contents in treated pea plants were higher than in the control plants, following a tendency similar to that of the total glutathione (Figure 1A and 1E). In contrast, free thiol group contents in ...
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Erythrocyte glutathione synthetase deficiency leads not only to glutathione but also to glutathione S transferase deficiency
... erythrocytes using a sensitive cycling assay. The deficiency was found to be most severe in reticulocyte-depleted preparations. The GSH-S activity of the erythrocytes of the parents was one-half normal, while the ...
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HUMAN GLUTATHIONE S-TRANSFERASE A1-1 BINDING WITH NATURALLY OCCURRING LIGANDS: ASSESSMENT BY DOCKING SIMULATIONS
... ABSTRACT: Glutathione S-transferases are phase-II enzymes associated with detoxification and resistance to drugs and ...class Glutathione S-Transferase ...using Glutathione S-transferases A1-1 ...
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Novel metabolic biomarkers related to sulfur-dependent detoxification pathways in autistic patients of Saudi Arabia
... The key biological activities of Trx that are applicable to human disease can be categorized as antioxidant, growth promoting, anti-apoptotic and inflammation modulating [48]. Beyond its protective role, the Txn sys- tem ...
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