Green fluorescent protein (GFP) analysis
Quantitative analysis of gene expression with an improved green fluorescent protein
... only in three base pairs it is unlikely that these alterations influence expression of both genes from the same promoter differently. The fluorescence of cells containing the tetAgfp+ fusion reliably reported promoter ...
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Green fluorescent protein (gfp) mutants
... Materials and methods: Initially DNA is isolated and GFPuv is sub-cloned into the pET28c vector from pET23 plasmid by speectrophotometric analysis. 5µg of pET23GFPuv DNA is digested by using NdeI and HindIII ...
Green Fluorescent Protein as a Targeting Probe.
... trajectory analysis reveals no significant difference in BD_Ser65OH_Glu222O and BD_Tyr66O_Arg96HH21/22 between wild type GFP and none of the RGD and HAV GFP loop ...
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Green Fluorescent Protein-Tagged Retroviral Envelope Protein for Analysis of Virus-Cell Interactions
... Env protein provides virus particles that are detectable by CLSM and can be used to detect virus receptor levels on target ...envelope protein resulted in virus with only 20% of the infectivity of wild-type ...
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FLUORESCENCE QUENCHING ANALYSIS OF HISTIDINE- TAGGED ENHANCED GREEN FLUORESCENT PROTEIN
... Protein denaturation was observed in different concentration of guanidine hydrochloride, after incubation at room temperature for 16 hours. The results of denaturation are shown in Fig. 2. Fig. 2. Fluorescence of ...
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Green fluorescent protein as a marker for Pseudomonas spp.
... GFP-containing plasmids can also be used to mark cells in FIG. 4. Expression of GFP in mixed cultures. Shown is a mixture B. cepacia and P. aeruginosa carrying the GFP-expressing plasmid pSMC2. Cells of both strains can ...
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Live-Cell Analysis of a Green Fluorescent Protein-Tagged Herpes Simplex Virus Infection
... of protein trafficking in several aspects of cell biology, such as endoplasmic reticulum-to-Golgi apparatus transport (22, 26), the cytoskeleton (16, 20, 30), and cell divi- sion (6, ...lapse analysis of ...
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Green Fluorescent Protein (GFP): Genetic Transformation, Synthesis and Purification of the Recombinant Protein
... Green Fluorescent Protein (GFP): Genetic Transformation, Synthesis and Purification of the Recombinant Protein INTRODUCTION Green Fluorescent Protein (GFP) is a novel ...
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GENETIC TRANSFORMATION OF BACTERIA WITH THE GENE FOR GREEN FLUORESCENT PROTEIN (GFP)
... suspensions evenly. Do not press down too firmly or you will gouge the surface of the agar plate. This can complicate both the growth and analysis of the bacteria on the plates. Remember to use a new sterile loop ...
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Functional Analysis of the Human Cytomegalovirus US28 Gene by Insertion Mutagenesis with the Green Fluorescent Protein Gene
... This receptor may therefore give infected cells an improved or novel migratory ability and thereby contribute to virus dissem- ination. In the construction of the HCMV US28 mutant, the GFP was used as a marker that ...
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Green fluorescent protein regulation in the coral Acropora yongei during photoacclimation
... nested analysis of variance (ANOVA) compared the effects of light treatments on GFP, conjugated ubiquitin immunoblots and dinoflagellate ...multivariate analysis of variance (MANOVA) was used to compare the ...
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Incorporation of the Green Fluorescent Protein into the Herpes Simplex Virus Type 1 Capsid
... fusion protein to incorporate this molecule into the virion ...the analysis of HSV-1 biology, in particular, the analysis of virus replication and transport both in cell culture and in the tri- ...
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Green fluorescent protein-based expression screening of membrane proteins in Escherichia coli.
... Discussion In the protocol described in this article, ligation independent cloning into a GFP reporter vector is combined with fluorescence detection to rapidly screen the relative expression of multiple membrane ...
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Detection of chromosomes tagged with green fluorescent protein in live Arabidopsis thaliana plants
... We used homozygous plants from line EL702C, which carries three copies of the T-DNA insert, for this experiment. Forty microliters of solution containing 0.3 mM of Dex and 100 mM IPTG were dropped on each seedling on the ...
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Development of a method for screening short lived proteins using green fluorescent protein
... of protein abundance, with pharmacologic shutoff of protein ...FACS analysis, 22 (73%) are associated with proteins with a half-life of less than 4 ...
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Colonization of Rice Roots by a Green Fluorescent Protein Tagged Isolate of Ustilaginoidea virens
... The green fluorescent protein (GFP) from the jellyfish Aequorea victoria [19] [20] is a widely used reporter protein to facilitate the observation of hyphae on the root surface ...
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A variant of green fluorescent protein exclusively deposited to active intracellular inclusion bodies
... but fluorescent expression motivated us to check the pos- sibility of the formation of active ...DL4 protein was ex- clusively found in insoluble fraction with traces of DL4 in its soluble fraction, whereas ...
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Two Point Mutations Produce Infectious Retrovirus Bearing a Green Fluorescent Protein-SU Fusion Protein
... blot analysis on virus particles purified from 7 ml of each cell supernatant by high-speed centrifuga- tion through a sucrose ...This analysis would also show if the GFP-SU association was compromised, ...
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Analysis of Receptor Usage by Ecotropic Murine Retroviruses, Using Green Fluorescent Protein-Tagged Cationic Amino Acid Transporters
... DISCUSSION In this study, we demonstrated that GFP-tagged mCAT1 was expressed on the cell surface, retaining its ecotropic re- ceptor activity. mCAT1-GFP was colocalized with the Golgi apparatus in the cytoplasm and ...
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Human Cytomegalovirus Labeled with Green Fluorescent Protein for Live Analysis of Intracellular Particle Movements
... live analysis were cultured on 35-mm-diameter glass- bottom dishes (MatTek, Ashland, ...For analysis of virus entry, infectious supernatant containing green fluorescent virus was prepared ...
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