Granulosa Cells
The Cultivation of Human Granulosa Cells
... of granulosa cells (GCs) include the production of steroids, as well as a myriad of growth factors to interact with the oocyte during its development within the ovarian ...thecal cells) to estradiol ...
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Wt1 directs the lineage specification of sertoli and granulosa cells by repressing Sf1 expression
... steroidogenic cells in male and female gonads is most likely regulated by a common ...somatic cells before sex ...and granulosa cells was blocked and most somatic cells in both male and ...
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Assessment of PGC1α-FNDC5 Axis in Granulosa Cells of PCOS Mouse Model
... and atretic follicles which contained degenerated oocytes in DHEA-treated mice. DHEA treatment also yielded formation of follicular cysts which was characterized by a compacted formation of granulosa cells ...
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Selective expression of KrasG12D in granulosa cells of the mouse ovary causes defects in follicle development and ovulation
... wild-type granulosa cells by eCG/hCG ...in granulosa/cumulus cells at 4 hours after hCG (D), but not in immature wild-type ovaries ...unstimulated granulosa cells (I) or those ...
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Heat shock pretreatment of mesenchymal stem cells for inhibiting the apoptosis of ovarian granulosa cells enhanced the repair effect on chemotherapy-induced premature ovarian failure
... Methods: Rat MSCs were isolated, cultured and identified. At 24 h, 48 h and 72 h after different strengths of HS pretreatment for 30 min, 1 h, 2 h and 3 h, apoptosis of MSCs was detected to determine the optimal ...
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Influence of mycotoxin zearalenone and its derivatives (alpha and beta zearalenol) on apoptosis and proliferation of cultured granulosa cells from equine ovaries
... Follicular degeneration, or atresia, is the result of an apop- totic process defined as a sequence of programmed intra- cellular events leading to cell death [20]. The first signs of atresia are evidenced as the ...
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Expression of pluripotency markers in human granulosa cells after embryonic stem cell extract exposure and epigenetic modification
... markers expression. Various reports showed that the pluripotency markers are expressed at different period of times (6, 10, 11). Some pluripotency markers such as Sox2 were detected only after 8 weeks of culturing (10). ...
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Oxygen consumption of bovine granulosa cells in vitro : a thesis presented in partial fulfilment of the requirements for the degree of Master of Engineering in Biotechnology at Massey University, Palmerston North, New Zealand
... transport. Granulosa cell viability tests show that this chilled condition does not affect the viability of the granulosa cells (Yada et ...ovarian cells; thus, the build-up of the products of ...
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4-Aminopyridine Decreases Progesterone Production by Porcine Granulosa Cells
... pig granulosa cells (Mol Phar- macol 2002 ...PGC-2 cells express K + currents similar to the granulosa cell ultra-rapid delayed rectifier cur- rent (I Kur ) in terms of the voltage-dependence ...
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Proteomic characterization of bovine granulosa cells in dominant and subordinate follicles
... GCs: Granulosa cells; GO: Gene Ontology; GPCRs: G protein-coupled receptors; HSP: Heat shock protein; KEGG: Kyoto Encyclopedia of Genes and Genomes; LC-MS/MS: Capillary High Performance Liquid ...
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Markers of stem cells in human ovarian granulosa cells: is there a clinical significance in ART?
... on granulosa cells in order to overcome possible limita- ...on granulosa cells are going to reinforce our ...luteinized granulosa cells is a new field of knowledge and according ...
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Dopamine receptor repertoire of human granulosa cells
... on granulosa cells (GCs) of the follicle ...luteal cells are reported to perform synthesis of NE in a similar way [6] and presumably this occurs also in intrao- varian nerve fibers and neuron-like ...
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Interleukin-1 (IL-1) system gene expression in granulosa cells: kinetics during terminal preovulatory follicle maturation in the mare
... a granulosa cell IL-1 system consisting of ligands (IL-1β rather than IL-1α), receptors (IL-1R1 and/or IL- 1R2), and receptor ...equine granulosa cells suggests its in- volvement in equine ovarian ...
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Identification and characterization of long non-coding RNAs in porcine granulosa cells exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin
... DELs were indirectly predicted via searching their target cis - and trans -regulated protein-coding genes. The co- expression analysis revealed that DELs may influence the expression of numerous genes, including those ...
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Follicular stage-dependent regulation of apoptosis and steroidogenesis by prohibitin in rat granulosa cells
... of granulosa cell isolation could account for the different outcome, remains to be de- ...in granulosa cells ...human granulosa cells while the opposite was true with higher dosages ...
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Modulation of gonadotrophin induced steroidogenic enzymes in granulosa cells by d-chiroinositol
... 35. Yong EL, Hillier SG, Turner M, Baird DT, Ng SC, Bongso A, Ratnam SS. Differential regulation of cholesterol side-chain cleavage (P450scc) and aromatase (P450arom) enzyme mRNA expression by gonadotropins and cyclic ...
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Docosahexaenoic acid (DHA) effects on proliferation and steroidogenesis of bovine granulosa cells
... viable cells/250 μL media/well) in modified McCoy’s 5A medium in the presence or absence of DHA (1, 10 or 50 μM) for 15 ...removed. Cells with the same treatment (pool of 12 wells) were isolated in PBS 1X ...
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Laparoscopic restaging of ovarian granulosa cells tumor: three cases report
... In our three cases, laparoscopy was safe and effective. No spillage occurred during the first diagnostic approach, and a complete staging was possible during the restaging procedure. An advantage of laparoscopy is that ...
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BRCA1, Hormone, and Tissue-Specific Tumor Suppression
... ovarian granulosa cells containing inherited mu- tant BRCA1 would have elevated basal aromatase expression ...human granulosa cell line that maintains physiological response to cAMP-mediated ...
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THE EXPRESSION OF APELIN AND ITS RECEPTOR APJ DURING DIFFERENT PHYSIOLOGICAL STAGES IN THE BOVINE OVARY
... Entire reproductive tracts from cows were col- lected at a local slaughterhouse within 10–20 min after slaughter and were transported on ice to the labora- tory. The stage of the oestrous cycle was defined by macroscopic ...
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