GPCRs/G protein
Mini G protein probes for active G protein– coupled receptors (GPCRs) in live cells
... G protein– coupled receptors (GPCRs) are key signaling pro- teins that regulate nearly every aspect of cell ...of GPCRs have benefited greatly from the development of molec- ular tools to ...
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Chemical-Scale Studies of G Protein-Coupled Receptors and Ligand-Gated Ion Channels
... FP-tagged G proteins and GPCRs by FRET, principally using the FRET pair of Luciferase (a protein yielding bioluminescence when supplied with the substrate coelenterazine) as the donor and GFP as the ...
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Identification of G protein coupled receptors in Schistosoma haematobium and S mansoni by comparative genomics
... schistosomes. GPCRs have been shown to be valuable drug target candidates in some organisms, but key functional mechanisms of mem- bers of this complex superfamily still require detailed in- vestigations ...of ...
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Polycystin 1 loss of function is directly linked to an imbalance in G protein signaling in the kidney
... a G-protein-coupled receptor ...a G-protein binding domain (GBD), which binds to multiple G α subunits (Parnell et ...adhesion GPCRs, a subgroup of at least 33 GPCRs in ...
11
Proteinase-activated receptors (PARs) – focus on receptor-receptor-interactions and their physiological and pathophysiological impact
... classical G protein-mediated pathways and beta-arrestin ...other GPCRs, toll-like receptors and NOD-like receptors, ion channel receptors, and on PAR association with cargo ...
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A semantics-oriented computational approach to investigate microRNA regulation on glucocorticoid resistance in pediatric acute lymphoblastic leukemia
... in G protein-coupled receptors (GPCRs) ...activated GPCRs at the plasma membrane, β-arrestin terminates G protein-dependent responses (desensitization) and stimulates ...
9
Mini G proteins : novel tools for studying GPCRs in their active conformation
... brane protein purification ...receptor–mini-G protein complexes. However, GPCRs in the active state are also unstable in detergent and this also needs to be carefully addressed before ...
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GPCR oligomers in pharmacology and signaling
... G protein-coupled receptors (GPCRs) [1], also known as seven transmembrane receptors, were initially consid- ered to function as monomeric structural ...of GPCRs are focused on the residues ...
7
Oligomerization of G-Protein-Coupled Receptors: Lessons from the Yeast Saccharomyces cerevisiae
... classical G protein heterotrimers? Heptaheli- cal GPCRs can signal independently of classical G protein heterotrimers in order to down-regulate receptors or transduce mitogenic or other ...
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The role of the G protein and cAMP/PKA signalling pathway in establishment and maintenance of the mutualistic Epichloë festucae ryegrass association : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Genetics at Massey University, Palmerston North, New Zealand
... on G protein-coupled receptors (GPCRs), which transmit signals predominantly via heterotrimeric G proteins to downstream pathways such as the cAMP/Protein Kinase A (PKA) and MAP kinase ...
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Active state structures of G protein coupled receptors highlight the similarities and differences in the G protein and arrestin coupling interfaces
... state GPCRs, the frequency of publication of these structures has been relatively low, nonetheless, during the past year three new structures have been reported that have significantly enhanced our knowledge of ...
20
G PROTEIN COUPLED RECEPTORS A POTENTIAL NEW DRUG TARGET TO COMBAT DIABETIC SYNDROME: AN OVERVIEW
... G protein-coupled receptors (GPCRs), also known as seven-transmembrane domain receptors, 7TM receptors, heptahelical receptors, serpentine receptor, and G protein-linked receptors ...
11
Engineering a minimal G protein to facilitate crystallisation of G protein coupled receptors in their active conformation
... Mini G proteins are novel tools that have many potential appli- cations, including characterisation of receptor pharmacology in response to different classes of agonists (full, partial and weak), binding affinity ...
12
Illuminating the life of GPCRs
... of GPCRs and will enable high-throughput screening appli- ...staphylococcal protein A ...for GPCRs asso- ciated tumor diagnosis and therapy ...
22
GPCRs through the keyhole: the role of protein flexibility in ligand binding to β adrenoceptors
... has the necessary plasticity to accommodate ligands bearing longer head groups than are present in any of the structures crystallised to date, without the need to propose a radically different binding mode. Remarkably, ...
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HINT1 protein cooperates with cannabinoid 1 receptor to negatively regulate glutamate NMDA receptor activity
... certain GPCRs, recruiting a series of signaling proteins to the receptor environment ...HINT1 protein associates with the cyto- solic regions of CNR1 [5,18,31], we sought to determine the effects of HINT1 ...
16
A formylpeptide receptor, FPRL1, acts as an efficient coreceptor for primary isolates of human immunodeficiency virus
... Cell line-adapted R5-X4 HIV-1 strains (GUN-1WT [57], GUN-4WT [59], and GUN-7WT [59]), GPR1-X4 HIV-1 variants (GUN-1V [57], GUN-4V [59], and GUN-7V [59]), an X4 HIV-1 strain (IIIB [60]), R5 HIV-1 strains (SF162 [61] and ...
14
Multiple Growth Factors, But Not VEGF, Stimulate Glycosaminoglycan Hyperelongation in Retinal Choroidal Endothelial Cells
... There is an increasing recognition that a number of major diseases are associated with the disposition of lipids in tissues [7-10]. Apolipoproteins on LDL particles become trapped and deposited into tissues where ...
11
Homology modeling a fast tool for drug discovery: Current perspectives
... a class of probabilistic models that are generally applicable to time series or linear sequence. Profile HMM are very effective in detecting conserved patterns in multiple sequences. The SATCHMO algorithm in the LOBSTER ...
17
Application of BRET to monitor ligand binding to GPCRs
... monitor protein-protein or drug-protein interactions with ease and sensitivity is the cornerstone of cell biology and ...fluorescent protein variant Venus are a notable example of a BRET ...
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