Glycoside hydrolase
Glycoside hydrolase family 32 is present in Bacillus subtilis phages
... a glycoside hydrolase 32 (GH32) family protein, observed for the first time in a viral genome and shown to have been acquired by LGT from the bacterial host of a ...
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The genes encoding glycoside hydrolase family 6 and 7 cellulases from the brown-rot fungus Coniophora puteana
... encoding glycoside hydrolase (GH) family 6 and 7 cellulases (cel6A, cel6B, cel7A, and cel7B) were obtained from the brown-rot fungus Coniophora puteana by genomic polymerase chain reaction (PCR) using ...
5
Natural diversity of glycoside hydrolase family 48 exoglucanases: insights from structure
... the glycoside hydrolase family 48 are believed to be an important member of many bacterial cellulase systems which also lack the better studied GH6 and GH7 family cellobiohydrolases produced by most ...
9
Structure and function of a glycoside hydrolase family 8 endoxylanase from Teredinibacter turnerae
... Applying the CAZY classification for carbohydrate-active enzymes (http://www.cazy.org; see Lombard et al., 2014) to the T. turnerae genome reveals many potential xylanases, including ten GH10 enzymes, five GH11 enzymes ...
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Structure and function of a glycoside hydrolase family 8 endoxylanase from Teredinibacter turnerae
... Applying the CAZY classification for carbohydrate-active enzymes (http://www.cazy.org; see Lombard et al., 2014) to the T. turnerae genome reveals many potential xylanases, including ten GH10 enzymes, five GH11 enzymes ...
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Structural Enzymology of Cellvibrio japonicus Agd31B Protein Reveals α-Transglucosylase Activity in Glycoside Hydrolase Family 31
... including glycoside hydro- lases, glycoside phosphorylases, and ...from glycoside hydrolase family 13 (GH13) and GH77 play well established roles in ␣ -glucan side chain (de)branching, ...
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Structural snapshots for mechanism based inactivation of a glycoside hydrolase by cyclopropyl carbasugars
... Cyclopropyl carbocycles inhibitors of a glycoside hydrolase act by covalently modifying the nucleophilic carboxylate while still retaining the strained cyclo- propyl ring. A combined synthetic, kinetic, and ...
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Spiro-epoxyglycosides as activity-based probes for glycoside hydrolase family 99 endomannosidase/endomannanase
... Abstract: N-Glycans direct protein function, stability, folding and targeting, and influence immunogenicity. While most glycosidases that process N-glycans cleave a single sugar residue at a time, enzymes from ...
10
Glycoside hydrolase gene transcription by Alicyclobacillus acidocaldarius during growth on wheat arabinoxylan and monosaccharides: a proposed xylan hydrolysis mechanism
... other glycoside hydrolase genes (Aaci_2869 and Aaci_2887) behaved in the opposite manner of the two genes just discussed and were upregulated when xylose was added but downregulated with ...15 ...
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Characterization of β-N-acetylhexosaminidase (LeHex20A), a member of glycoside hydrolase family 20, from Lentinula edodes (shiitake mushroom)
... We purified and cloned a β -N-acetylhexosaminidase, LeHex20A, with a molecular mass of 79 kDa from the fruiting body of Lentinula edodes (shiitake mushroom). The gene lehex20a gene had 1,659 nucleotides, encoding 553 ...
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Contribution of a family 1 carbohydrate-binding module in thermostable glycoside hydrolase 10 xylanase from Talaromyces cellulolyticus toward synergistic enzymatic hydrolysis of lignocellulose
... Results: Thermostable endo- β -1,4-xylanase (Xyl10A) from Talaromyces cellulolyticus, which is recognized as one of the core enzymes in the fungal cellulase system, has a modular structure consisting of a ...
13
In vitro and in vivo characterization of three Cellvibrio japonicus glycoside hydrolase family 5 members reveals potent xyloglucan backbone-cleaving functions
... three glycoside hydrolase family 5 subfam- ily 4 (GH5_4) members, with distinct modular organization, as potential keystone endo-xyloglucanases in ...
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A multifunctional thermophilic glycoside hydrolase from Caldicellulosiruptor owensensis with potential applications in production of biofuels and biochemicals
... The gene Calow_0296 consists of a 1359 bp fragment encod- ing 452 amino acids, which belongs to glycoside hydrolase family 1 (GH1) and was named CoGH1A. The predicted molecular weight of CoGH1A was 53.2 ...
13
Comparative Community Proteomics Demonstrates the Unexpected Importance of Actinobacterial Glycoside Hydrolase Family 12 Protein for Crystalline Cellulose Hydrolysis
... and glycoside hydrolase ac- ...Cellobio- hydrolase (pNPC), - D -glucosidase (pNPG), and - D -xylosidase (pNPX) activities were determined using their respective p-nitrophenyl sugar sub- ...
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Unravelling the diversity of glycoside hydrolase family 13 α-amylases from Lactobacillus plantarum WCFS1
... p-nitrophenyl glycoside derivatives: pNP- α-d-galactopyranoside, pNP-α-d-glucopyranoside, pNP- α-d-manopyranoside, pNP-α-d-xylopyranoside, pNP-α- L-arabinofuranoside, pNP-α-L-arabinopyranoside, pNP- ...
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Determination of glycoside hydrolase specificities during hydrolysis of plant cell walls using glycome profiling
... To better understand the specificity of enzyme hydrolysis within the complex matrix of polysaccharides found in the plant cell wall, we studied the reactions of individual enzymes usin[r] ...
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The GH10 and GH48 dual-functional catalytic domains from a multimodular glycoside hydrolase synergize in hydrolyzing both cellulose and xylan
... Taken together, our results demonstrated for the first time that the GH10 and GH48 catalytic domains, which are often simply recognized as xylanase and cellulase, respectively, can cooperate in hydrolyzing both cellulose ...
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Engineering the glycoside hydrolase β-glucuronidase (β-GUS) to improve a non-native activity for the synthesis of O-glucuronides
... Scheme I: Two-step indirect approach Scheme II: One-step direct approach Evolve -GUS for higher activity at higher substrate concentration Site specific mutagenesis of -GUS 504 E G to cr[r] ...
228
In vitro and in vivo characterization of three Cellvibrio japonicus Glycoside Hydrolase Family 5 members reveals potent xyloglucan backbone-cleaving functions
... To determine specific activity values of CjGH5 enzymes toward XyG, final concentration of 0.75, 2.59, and 0.71 nM of the recombinant purified catalytic modules CjGH5D, CjGH5E, and CjGH5F[r] ...
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Comparative insights into the saccharification potentials of a relatively unexplored but robust Penicillium funiculosum glycoside hydrolase 7 cellobiohydrolase
... Furthermore, we built a PfCBH1 three-dimensional structure using the TrCBH1 structure as a template and conducted molecular dynamics simulations to compare the structural differences [r] ...
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