GTPase-activating protein
The Jaw of the Worm: GTPase-activating Protein EAT-17 Regulates Grinder Formation in Caenorhabditis elegans
... new GTPase-activating protein gene in ...G protein regulating membrane trafficking from Golgi (Del Nery et ...GAP protein EAT-17 show their most striking phenotypes in the grinder, ...
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The Rgd1p Rho GTPase-Activating Protein and the Mid2p Cell Wall Sensor Are Required at Low pH for Protein Kinase C Pathway Activation and Cell Survival in Saccharomyces cerevisiae
... interacts with RGD1. MID2 deletion exacerbates the rgd1 ⌬ strain mortality observed in stationary phase, whereas its over- expression suppresses the rgd1 ⌬ growth defect. In fact, the RGD1 gene, which encodes the ...
12
Conidial Morphogenesis and Septin-Mediated Plant Infection Require Smo1, a Ras GTPase-Activating Protein in Magnaporthe oryzae
... ABSTRACT The pathogenic life cycle of the rice blast fungus Magnaporthe oryzae involves a series of morphogenetic changes, essential for its ability to cause disease. The smo mutation was identi fi ed . 25 years ago, and ...
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C elegans Rab GTPase activating protein TBC 2 promotes cell corpse degradation by regulating the small GTPase RAB 5
... Rab GTPase activating protein (GAP) and its loss of function affects several events of phagosome maturation, including RAB-5 release, phosphatidylinositol 3-phosphate dynamics, phagosomal ...
11
Hyphal Guidance and Invasive Growth in Candida albicans Require the Ras-Like GTPase Rsr1p and Its GTPase-Activating Protein Bud2p
... the GTPase-activating protein (GAP) for ...Rsr1p GTPase, the almost identical phenotypes of the rsr1/rsr1 and bud2/bud2 strains suggest that cycling of Rsr1p between GDP- and GTP- bound states ...
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Ras GTPase-Activating Protein Gap1 of the Homobasidiomycete Schizophyllum commune Regulates Hyphal Growth Orientation and Sexual Development
... The white rot fungus Schizophyllum commune is used for the analysis of mating and sexual development in homobasidiomycete fungi. In this study, we isolated the gene gap1 encoding a GTPase-activating ...
13
Involvement of Ras GTPase-activating protein SH3 domain-binding protein 1in the epithelial-to-mesenchymal transition-induced metastasis of breast cancer cells via the Smad signaling pathway
... Ras GTPase-activating protein SH3 domain-binding protein 1 (G3BP1), an essential Ras mediator, has been implicated in cancer development, including cell growth, motility, invasion and ...
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Ras GTPase-Activating Protein Regulation of Actin Cytoskeleton and Hyphal Polarity in Aspergillus nidulans
... Ras GTPase-activating ...fluorescent protein shows cortical localization with strong predominance at the hyphal tips, we propose that GapA-mediated downregulation of Ras signaling at the plasma ...
13
Identification of IQ motif–containing GTPase-activating protein 1 as a regulator of long-term ketosis
... Adenovirus production and cell culture. HepG2 cell line was obtained from ATCC (catalog HB-8065) and cultured according to ATCC specifications. This cell line tested negative for mycoplasma (Biotool, cata- log B39032). ...
15
Isolation and characterisation of α2-chimaerin cDNA encoding a p21rac GTPase-activating protein with an SH2 domain
... GST-fusion protein that bound phosphoamino acid-coupled agarose beads, to show the binding of the GST/Abl-SH2 domain to phosphotyrosine ...fusion protein was used as a ...
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Rho GTPase activating protein 18: a biomarker associated with good prognosis in invasive breast cancer
... Studies exploring the impact of tissue-based ARHGAP18 protein expression in cancers including BC are lacking and most of the studies so far were performed using in vitro tissue culture platform. In the current ...
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The Arf GTPase Activating Protein Family Is Exploited by Salmonella enterica Serovar Typhimurium To Invade Nonphagocytic Host Cells
... FIG 5 Arf GAP disassembly of Arf1-protein complexes at the membrane. (A) Arf1 interaction with the membrane in the presence of GAPs of Arf6 and Arf1. Silica beads coated with phospholipid bilayers were anchored ...
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Hepatitis C Virus Co-Opts Ras-GTPase-Activating Protein-Binding Protein 1 for Its Genome Replication
... Reduction of G3BP1 levels reduces HCV replication and infection. Since G3BP1 is localized to HCV RCs, we wondered whether it modulates RC formation or viral RNA replication. To address this, we examined HCV replication ...
9
Opposite Roles of the F-Box Protein Rcy1p and the GTPase-Activating Protein Gyp2p During Recycling of Internalized Proteins in Yeast
... F-box protein Rcy1p is part of a non-SCF (Skp1p-cullin-F-box protein) complex involved in recycling of internalized ...F-box protein Rcy1p may activate the Ypt6p GTPase module during ...
10
Control of Polarized Growth by the Rho Family GTPase Rho4 in Budding Yeast: Requirement of the N-Terminal Extension of Rho4 and Regulation by the Rho GTPase-Activating Protein Bem2
... R ho family GTPases are small GTP-binding proteins of the Ras superfamily widespread in eukaryotes from yeasts to humans. They are widely implicated in cellular processes, including cyto- skeletal reorganization, ...
10
Secretory Pathway-Dependent Localization of the Saccharomyces cerevisiae Rho GTPase-Activating Protein Rgd1p at Growth Sites
... We tested if Rgd1p colocalized with secretory vesicles. To do so, a strain was constructed in which Rgd1p was tagged with three copies of GFP and the post-Golgi marker Sec2p was tagged with the DsRed variant tdimer (2). ...
11
Tfs1p, a Member of the PEBP Family, Inhibits the Ira2p but Not the Ira1p Ras GTPase-Activating Protein in Saccharomyces cerevisiae
... Biochemical and structural analyses have shown that the most important function of GAPs is to supply the active site of Ras with an arginine (the finger) localized at the tip of a loop (the finger loop) joining two ...
12
Regulation and structure/function relationships of the Rac1 GTPase-activating protein, n-chimaerin
... by activating adenyl cyclase in the olfactory epithelium hence promoting electrical excitation of olfactory ...dependent protein kinase (PKA) which is composed of the cAMP-binding regulatory dimer and two ...
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Purification and functional characterization of brain and recombinant chimaerin, a p21rac GTPase activating protein
... (lane 7 in Fig. 4A). p100 and p150 GAPs were enriched in the brain cortex and olfactory bulbs (lanes 2 and 3 in Fig. 4A). Several darker bands of about 60-65 kDa were detected on the ra d overlay that appeared as white ...
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Lrg1p Is a Rho1 GTPase-Activating Protein Required for Efficient Cell Fusion in Yeast
... deposition. In the lrg1 and fus2 mutants, the entire sur- face of the shmoo projection was stained, including the tip. In the lrg1 mutant, overall staining of the cell was distinctly brighter than that of the wild-type ...
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