HBV DNA
Assessment of the Target Capture PCR Hepatitis B Virus (HBV) DNA Quantitative Assay and Comparison with Commercial HBV DNA Quantitative Assays
... PCR HBV DNA quantitative ...nucleotide HBV internal control (IC) single-stranded DNA, modified by re- placement with the probe binding sequence, was prepared as a control for target isolation ...
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COBAS AmpliPrep COBAS TaqMan Hepatitis B Virus (HBV) Test: a Novel Automated Real Time PCR Assay for Quantification of HBV DNA in Plasma
... an HBV DNA automated extraction based on the affinity of DNA for silica gel-covered magnetic ...K, DNA capture by use of glass particles, and purification. After DNA elution at high ...
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Anti HBc and HBV DNA detection in blood donors negative for hepatitis B virus surface antigen
... Occult HBV infection is defined as the presence of hepatitis B virus (HBV) DNA in blood or liver tissues in patients negative for Hepatitis B surface Antigen ...of HBV DNA in the serum ...
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Dynamic changes of HBV markers and HBV DNA load in infants born to HBsAg(+) mothers: can positivity of HBsAg or HBV DNA at birth be an indicator for HBV infection of infants?
... tected HBV markers positive, following 12 months follow- up, we found that the positive rates of HBV DNA and HBsAg gradually ...or HBV DNA(+) at birth resolved after 12 months follow-up ...
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Phosphoacceptors Threonine 162 and Serines 170 and 178 within the Carboxyl-Terminal RRRS/T Motif of the Hepatitis B Virus Core Protein Make Multiple Contributions to Hepatitis B Virus Replication
... Impaired HBV DNA synthesis and absence of RC DNA in the presence of mutant C ...on HBV replication, we analyzed HBV DNA synthesis by Southern blotting of Huh7 cells cotransfected ...
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Enhancement of Hepatitis B Virus Replication by Androgen and Its Receptor in Mice
... 1.3-mer HBV genomes with and without these ARE mutations were first transfected into Huh7 hepatoma cells for an analysis of their response to the androgen agonist ...wild-type HBV RI DNA and RNA ...
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Original Article Clinical efficacy of tenofovir and entecavir in the treatment of chronic hepatitis B infection in patients naïve to nucleosides and their analogues therapy
... inhibit HBV replication and alleviate hepatic fibrosis or cirrhosis [14, ...suppress HBV replication in both HBeAg- positive and -negative patients ...inhibit HBV replication, so that it can be used ...
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Correlation of HBcrAg with Intrahepatic Hepatitis B Virus Total DNA and Covalently Closed Circular DNA in HBeAg Positive Chronic Hepatitis B Patients
... between HBV parameters (23, ...intrahepatic HBV cccDNA and the distribution of intrahepatic HBV cccDNA and HBV DNA values were different, which might be related to the inconsistent ...
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Characterization of a New Sensitive PCR Assay for Quantification of Viral DNA Isolated from Patients with Hepatitis B Virus Infections
... RealTime HBV assay meets all requirements for use in monitoring viral response to antiviral ...of HBV DNA at levels well below the levels considered necessary for the control of disease progression ...
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Activation and Inhibition of Cellular Calcium and Tyrosine Kinase Signaling Pathways Identify Targets of the HBx Protein Involved in Hepatitis B Virus Replication
... diprovoxil-resistant HBV mutants is not established, but results to date look promising, although resis- tance is always possible for viruses that replicate through re- verse ...likely. HBV is the prototype ...
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Inhibitory effect of Phyllanthus urinaria L extract on the replication of lamivudine resistant hepatitis B virus in vitro
... of HBV P protein YMDD motif mutants The conserved 203 YMDD 206 motif within the RT cata- lytic C sub-domain of the HBV P protein is responsible for RNA- and DNA-dependent DNA polymerase activ- ...
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Human APOBEC1 cytidine deaminase edits HBV DNA
... on HBV replication using 3DPCR ...of HBV was transfected into quail QT6 cells with hA1 along with human APOBEC2 (hA2) and human APOBEC3G (hA3G) as negative and positive con- ...of HBV DNA, ...
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Regulation of Hepatitis B Virus Replication by the Ras-Mitogen-Activated Protein Kinase Signaling Pathway
... of HBV can be regulated by a variety of external stimuli, includ- ing hormones, growth factors, cytokines, and the cell cycle (5, 12, 14, 29, 30, ...presses HBV DNA replication in a dose-dependent ...
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Deamination-Independent Inhibition of Hepatitis B Virus Reverse Transcription by APOBEC3G
... of HBV core ...inhibit HBV replication, the human hepatoma cell line HepG2 was cotransfected with HBV- and APOBEC3- expressing plasmids and HBV DNA levels in the cytoplasmic ...
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Serum Hepatitis B Virus DNA, RNA, and HBsAg: Which Correlated Better with Intrahepatic Covalently Closed Circular DNA before and after Nucleos(t)ide Analogue Treatment?
... serum HBV DNA levels of ⬎ 300 copies/ml at week 24 or for whom virological breakthrough occurred during the 96-week ...serum HBV DNA, HBsAg, HBeAg, and ALT levels were ...for HBV RNA ...
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Fully Automated, Internally Controlled Quantification of Hepatitis B Virus DNA by Real Time PCR by Use of the MagNA Pure LC and LightCycler Instruments
... Start DNA Master Hybridization Probes (Roche Diagnostics ...mM), HBV DNA-specific primers (0.4 M each), and the HBV DNA- and IC DNA-specific FRET hybridization probes ...to ...
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Associations between HLA Class I Alleles and Escape Mutations in the Hepatitis B Virus Core Gene in New Zealand-Resident Tongans
... of HBV. HBV DNA was extracted from 300 l of serum using the High Pure Viral Nucleic Acid kit (Roche Diagnostics, India- napolis, ...the HBV in the Tongan ...the HBV from each subject, ...
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Clinical Evaluation of the Digene Hybrid Capture II Test and the COBAS AMPLICOR Monitor Test for Determination of Hepatitis B Virus DNA Levels
... the HBV DNA levels obtained by the Digene HC II assay were lower than those obtained by the COBAS-AM ...higher HBV DNA levels, these findings suggested that the predilution procedure for the ...
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Effect of dipterinyl calcium pentahydrate on hepatitis B virus replication in transgenic mice
... liver HBV DNA (PCR) was calculated for each mouse based upon the DCP-EM regression from Table 1, Table 2, rearranged algebrai- cally as shown in the legend of Figure ...liver HBV DNA (PCR) ...
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In Vitro Study of the Effects of Precore and Lamivudine-Resistant Mutations on Hepatitis B Virus Replication
... to HBV SS DNA was detectable at day 1 p.i., and HBV DS DNA became ap- parent by 2 days ...wt HBV recombinant baculovirus (2, 13). The levels of PC HBV RI were greater than wt RI ...
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