GRADIENT GEL-ELECTROPHORESIS
Search for Localized Dysbiosis in Crohn's Disease Ulcerations by Temporal Temperature Gradient Gel Electrophoresis of 16S rRNA
... temperature gradient gel electrophoresis (TTGE) to compare the qualitative com- positions of the mucosa-associated microbiotas in ulcerated (U) and nonulcerated (NU) regions of the ilea and colons of ...
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Temperature Gradient Gel Electrophoresis of Fecal 16S rRNA Reveals Active Escherichia coli in the Microbiota of Patients with Ulcerative Colitis
... Previous studies of the endogenous microbiota in patients with ulcerative colitis (UC) have not taken bacterial activity into account, yet bacteria with high transcriptional activity might have a more important ...
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Statistical Analyses of Complex Denaturing Gradient Gel Electrophoresis Profiles
... Studies using molecular techniques have demonstrated that a culture-based approach can severely under- estimate the bacterial diversity in most environments. One of the molecular techniques that has been applied in ...
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Denaturing Gradient Gel Electrophoresis as a Diagnostic Tool in Periodontal Microbiology
... denaturing gradient gel electrophoresis (DGGE) for the study of total oral microbial populations has been shown previously (4, 23, ...polyacrylamide gel containing a linear gradient of ...
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Temperature Gradient Gel Electrophoresis as a Valuable Accessory Tool for Assessment of Dysbiosis in Crohn’s Disease
... or electrophoresis in denaturing gels of 16SrDNA ...temperature gradient gel electrophoresis (TGGE) and next generation sequencing of 16SV6-V8rDNA amplicons present in gram negative cultures ...
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Denaturing Gradient Gel Electrophoresis of PCR Amplified gki Genes: a New Technique for Tracking Streptococci
... Viridans group streptococci (VGS) are a well-known cause of infections in immunocompromised patients, accounting for severe morbidity and mortality. Streptococcus mitis group species (Streptococcus mitis, Strepto- coccus ...
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High Prevalence of Helicobacter Species Detected in Laboratory Mouse Strains by Multiplex PCR Denaturing Gradient Gel Electrophoresis and Pyrosequencing
... Rodent models have been developed to study the pathogenesis of diseases caused by Helicobacter pylori, as well as by other gastric and intestinal Helicobacter spp., but some murine enteric Helicobacter spp. cause ...
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Sequence Polymorphisms in a Surface PPE Protein Distinguish Types I, II, and III of Mycobacterium avium subsp paratuberculosis
... denaturing gradient gel electrophoresis, allowing visual discrimination of the three subtypes of ...denaturing gradient gel electrophoresis on a single gene as a method to ...
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Metagenomic rDNA Profiles of the Oral Microbiome in Healthy Subjects Are Distinctive Compared to That in Smokers and Disease Subjects Despite High Variations between Subjects
... tured gradient gel electrophoresis (PCR-DGGE) of the 16S rDNA gene in me- tagenomic samples, this study evaluated the stability of the oral microbiome and contrasted the PCR-DGGE profiles of subjects ...
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Denaturing gradient gel electrophoresis analysis of archaeal and bacterial populations in a submerged anaerobic membrane bioreactor treating landfill leachate at low temperatures
... The archaeal populations from the HR, SAMBR30 and SAMBR300 were analyzed using the DGGE technique as described in Material and Methods, and the gel picture is shown in Fig. 3. The brightest bands were cut and ...
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Structural Shifts of Mucosa Associated Lactobacilli and Clostridium leptum Subgroup in Patients with Ulcerative Colitis
... Denaturing gradient gel electrophoresis analysis was employed to profile the composition of the dominant bacteria (16S rRNA gene V3 region) and three important groups: lactobacilli, the Clostridium ...
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Denaturing gradient gel electrophoresis as a fingerprinting method for the analysis of soil microbial communities
... by electrophoresis in a gradient of a ...temperature gradient is used as the denaturant in addition to the chemical compounds in the ...denaturing gradient, DNA fragments migrate under the ...
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Identification of the multiple beta thalassemia mutations by denaturing gradient gel electrophoresis
... denaturing gradient gel electrophoresis to detect the beta-thalassemia mutations in the Chinese ...denaturing gradient gel electrophoresis offers a nonradioactive means of ...
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Rapid Discrimination of Listeria monocytogenes Strains by Microtemperature Gradient Gel Electrophoresis
... Microtemperature gradient gel electrophoresis ( -TGGE) was examined for use for the rapid subtyping of Listeria monocytogenes ...total electrophoresis time of only 7 ...
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Phytase producing Bacillus sp inoculation increases phosphorus availability in cattle manure
... improve Po hydrolysis in manure with a concomitant increase in P availability for plants requires further evaluation. The primary concerns regarding the use of these bacteria as manure inoculants are the inoculum ...
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Effect of legume cereal mixtures on the diversity of bacterial communities in the rhizosphere
... Aboveground plant diversity is known to influence belowground diversity and ecosystem processes. However, there is little knowledge of soil microbial succession in legume-grass mixtures. Therefore, this study was ...
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PREPARATIVE ELECTROPHORESIS pdf
... preparative gel elec- trophoresis systems in the early 1960s, a range of different instrument designs have also been developed to incorporate ...owing electrophoresis, the use of an- ticonvective media in ...
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CLINICAL APPLICATIONS Gel Electrophoresis pdf
... able. Electrophoresis on cellulose acetate is employed to study many different enzymes such as serum amylase, pancreatic isolipase, and pyruvate ...Agarose gel electrophoresis is extensively used to ...
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Platelet glycoproteins Ia, Ic, and IIa are physicochemically indistinguishable from the very late activation antigens adhesion related proteins of lymphocytes and other cell types
... two-dimensional gel electrophoresis, we show that reduced VLA molecules of platelets are composed of three chains of molecular weights 165,000, 145,000, and 140,000 that possess the physicochemical ...
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Synovial membrane protein expression differs between juvenile idiopathic arthritis subtypes in early disease
... Introduction: Juvenile idiopathic arthritis (JIA) is the most common rheumatological disease of childhood with a prevalence of around 1 in 1,000. Without appropriate treatment it can have devastating consequences ...
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