GTPase-activating protein (GAP)
Hyphal Guidance and Invasive Growth in Candida albicans Require the Ras-Like GTPase Rsr1p and Its GTPase-Activating Protein Bud2p
... the GTPase-activating protein (GAP) for ...the GAP for ...Rsr1p GTPase, the almost identical phenotypes of the rsr1/rsr1 and bud2/bud2 strains suggest that cycling of Rsr1p ...
14
The Jaw of the Worm: GTPase-activating Protein EAT-17 Regulates Grinder Formation in Caenorhabditis elegans
... new GTPase-activating protein gene in ...G protein regulating membrane trafficking from Golgi (Del Nery et ...specific GAP for ...its GAP protein EAT-17 show their most ...
21
C elegans Rab GTPase activating protein TBC 2 promotes cell corpse degradation by regulating the small GTPase RAB 5
... Rab GTPase activating protein (GAP) and its loss of function affects several events of phagosome maturation, including RAB-5 release, phosphatidylinositol 3-phosphate dynamics, phagosomal ...
11
Ras GTPase-Activating Protein Gap1 of the Homobasidiomycete Schizophyllum commune Regulates Hyphal Growth Orientation and Sexual Development
... The white rot fungus Schizophyllum commune is used for the analysis of mating and sexual development in homobasidiomycete fungi. In this study, we isolated the gene gap1 encoding a GTPase-activating ...
13
Ras GTPase-Activating Protein Regulation of Actin Cytoskeleton and Hyphal Polarity in Aspergillus nidulans
... Ras GTPase-activating ...fluorescent protein shows cortical localization with strong predominance at the hyphal tips, we propose that GapA-mediated downregulation of Ras signaling at the plasma ...
13
Conidial Morphogenesis and Septin-Mediated Plant Infection Require Smo1, a Ras GTPase-Activating Protein in Magnaporthe oryzae
... ABSTRACT The pathogenic life cycle of the rice blast fungus Magnaporthe oryzae involves a series of morphogenetic changes, essential for its ability to cause disease. The smo mutation was identi fi ed . 25 years ago, and ...
17
Tfs1p, a Member of the PEBP Family, Inhibits the Ira2p but Not the Ira1p Ras GTPase-Activating Protein in Saccharomyces cerevisiae
... proteins: GTPase-activating proteins (GAPs) inhibit Ras proteins, whereas guanine exchange factors activate ...Ras GAP, Ira2p, in Saccharomyces ...phosphatidylethanolamine-binding protein ...
12
Lrg1p Is a Rho1 GTPase-Activating Protein Required for Efficient Cell Fusion in Yeast
... Lrg1p protein is depicted ...for Protein Sequences ...Rho GAP homology domain (755–910 aa) are represented by the open ...SWISS-PROT Protein Database also predicts LIM1, LIM2, LIM4, and the ...
14
Purification and functional characterization of brain and recombinant chimaerin, a p21rac GTPase activating protein
... brain protein of 45 kDa was usually detected by western assay using anti-chimaerin ...the GAP overlay assay involved separating proteins by SDS-PAGE followed by western ...(and GAP activity) was ...
243
Isolation and characterisation of α2-chimaerin cDNA encoding a p21rac GTPase-activating protein with an SH2 domain
... induce GAP to complex with two other tyrosine phosphorylated proteins (p62 and pl90) ...a protein that has extensive sequence similarity to a putative heterogeneous nuclear RNP protein, GRP33; it ...
263
Opposite Roles of the F-Box Protein Rcy1p and the GTPase-Activating Protein Gyp2p During Recycling of Internalized Proteins in Yeast
... F-box protein Rcy1p is part of a non-SCF (Skp1p-cullin-F-box protein) complex involved in recycling of internalized ...the GAP Gyp2p restores recycling in rcy1⌬, while overexpression of an active ...
10
The Arf GTPase Activating Protein Family Is Exploited by Salmonella enterica Serovar Typhimurium To Invade Nonphagocytic Host Cells
... Arf GAP family members play a role in Salmonella-induced mem- brane ruffling, we examined their localization during infection of Caco intestinal epithelial cells expressing fluorescent representa- tives from each ...
11
The Arf-GTPase-Activating Protein Gcs1p Is Essential for Sporulation and Regulates the Phospholipase D Spo14p
... SPO14, encoding the major Saccharomyces cerevisiae phospholipase D (PLD), is essential for sporulation and mediates synthesis of the new membrane that encompasses the haploid nuclei that arise through meiotic divisions. ...
13
Control of Polarized Growth by the Rho Family GTPase Rho4 in Budding Yeast: Requirement of the N-Terminal Extension of Rho4 and Regulation by the Rho GTPase-Activating Protein Bem2
... Isolation of temperature-sensitive rho3 ⌬ rho4-Ts mutants. RHO4 was randomly mutagenized by an error-prone PCR method (28). The PCR-amplified RHO4 genes with mutations were mixed with BamHI- and HindIII-digested plasmid ...
10
Rho GTPase activating protein 18: a biomarker associated with good prognosis in invasive breast cancer
... ARHGAP18 protein, confirming the specificity of the antibody, Figure ...ARHGAP18 protein was localised in the nuclei and cytoplasm of tumour cells with varying intensities, Figure 1B, C ...
29
Hepatitis C Virus Co-Opts Ras-GTPase-Activating Protein-Binding Protein 1 for Its Genome Replication
... Reduction of G3BP1 levels reduces HCV replication and infection. Since G3BP1 is localized to HCV RCs, we wondered whether it modulates RC formation or viral RNA replication. To address this, we examined HCV replication ...
9
Identification of IQ motif–containing GTPase-activating protein 1 as a regulator of long-term ketosis
... at ZT4 following a 24-hour fast and were sacrificed 2 hours later at ZT6 (n = 6–9 mice per genotype). An additional cohort of RF mice was injected with 10 mg/kg rapamycin intraperitoneally 1 hour prior to RF (n = 6–13 ...
15
Secretory Pathway-Dependent Localization of the Saccharomyces cerevisiae Rho GTPase-Activating Protein Rgd1p at Growth Sites
... The GTPase Rho1p was found to be localized to the Golgi apparatus and post-Golgi vesicles, and its localization is disrupted in mutants acting at late steps of the se- FIG 5 Colocalization of Rgd1p with Sec2p, a ...
11
RPE phagocytic function declines in age related macular degeneration and is rescued by human umbilical tissue derived cells
... Rho GTPase activating protein 9; ATCC : American Type Culture Collection; ATP: adenosine triphosphate; BDNF: brain-derived neuro- trophic factor; CM: conditioned media; DMEM: Dulbecco’s modified ...
15
Lymphangioleiomyomatosis — a wolf in sheep’s clothing
... conserved GTPase-activating protein (GAP), and TSC1 encodes hamartin, which heterodimerizes with tuberin (49) and appears to be essential for its function (Figure ...regulates protein ...
11