HCT 116
combined with 5-fluorouracil on HCT-116 cell
... Abstract: 5-FU is widely used for the treatment of colorectal cancer (CRC), however, the severe cells toxicity of 5-FU is a principal obstacle of clinical application in CRC. 20(R)-ginsenoside Rg 3 (20-(R)-Rg 3 , GRg 3 ...
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Synthesis and Anticancer Effect of 3,4,5-N-Alkyl-Benzamides on Colon Carcinoma HCT- 116 Cells
... gallic acid as an original compound and doxorubicine as a positive control.Amidation of gallic acid with six corresponding alkylamines gave desired -N-methyl-, -N-ethyl-, -N-butyl-, -N-sec-butyl-, -N-ters-butyl-, and ...
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Investigations on Apoptotic Activities of Cherry Laurel Extracts in HCT-116 Human Colon Carcinoma Cells
... In Figure 5, the extent of cells which become late apop- totic or necrotic were demonstrated. At relatively low concentrations (mostly 2-6% extract), the extent of late apoptotic or necrotic cells appeared to be a ...
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Anticancer effect of gemcitabine combined with 5 flurouracil in microemulsion on the hct 116 colon cancer cells
... Combination therapies of chemotherapeutic agents in nanoparticelsare promising strategy in releasing the drugs selectively into the targeted tissues and cells. 5Flurouracil (5FU) and Gemcitabine (GEM) are ...
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Phytochemical Analysis and Anticancer Activity of Seaweed Gracilaria Verrucosa Against Colorectal HCT-116 Cells
... In this research, we develop Indonesia marine resource of seaweed Gracilaria verrucosa as an anti-colorectal cancer agent. Seaweed Gracilaria verrucosa which was collected from east Lombok beach, Nusa Tenggara Barat, ...
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Phytochemistry profile and in vitro cytotoxicity of seaweed macroalgae Sargassum polycystum against colon HCT-116 and lung A-549 cancer cells
... colon HCT-116 and lung-A549 cancer cells by MTT cell proliferation ...colon HCT-116 and lung-A549 cells with median inhibitory concentration ranging from ...
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IN VITRO ASSESSMENT OF GARLIC EXTRACT AND PURIFIED ALLICIN AGAINST LIVER (HEPG2) AND COLORECTAL (HCT-116) CANCER CELL LINES
... Cancer is one of the major public burden worldwide. It is a multicellular disease that can arise from all cell type. In the recent decades, the number of cancer related showed a clear elevation, in turn creating huge ...
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Quince Polysaccharides Induced Apoptosis in Human Colon Cancer Cells (HCT-116)
... RNA isolation and quantitative real-time RT-PCR analysis Total RNA were extracted from HCT-116 cells with TRIzol Reagent following the manufacturer's instruction. The RNA quality was verified using ...
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Resistin and Visfatin Expression in HCT-116 Colorectal Cancer Cell Line
... method and confirmed by RT-PCR, Northern analysis and in situ hybridization in 1999 (35). However, the cell responsible for its secretion has not been definitely identified. The subsequent study on colorectal cancer ...
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Inhibition of 6-phosphofructo-2-kinase (PFKFB3) induces autophagy as a survival mechanism
... Intracellular F2,6BP levels were determined using a method previously described [27]. Briefly, HCT-116 cells were har- vested 48 hours after transfection or after treatment with 3PO and centrifuged at 200 ...
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ANTITUMOR ACTIVITY OF POTENT FRACTION OF LAWSONIA INERMIS ROOT EXTRACT BY IN VITRO AND IN VIVO MODELS
... In the present scenario, products derived from plants serve as a potential source of anticancer drugs with low toxicity and cost. Lawsonia inermis (LI) has been selected to investigate its antitumor activity due to the ...
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Sema3E–Plexin D1 signaling drives human cancer cell invasiveness and metastatic spreading in mice
... In order to assess the functional relevance of endogenous Sema3E expression in tumor cells, we furthermore generated and transplanted in vivo Sema3E-deficient A549 cells (Figure 2, D–F). Analogously, we knocked down ...
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A facile synthesis, and antimicrobial and anticancer activities of some pyridines, thioamides, thiazole, urea, quinazoline, β-naphthyl carbamate, and pyrano[2,3-d]thiazole derivatives
... of HCT-116 on human cancer cell lines against Streptococcus pneumonia and Bacil- lus subtilis as examples of Gram-positive bacteria and Pseudomonas aeruginosa and Escherichia coli as exam- ples of ...
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A-kinase anchor protein 4 (AKAP4) a promising therapeutic target of colorectal cancer
... We were intrigued by our Western blot results analysis which showed the effects of ablation of AKAP4 protein in COLO 205 and HCT 116 on various signaling mole- cules Figs. 2b, 3b and 4b. Therefore, we next ...
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Drosophila homologue of Diaphanous 1 (DIAPH1) controls the metastatic potential of colon cancer cells by regulating microtubule-dependent adhesion
... Figure 5: Effects of DIAPH1-depletion in HCT-116 cells on cytoskeletal dynamics, cell spreading and formation of focal adhesions Fas. A. Scrambled control cells were treated with DMSO (control), 1 μM ...
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LncRNA CASC9 interacts with CPSF3 to regulate TGF-β signaling in colorectal cancer
... The observations that CPSF3 has an effect similar to that of CASC9 in CRC cells and that CPSF3 is a specific interaction partner of CASC9 led us to hypothesize that suppression of CPSF3 would induce regulatory changes ...
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A New Dolabrane Dinorditerpene from Ceriops tagal
... (CNE-2, HCT-116, HepG2and A549) were also evaluated, which displayed that only compound 2 had significant cytotoxicity against those cell lines with IC50 values of ...
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Oroxylin A modulates mitochondrial function and apoptosis in human colon cancer cells by inducing mitochondrial translocation of wild-type p53
... HCT-116 and CaCo-2 cells were seeded in 96-well plates, incubated overnight, and the treated with 100 μM oroxylin A. Alamar blue assays (Invitrogen) were then performed according to the manufacturer’s ...
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Withaferin-A suppress AKT induced tumor growth in colorectal cancer cells
... study, HCT-116 cells overexpressing AKT showed increased expression of β-catenin, Snail and Slug as detected by Western blot analysis; however, WA treatment led to the downregulation of mesenchymal markers ...
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Oncolytic tanapoxvirus expressing FliC causes regression of human colorectal cancer xenografts in nude mice
... were HCT 116, COLO205, SW1463 and WiDr. Although HCT 116 pro- duced fewer progeny virions than the control cell line OMK, HCT 116 was by far the most productive of the hCRC cell ...
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