GTPase per concentration for wild-type protein
A Rho-Type GTPase, rho-4, Is Required for Septation in Neurospora crassa
... Immunofluorescence and fluorescence microscopy. Conidia or mycelia were inoculated onto slides covered in thin layers of Vogel’s minimal medium (MM) or inoculated directly onto petri plates with Vogel’s MM solidified ...
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Effect of Cleavage Mutants on Syncytium Formation Directed by the Wild-Type Fusion Protein of Newcastle Disease Virus
... fully wild-type oligomers as well as full or partial activity of some ...F protein was proportional to the input DNA and that the ex- pression levels of wild-type and mutant DNAs were ...
7
Random mutagenesis of NS1 protein of influenza a H1N1 and docking of RNA aptamers to wild type and mutant NS1 proteins
... increased concentration of MgCl 2 to 10 mM and subsequently cloned into yT&A vector and transformed into ...the wild-type protein were modeled using EasyModeller ...the wild-type ...
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HSC70 is a chaperone for wild-type and mutant cardiac myosin binding protein C
... fields per coverslip per condition were acquired with a ×60 objective using a Nikon Eclipse Ti-E inverted fluorescence microscope under single-blind ...
17
Wild-Type Levels of Human Immunodeficiency Virus Type 1 Infectivity in the Absence of Cellular Emerin Protein
... emerin protein revealed a relatively minor ( ⬃30%) infec- tion defect ...emerin protein in determining HIV-1 infectivity in human MDM, it remains possible that greater knockdowns would unveil more ...
7
Viral Adaptation to an Antiviral Protein Enhances the Fitness Level to Above That of the Uninhibited Wild Type
... coat protein resistance mutations may produce early assembly intermediates that are less prone to associate with scaffolding subunits, both wild type and ...small concentration of the more ...
5
Hyphal Guidance and Invasive Growth in Candida albicans Require the Ras-Like GTPase Rsr1p and Its GTPase-Activating Protein Bud2p
... Time-lapse morphological analyses. Yeast cells were cultured in SDC medium with uridine overnight at 28°C and then subcultured (1:20) into fresh medium with 10% serum, prewarmed to 37°C. After 5 min at 37°C, 200 l of ...
14
Hemagglutinin Protein of Wild-Type Measles Virus Activates Toll-Like Receptor 2 Signaling
... H protein is the viral component triggering both human and mouse TLR2 activation, and this property is abolished upon a single amino acid ...glycoprotein concentration of the individual viruses was adjusted ...
8
C elegans Rab GTPase activating protein TBC 2 promotes cell corpse degradation by regulating the small GTPase RAB 5
... phagosomal recruitment of RAB-7 and LMP-1 was similarly reduced in qxIs157 animals, as only 44% and 25% of cell corpses were positive for GFP::RAB-7 and LMP-1::GFP, respectively, compared with 74% and 65% in ...
11
Expression and Properties of Wild-Type and Mutant Forms of the Drosophila Sex Comb on Midleg (SCM) Repressor Protein
... complexes. In this study, we compared how heterozygos- notypes that are sensitive indicators of PcG function ( Jurgens 1985; Wu et al. 1989; M. Muller and H. Gyur- ity for different Scm mutations affects PcG function in ...
12
Lrg1p Is a Rho1 GTPase-Activating Protein Required for Efficient Cell Fusion in Yeast
... the wild-type strain, con- sistent with a role for Lrg1p as a negative regulator of ...of wild- type FUS2 also restored the normal staining pattern, whereas the empty vector had no ...In ...
14
al.. The GTPase-activating protein-related domain of neurofibromin interacts with MC1R and regulates
... Analysis of the impact of GRD mutations on GRD/MC1R interaction. We then explored whether this interaction might be affected in pathologic conditions. We assessed whether mutations in GRD, i.e. R1276P identified in NF1 ...
21
The SH3/PH Domain Protein AgBoi1/2 Collaborates with the Rho-Type GTPase AgRho3 To Prevent Nonpolar Growth at Hyphal Tips of Ashbya gossypii
... of wild-type hyphae with latrunculin ...A wild-type spore at ...The wild type (c) establishes two polar growth sites from the germ bubble in the first 4 h and initiates lateral ...
13
Protein wild-type and mutant ensemble database
... ABSTRACT Protein structures have been determined and deposited into Protein Data Bank at an in- creasing ...the protein structures in the PDB and form a wild type and mutant structure ...
35
Wild-Type Measles Virus with the Hemagglutinin Protein of the Edmonston Vaccine Strain Retains Wild-Type Tropism in Macaques
... and wild-type strains of measles virus (MV) in vitro is the wider cell specificity of vaccine strains, resulting from the receptor usage of the hemagglutinin (H) ...protein. Wild-type H ...
11
Characterization of a Brain-specific Rho GTPase-activating Protein, p200rhogap*
... Rho GTPase-activating proteins (RhoGAPs) are a family of multifunctional molecules that transduce diverse intracellular signals by regulating Rho GTPase ...the GTPase activities of Rac1 and RhoA in ...
9
Receptor compaction and GTPase movements drive cotranslational protein translocation
... of protein translocation at the ER is not well ...SRP•SR GTPase complex from the vicinity of the ribosome exit, where it initially assembles, to the distal site of ...cotranslational protein ...
27
Cellular localization of the transforming protein of wild-type and temperature-sensitive Fujinami sarcoma virus.
... In cells infected by mutants of FSV temperature sensitive for cellular transformation, most of the P140 is particulate at the permissive tempera- ture, whereas most is soluble at the non[r] ...
9
Localization of a red fluorescence protein adsorbed on wild type and mutant spores of Bacillus subtilis
... In order to verify whether mRFP molecules infiltrate inside the spore we used a fluorescence microscopy approach (“Methods” section). In particular we followed the red fluorescence of mRFP and the green fluorescence of ...
12
Assays for Determination of Protein Concentration
... The protocols in this unit should produce valid results for most applications. There are also kits available for protein determination from Sigma, BioRad, and Pierce. Prior to employing an assay kit or protocol, ...
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