GPx1/2
MicroRNA-153/Nrf-2/GPx1 pathway regulates radiosensitivity and stemness of glioma stem cells via reactive oxygen species
... Some studies have reported that suppression of the Nrf-2-mediated antioxidant defense system sensitizes cancer cells to ionizing radiation and chemotherapeutic drugs [36, 38, 43]. We employed lentiviral-mediated ...
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Antioxidant capacity and concentration of redox-active trace mineral in fully weaned intra-uterine growth retardation piglets
... thioredoxin 2 (TXN2) and β-actin) are given in Table ...using 2 μL of cDNA (50 μg/mL), ...the 2 -ΔΔC T method [19], in which the β-actin gene ...
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A possible role for selenoprotein glutathione peroxidase (GPx1) and thioredoxin reductases (TrxR1) in thyroid cancer: our experience in thyroid surgery
... of GPx1 and TrxR1 expression in thyroid tissue The overall protein composition of each patient’s thy- roid tissue was determined by SDS-PAGE, and the pro- tein content was verified by a specific protein staining ...
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Original Article Association between glutathione peroxidase-1 (GPX1) Rs1050450 polymorphisms and cancer risk
... [37]. GPX1 plays a crucial role in the detoxification of mitochondrial ...of GPX1 could increase the anti- oxidant capacity in one cell, thus reducing intracellular oxi- dative ...of GPX1 levels has ...
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2D-DIGE proteome analysis on the platelet proteins of patients with major depression
... unmatched masses, the matched peptide sequences infor- mation, enzyme cleavage specificity, any modification and deviations were provided in the Additional file 1: Table S1. Eight proteins were identified among the ...
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Original Article Relationship between GPX1 rs1050450 variation and the onset risk of coronary artery disease
... table we could see that there existed CC, CT and TT three genotypes in cases and controls. The distribution frequencies of TT genotype were low in two groups, so we combined two mutant genotypes of CT and TT to detect ...
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Oxidative stress and inflammation in retained placenta: a pilot study of protein and gene expression of GPX1 and NFκB
... lar GPX1(1) protein in placental samples using the GPX1 human intracellular ELISA kit (Adipogen International ...polyclonal GPX1(1) antibody-specific coated well of the ELISA ...
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Extent of differential allelic expression of candidate breast cancer genes is similar in blood and breast
... Next, we analysed 40 normal breast tissue samples (green data points in Figure 1a). Like blood, breast is a complex organ, comprising breast epithelium, stroma, and adipocytes. The comparison between fresh blood and ...
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Oxido reductive regulation of vascular remodeling by receptor tyrosine kinase ROS1
... s-Glutathiolation of critical SHP-2 cysteines inhibits ROS1 deac- tivation. We next sought to determine the mechanism of ROS1 as a redox sensor in VSMCs. Disulfide bonds in proteins are formed by oxidation of the ...
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Relationship between oxidizable fatty acid content and level of antioxidant glutathione peroxidases in marine fish
... Comparisons of tissue-specific PUFA content and GPx4 activity, as well as in vitro feeding studies in mammals indicate a link between unsaturation level of tissue lipids and the activity of GPx isozymes in other ...
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Serum selenium and single-nucleotide polymorphisms in genes for selenoproteins: relationship to markers of oxidative stress in men from Auckland, New Zealand
... The significant association of GPx activity with serum Se level seen among GPx1 rs1050450 CC genotype indi- viduals is similar to observations made by Xiong et al. (2010). There are other significant correlations ...
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The hsa miR 181a 5p reduces oxidation resistance by controlling SECISBP2 in osteoarthritis
... To assess the role of SBP2 in chondrocytes, we con- structed recombinant hSBP2-CDS clones and si-SBP2 (Fig. 2a) and transfected these constructs into SW1353 cells. Exogenous SBP2 (122 kDa, Fig. 2b) showed re- markable ...
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Supplementation of a grape seed and grape marc meal extract decreases activities of the oxidative stress-responsive transcription factors NF-κB and Nrf2 in the duodenal mucosa of pigs
... ligand 2; CYP1A1: Cytochrome P450, family 1, subfamily A, polypeptide 1; GPX1: Glutathione peroxidase 1; GSGME: Grape seed and grape marc meal extract; HMOX1: Heme oxygenase 1; HP: Haptoglobin; HPTLC: High- ...
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Original Article Oxidative stress contributes to abnormal glucose metabolism and insulin sensitivity in two hyperlipidemia models
... CAT, Gpx1; ...CAT, Gpx1; E. In visceral fat, relative mRNA expressions of SOD1, CAT, Gpx1; ...CAT, Gpx1; G, ...Catalase; Gpx1, glutathione peroxidase 1; Nrf2, nuclear factor erythroid ...
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<p>Anti-Oxidant and Anti-Endothelial Dysfunctional Properties of Nano-Selenium in vitro and in vivo of Hyperhomocysteinemic Rats</p>
... 1, 2, 4, 6 mg Se/kg of Nano-Se for 10 consecutive ...of GPX1 activity and a part was fi xed in cold 4% formaldehyde for histopathological assessment of liver ...
21
MMP expression alteration and MMP-1 production control by syringic acid via AP-1 mechanism
... acid, 2 ′ -7 ′ -dichlorofluorescein diacetate was used to measure the ROS ...mRNA, GPx1 mRNA, and catalase ( CAT ) mRNA, which are related to oxidation ...
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Original Article Pathways and protective mechanisms for cerebral ischemia-reperfusion injuries in mice
... Results of GO analysis for the 1,711 DEGs in WT mice cortexes within 24 hours postreperfu- sion indicated that they were mainly associated with intracellular signaling cascades, lympho- cyte activation during immune ...
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Erythrocyte selenium concentration predicts intensive care unit and hospital mortality in patients with septic shock: a prospective observational study
... evaluated GPx1 activity and its Pro198Leu ...exon 2 of the gene, promoting an amino acid change from proline to leucine at codon 198 ...study, GPx1 activity was higher in patients carrying the ...
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MnSOD and GPx1 polymorphism relationship with coronary heart disease risk and severity
... The second line of enzymatic antioxidant defense is played by glutathione peroxidase (GPx) isoenzymes. GPx has 6 isoforms (GPx-1-6) [15]. GPx-1 is the most widely distributed and abundant form in human cells, includ- ing ...
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Genetic polymorphism in selenoprotein P modifies the response to selenium-rich foods on blood levels of selenium and selenoprotein P in a randomized dietary intervention study in Danes
... In Table 3, the associations between mean erythrocyte GPX enzyme activity, concentrations of whole blood selen- ium and plasma selenoprotein P, and the studied polymor- phisms, and within-subject effects between genotype ...
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