green fluorescent protein expression
Green fluorescent protein as a marker for Pseudomonas spp.
... GFP expression in different species (de- scribed above) and under various environmental conditions may make it difficult to utilize GFP-derived fluorescence to quantitate cells ...other fluorescent par- ...
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Influence of global gene regulatory networks on single cell heterogeneity of green fluorescent protein production in Bacillus subtilis
... products. Green fluorescent protein (GFP) is commonly used as a marker for determining the strength of a given promoter or for the subcellular localization of a fusion ...GFP expression among ...
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Characterization of serine proteinase expression in agaricus bisporus and coprinopsis cinerea by using green fluorescent protein and the A bisporus SPR1 Promoter
... Foster Article Title: Characterization of Serine Proteinase Expression in Agaricus bisporus and Coprinopsis cinerea by Using Green Fluorescent Protein and the A... Characterisation of se[r] ...
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Highly efficient genetic transduction of primary human synoviocytes with concentrated retroviral supernatant
... the expression of a viral enhanced green fluorescent protein transgene in transduced cells, and by analysis of viral RNA in retroviral ...transgene expression in primary ...
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Intramyocardial transplantation of cardiac mesenchymal stem cells reduces myocarditis in a model of chronic Chagas disease cardiomyopathy
... from green fluorescent protein (GFP) transgenic C57BL/6 mouse hearts and tested for adipogenic, osteogenic, chondrogenic, endothelial, and cardiogenic differentiation potentials evaluated by ...
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Expression and cytosolic assembly of the S-layer fusion protein mSbsC-EGFP in eukaryotic cells
... Upon expression in E. coli the recombinant mSbsC-EGFP fusion protein was recovered from the insoluble ...fusion protein assembled in solution and yielded green fluorescent cylindric ...
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Transient Expression Assay of Aγ-588 (A/G) Mutations in the K562 Cell Line
... of green fluorescent protein (GFP) expression of transfected pEGFP-N1 plasmid (Clontech, Palo Alto, CA, USA) by flow cytometry (Partec, ...
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Temporal patterns in innate immunity parameters in reef-building corals and linkages with local climatic conditions
... Abstract. Extremes in seasonal environmental conditions can significantly impact the health and phys- iological functioning of reef corals, underscoring the need for knowledge of seasonally specific baselines from which ...
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Spatial and temporal localisation of exopolysaccharide gene expression in mucoid and non mucoid Pseudomonas aeruginosa biofilms : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Microbiology, Massey University, Manawatu, New Zealand
... To observe the spatial localisation of cells expressing each EPS gene, this strain will be transformed with a plasmid encoding the expression of the stable green fluorescent protein gfp [r] ...
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Original Article Depleting ABCE1 expression induces apoptosis and inhibits the ability of proliferation and migration of human esophageal carcinoma cells
... The expression of ABCE1 and its target protein-RNase L, were first studied in tumor tissues of esophageal carcinoma and adjacent non-tumor ...siRNA green fluorescent protein (GFP) ...
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Potential utility of eGFP-expressing NOG mice (NOG-EGFP) as a high purity cancer sampling system
... ubiquitous expression of enhanced green fluorescent protein (eGFP) were useful for the clear separation of tumor cells and mouse stromal cells in subcutaneous xenografted tumors by ...
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Inteins—A Focus on the Biotechnological Applications of Splicing Promoting Proteins
... the expression and purification of the green fluorescent protein (GFP) [48], and a modified method is also available using the DnaE split intein from Nostoc puntiforme [49] [50], facilitating ...
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Cytosolic phospholipase A2 regulates Golgi structure and modulates intracellular trafficking of membrane proteins
... Adenoviral expression of cPLA 2 in LLC-PK 1 kidney epithelial cells prevents constitutive trafficking to the plasma membrane of an aquaporin 2- green fluorescent protein chimera, with ...
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Genotype-independent Agrobacterium rhizogenes-mediated root transformation of chickpea: a rapid and efficient method for reverse genetics studies
... Results: Transformation positive roots were obtained on selection medium after two weeks of A. rhizogenes inocula- tion. Expression of green fluorescent protein further confirmed the success ...
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A "humanized" green fluorescent protein cDNA adapted for high-level expression in mammalian cells.
... technologies. Green fluorescent protein (GFP) from the jellyfish Aequorea victoria is a protein of 238 amino acids which absorbs blue light (major peak at 395 nm) and emits green light ...
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Fluoresceination of Lactobacillus rhamnosus through the expression of green fluorescent protein
... GFPmut2 gene was subcloned onto the Lactobacillus-E. coli shuttle vector pRN14. We produced pRNemgfp, which consists of the gfpmut2 gene downstream of the erythromycin resistance gene, erm on pRN14.Culturing in MRS ...
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Enhanced fluorescent properties of an OmpT site deleted mutant of Green Fluorescent Protein
... Random and directed point mutations of gene for GFP have resulted in various changes in its fluorescent behav- iour. These include mutation of Tyr66 in the fluorophore to His66 resulting in a shift of the ...
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High frequency, cell type-specific visualization of fluorescent-tagged genomic sites in interphase and mitotic cells of living Arabidopsis plants
... fusion protein but lacking operator ...that green fluorescent-tagged sites can be visualized on moving mitotic chromosomes stained with red fluorescent-labelled histone ...fusion ...
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Analysis of Adaptive Mutations in Kunjin Virus Replicon RNA Reveals a Novel Role for the Flavivirus Nonstructural Protein NS2A in Inhibition of Beta Interferon Promoter-Driven Transcription
... NS2A protein inhibits activation of IFN-  promoter-driven ...NS2A protein with an Ala30-to-Pro mutation to in- hibit activation of IFN-  promoter-driven transcription, we cloned corresponding gene ...
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New insights into enzymatic hydrolysis of heterogeneous cellulose by using carbohydrate-binding module 3 containing GFP and carbohydrate-binding module 17 containing CFP
... the protein expression plasmid. The GC3 fusion protein containing a GFP and CBM3 (Figure 1A) was pro- duced and purified as described elsewhere ...sion protein was produced by ...CC17 ...
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