Granzyme B
Protease-activated receptor-1 activation by granzyme B causes neurotoxicity that is augmented by interleukin-1β
... as granzyme B, IL-1β, or inhibitors. To in- hibit granzyme B activity in CSF samples, these samples were pretreated with the selective cell- permeable protease inhibitor Z-Ala-Ala-Asp-chloro- ...
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Activating KIR Copy Number Variation Is Associated with Granzyme B Release by NK Cells during Primary Simian Immunodeficiency Virus Infection in Rhesus Monkeys
... Lower granzyme B content in the cytolytic NK cell subsets from monkeys expressing higher numbers of KIR3DH copies was ob- served during primary SIV ...intracellular granzyme B levels increased ...
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Perforin, granzyme B, and FasL expression by peripheral blood T lymphocytes in emphysema
... (PBS) supplemented with 1% bovine serum albumine (BSA) (Sigma-Aldrich Canada Ltd; Oakville, ON, Canada) were incubated with appropriate antibodies or isotype controls for 40 min on ice and protected from light. For ...
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Original Article Co-expression of perforin and granzyme B genes induces apoptosis and inhibits the tumorigenicity of laryngeal cancer cell line Hep-2
... 26]. Granzyme B-induced apoptosis is dependent on the presence of perforin because granzyme B cannot initiate binding to lipid cell mem- branes, and granzyme B internalization ...
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Follicular regulatory T cells inhibit the development of granzyme B–expressing follicular helper T cells
... GC B cells, we used RNA sequencing (RNAseq) to profile gene expression in TFH cells from PCT-challenged WT, Bcl6FC, Blimp1FC, and DKO mice (Figure 2 and Supplemental Figure 2A; sup- plemental material available ...
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HIV-Specific Granzyme B-Secreting but Not Gamma Interferon-Secreting T Cells Are Associated with Reduced Viral Reservoirs in Early HIV Infection
... and granzyme B responses suggests that one effector cytokine may be more important for initial virus control ...(i.e., granzyme B), whereas other cytokines may be more important for the ...
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Immunotherapeutic targeting of membrane Hsp70-expressing tumors using recombinant human granzyme B.
... [29–32]. In comparison to monolayer cells, a 20-fold higher concentration of human grB was required to induce the shrinkage of tumor spheroids. In contrast, significant active caspase-3 positivity could be achieved in ...
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Development of a human immuno-oncology therapeutic agent targeting HER2: targeted delivery of granzyme B
... and B cell mediated killing of tumor cells has met with clinical success in recent years suggesting tremendous potential for treatment in a broad spectrum of tumor ...and B cells, delivery of the serine ...
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Effector cell mediated cytotoxicity measured by intracellular Granzyme B release in HIV infected subjects
... (8). Granzyme B is known to be present in the cytotoxic granules of NK cells and activated CTLs with cytotoxic ...of Granzyme B release by effector cells and simultaneous analysis of effector ...
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Original Article Impact of different blood transfusion methods on the expression of perforin and granzyme B in patients with tumors
... ④ , followed by incubation for 30 min at 4°C in the dark. The cells were subjected to fluores- cent antibody staining of PFP and Gzm (Shang- hai Yuayi-Biotech Co., Ltd., Shanghai, China). After washing with ...
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A novel Granzyme B nanoparticle delivery system simulates immune cell functions for suppression of solid tumors
... the granzyme-based ...A, B, H, K, and M, are found in humans, among which granzyme B is the most abundant type with high cytotoxic efficacy and a variety of apoptosis-inducing mechanisms ...
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The role of granzyme B and other granule proteases as modulators of cytokine activity
... 5.2 Results 5.2.1 IL-1a activity assay 5.2.2 Granzyme B-mediated proteolysis ofIL-1a enhances its bioactivity in vitro 5.2.3 IL-1a is cleaved by other granule proteases 5.2.4 Cleavage of[r] ...
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<p>Dinutuximab Synergistically Enhances the Cytotoxicity of Natural Killer Cells to Retinoblastoma Through the Perforin-Granzyme B Pathway</p>
... and B cells (Figure 3A and B); however, granzyme B-positive NK cells were not detected in all tissue sections, probably due to the low percentage of NK cells or the small propor- tion of ...
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A Microfluidic Platform for Single Cell Fluorometric Granzyme B Profiling
... cell Granzyme B (GrB) activity was detected and measured fluorometrically using a commercial assay kit with a peptide substrate containing GrB recognition sequence (Ac-IEPD-AFC) and AFC ...
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Granzyme B-inhibitor serpina3n induces neuroprotection in vitro and in vivo
... We have previously shown that activated T cells induce hu- man neuronal death in vitro [11, 14] via the release of the serine protease granzyme B [14]. Both CD4 + and CD8 + T cells express GrB and induce ...
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Local distribution analysis of cytotoxic molecules in liver allograft is helpful for the diagnosis of acute cellular rejection after orthotopic liver transplantation
... Perforin, granzyme B and TIA-1 were mainly located in the cytoplasm of inflammatory cells in portal tracts and lobules, and they were frequently observed in the epithe- lia of interlobular bile ducts and ...
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Comprehensive analysis of the percentage of surface receptors and cytotoxic granules positive natural killer cells in patients with pancreatic cancer, gastric cancer, and colorectal cancer
... and granzyme B are intracellular molecules present in a number of lymphocytes, including NK ...of granzyme B to pro- mote apoptosis in target cells ...and granzyme B, and the ...
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Natural killer cells control a T helper 1 response in patients with Behçet's disease
... The present study has shown that the NK cells were phe- notypically altered in BD patients, especially those in inactive disease status. Features of the circulating NK cells in iBD patients included: downregulated gene ...
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The Human Cytomegalovirus Protein UL16 Mediates Increased Resistance to Natural Killer Cell Cytotoxicity through Resistance to Cytolytic Proteins
... the bacterial toxin SLO, and porcine NK lysin (a porcine an- tibacterial peptide), which utilize different mechanisms to in- duce pore formation and to induce apoptosis of target cells. Interestingly, while ...
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Assessment of Markers of the Cell-Mediated Immune Response after Influenza Virus Infection in Frail Older Adults
... the granzyme B assay, in combination with the clinical profile of symptoms in the study subjects, to predict culture-positive versus culture-negative status from na- sopharyngeal swab ...low granzyme ...
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