Glycosylation sites
Identification of Two N-Linked Glycosylation Sites within the Core of the Simian Immunodeficiency Virus Glycoprotein Whose Removal Enhances Sensitivity to Soluble CD4
... nine glycosylation mutants of SIV239 in which the eliminated N-linked sites were predicted to lie greater than 10 Å from CD4 contact residues (Table ...at glycosylation sites 14 and 15 ...
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Loss of Asparagine-Linked Glycosylation Sites in Variable Region 5 of Human Immunodeficiency Virus Type 1 Envelope Is Associated with Resistance to CD4 Antibody Ibalizumab
... Ibalizumab (formerly TNX-355) is a first-in-class, monoclonal antibody inhibitor of CD4-mediated human immunodeficiency type 1 (HIV-1) entry. Multiple clinical trials with HIV-infected patients have demonstrated the ...
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Mutation of Putative N-Glycosylation Sites on Dengue Virus NS4B Decreases RNA Replication
... NS4B glycosylation in various stages of the virus life ...N-glycosylation sites affected the replication step in both mammalian and mosquito C6/36 cell culture ...
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Redesigning N-glycosylation sites in a GH3 β-xylosidase improves the enzymatic efficiency
... Several post-translational modifications (PTMs) occur on microbial proteins, including those in bacteria and fungi. N-glycosylation is one of the most important PTMs—influencing protein secretion, stability, ...
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Role of conserved glycosylation sites in maturation and transport of influenza A virus hemagglutinin.
... Expression of the altered HA proteins in eukaryotic cells by a panel of recombinant vaccinia viruses revealed that rates and efficiency of intracellular transport of HA are dependent upo[r] ...
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Glycosylation sites of vesicular stomatitis virus glycoprotein.
... The virus pellet was suspended in 1 ml of ET buffer by sonic treatment and centrifuged through a 5 to 20% ET-sucrose gradient in an SW27 rotor at 871 Downloaded from http://jvi.asm.org/ [r] ...
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Study of carbon distribution at glycosylation sites in Arabidopsis thaliana
... The post-translational addition of carbohydrate to proteins plays a significant role in overall biochemical complexity in humans (as well as all eukaryotes). The magnitude of this protein modification can be emphasized ...
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Role of N-Linked Glycans in the Functions of Hepatitis C Virus Envelope Proteins Incorporated into Infectious Virions
... for glycosylation studies, since HCV tropism is restricted to the ...protein glycosylation have been observed between HCVpp and HCVcc particles ...N-linked glycosylation sites in the context ...
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Canine Distemper Viruses Expressing a Hemagglutinin without N-Glycans Lose Virulence but Retain Immunosuppression
... N glycosylation to morbillivirus attachment (H) protein function and overall virulence, we first determined the use of the potential N-glycosylation sites in the canine distemper virus (CDV) H ...
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Mutational Analysis of Glycosylation, Membrane Translocation, and Cell Surface Expression of the Hepatitis E Virus ORF2 Protein
... pORF2 glycosylation? It is not clear if the addition of N-glycans has any role to play in viral capsid assembly or virus-host cell ...N-linked glycosylation sites and is translocated to the ER, where ...
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Model for intracellular folding of the human immunodeficiency virus type 1 gp120.
... Interestingly, incomplete endoglycosidase H treatment revealed that a partially glycosylated glycoprotein could bind to the receptor, implying that a subset of glycosylation sites, perha[r] ...
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Sequences in Glycoprotein gp41, the CD4 Binding Site, and the V2 Domain Regulate Sensitivity and Resistance of HIV-1 to Broadly Neutralizing Antibodies
... Previous studies have reported polymorphisms in the C1 do- main (25), the V2 domain (18, 40, 42, 48, 50, 57, 63), the ␣ 2 amphipathic helix in the C3 domain (50), the CD4 binding site (11, 12), and the C34 helix of gp41 ...
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Comparison of Viral Env Proteins from Acute and Chronic Infections with Subtype C Human Immunodeficiency Virus Type 1 Identifies Differences in Glycosylation and CCR5 Utilization and Suggests a New Strategy for Immunogen Design
... of glycosylation sites had differential effects on neutralization sensitivity for different ...of glycosylation sites encoded in the conserved regions of env, further reduced in transmitted ...
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Characterization of human immunodeficiency virus type 1 gp120 binding to liposomes containing galactosylceramide.
... Assignment of intrachain disulfide bonds and characterization of potential glycosylation sites of the type I recombinant human immunodeficiency virus envelope glycoprotein gp120 expresse[r] ...
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Essential Role of Dengue Virus Envelope Protein N Glycosylation at Asparagine-67 during Viral Propagation
... DV glycosylation mutants propagated in mosquito cells ...E glycosylation for DV replication in mammalian and mosquito cells can be due to differing E se- cretion in the two host cells ...the ...
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Logic minimization and rule extraction for identification of functional sites in molecular sequences
... The proposed logic minimization method provides sets of rules for predicting TFBS on human DNA and O-glycosylation sites on proteins with sensitivity and PPV comparable to those from ANN and SVM. ...
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Modulation of ecotropic murine retroviruses by N-linked glycosylation of the cell surface receptor/amino acid transporter.
... mCAT-1 glycosylation were previously analyzed by Kim and Cunningham (17) in a study that used antiserum to mCAT-1 as well as site-directed ...potential glycosylation sites in loop 3 are glycosylated ...
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Structure/function relationships of the esterase 6 protein of Drosophila melanogaster
... The sequencing of clones containing the esterase 6 structural gene shows that it encodes a protein of the correct molecular weight, containing four potential N-linked glycosylation sites[r] ...
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Mullan_Redacted.pdf
... Chikungunya virus is an arthropod-borne alphavirus that, in recent years, has presented an increased threat to human populations as the Aedes aegypti mosquitos which carry it have experienced resurgence in their ...
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Glycosylation of gp41 of Simian Immunodeficiency Virus Shields Epitopes That Can Be Targets for Neutralizing Antibodies
... attachment sites within gp120 without a loss of the ability to bind CD4 or the ability to yield replication-competent ...of glycosylation sites, particularly within the V1/V2 and V3 loops but also on ...
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