Haemophilus ducreyi
Simultaneous PCR detection of Haemophilus ducreyi, Treponema pallidum, and herpes simplex virus types 1 and 2 from genital ulcers
... and Haemophilus ducreyi (the causative agent of ...H. ducreyi cultures are performed in very few clinical laboratories and dark-field examinations are not done in many clinical settings ...
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The Haemophilus ducreyi LspA1 Protein Inhibits Phagocytosis By Using a New Mechanism Involving Activation of C Terminal Src Kinase
... ABSTRACT Haemophilus ducreyi causes chancroid, a sexually transmitted ...H. ducreyi inhibit phagocytosis by stimulating the catalytic activity of C-terminal Src kinase (Csk), which itself inhibits ...
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The cytolethal distending toxin from the chancroid bacterium Haemophilus ducreyi induces cell cycle arrest in the G2 phase
... The potent cytolethal distending toxin produced by Haemophilus ducreyi is a putative virulence factor in the pathogenesis of chancroid. We studied its action on eukaryotic cells, with the long-term goal of ...
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Haemophilus ducreyi Hfq Contributes to Virulence Gene Regulation as Cells Enter Stationary Phase
... of Haemophilus ducreyi, which lacks an RpoS ...H. ducreyi to form microcolonies, downregulated DsrA and rendered H. ducreyi serum susceptible, and downregu- lated LspB and LspA2, which allow ...
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Clear broth and plate media for culture of Haemophilus ducreyi
... Using catalase as a source of heme, we have developed both clear plate and broth media for the growth of Haemophilus ducreyi, the causative agent of chancroid.. In the broth medium, the [r] ...
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Etiology of Genital Ulcer Disease in Dakar, Senegal, and Comparison of PCR and Serologic Assays for Detection of Haemophilus ducreyi
... current Haemophilus ducreyi ...H. ducreyi, ...H. ducreyi DNA was detected in 60% (3 of 5) of samples from ulcerated ...H. ducreyi PCR assays were 83% concordant, with the recD PCR assay ...
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Development of a Rapid Immunodiagnostic Test for Haemophilus ducreyi
... Haemophilus ducreyi is the etiologic agent of chancroid, a sexually transmitted disease that increases the rate of transmission of human immunodeficiency ...H. ducreyi, ...H. ducreyi tested ...
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Molecular Characterization of Haemophilus ducreyi Isolates from Different Geographical Locations
... The OPJ20 primer was more discriminatory: nine RAPD profiles were obtained (Fig. 2) and were designated patterns 1 to 9 (Table 2). The three reference strains included in this study revealed a different pattern each, 1, ...
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Comparison of specimen collection and laboratory techniques for isolation of Haemophilus ducreyi
... in the past by Gram stain of genital pus 1, 6, ducreyi to several antibiotics, using a disk susby the chaining morphology of the organism in ceptibility technique, showed that these isol[r] ...
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Effectors of Haemophilus ducreyi pathogenesis
... H. ducreyi mutant expressing a modified “double mutant” CdtB ...H. ducreyi DMB (35000 dmcdtB) are as toxigenic as wild type to cultured cells and that in vitro nuclease activity of recombinant dmCdtB is ...
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Standardization of an enzyme immunoassay for human antibody to Haemophilus ducreyi
... The performance of the EIA was compared by using the larger group of positive case serum specimens from patients with chancroid with respect to the time of phlebotomy from the time of on[r] ...
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Isolation and cultivation of Haemophilus ducreyi
... Gram stain characteristics taken from blood clot tubes and solid media, cellular and colonial morphology of the bacilli, and lack of oxidase, catalase, and biochemical activity except ni[r] ...
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Molecular characterization of Haemophilus ducreyi by ribosomal DNA fingerprinting
... The utility of ribotyping as a tool to study the epidemiology of chancroid was further evaluated by comparing the ribotypes and plasmid profiles for 18 isolates from Orange County, Calif[r] ...
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DNA probes for the identification of Haemophilus ducreyi
... Nine isolates, six belonging to other Haemophilus species and three belonging to Pasteurella species, reacted weakly with the probes when approximately 3.0 x 107 to 6.0 x 107 CFU was tes[r] ...
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Isolation and identification of Haemophilus ducreyi in a clinical study
... Media used for primary isolation at various times during the study were enriched chocolate agar, chocolate agar plus vancomycin 3 yig/ml, rabbit blood agar plus vancomycin 3 ,ug/ml, feta[r] ...
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Protection of Piglets by a Haemophilus parasuis Ghost Vaccine against Homologous Challenge
... by Haemophilus parasuis; however, the protective efficacy varies depending on the strain and ...the Haemophilus parasuis serovar 5 reference strain Nagasaki was prepared and used to inoculate ...
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Dual RNA seq of Nontypeable Haemophilus influenzae and Host Cell Transcriptomes Reveals Novel Insights into Host Pathogen Cross Talk
... Transcriptome signatures highlight progression of infec- tion. (i) Bacterial stress response elements are activated at late stages of infection. Under the experimental conditions used, we observed an increased number of ...
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A high throughput serum bactericidal assay for antibodies to Haemophilus influenzae type b
... Background: The protective capacities of antibodies induced with Haemophilus influenzae type b (Hib) vaccines can be directly assessed in vitro with a Hib-specific serum bactericidal assay (SBA). However, the ...
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Case report of congenital asplenia presenting with Haemophilus influenzae type a (Hia) sepsis: an emerging pediatric infection in Minnesota
... A surprising finding in our case was the discovery of Howell-Jolly bodies on a peripheral blood smear at the time of our patient’s initial presentation in septic shock. This was followed by ultrasonographic confirmation ...
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Coinfection with Haemophilus parasuis serovar 4 increases the virulence of porcine circovirus type 2 in piglets
... A positively synergistic interaction effect on the mRNA expression of CD14, TLR4 and TLR8 might enhance the production of pro-inflammatory cytokines such as IL-1β [41]. Experimental exposure of pigs to LPS following ...
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