HCT-116 cells
Phytochemical Analysis and Anticancer Activity of Seaweed Gracilaria Verrucosa Against Colorectal HCT-116 Cells
... In this research, we use 1000 g dry powder of macroalgae Gracilaria verrucosa, which was macerated in ethyl acetate. Concentrated crude extract of ethylacetate of Gracillaria verrucosa was then fractioned by column ...
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Synthesis and Anticancer Effect of 3,4,5-N-Alkyl-Benzamides on Colon Carcinoma HCT- 116 Cells
... The natural phenolic gallic acid has demonstrated a significant inhibition of cell proliferation in a series of cancer cell lines, as well as induced apoptosis in HCT-116 colon cancer cells. This ...
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... the HCT-116 cells by Lovastatin ...into HCT-116 cells after treatment with Lovastatin has been explained due to decrease in P-gp expression ...
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Evaluation of the Potential Anticancer Activity of Zamzam Water in Human Colon Cancer Cell Line
... of HCT-116 cells with both ZW treatments resulted in morphological changes in HCT-116 cells and inhibited the growth and proliferation of cancer ...in HCT-116 ...
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Induction of cytotoxicity of Pelagia noctiluca venom causes reactive oxygen species generation, lipid peroxydation induction and DNA damage in human colon cancer cells
... The cells were seeded in 6-well plates at 6 × 10 5 ...in HCT 116 cells for 24 h, followed by 1 mM H2O2 for 2 ...The cells were then washed with cold PBS, scraped and lysed by ...
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In vitro and in vivo anti colon cancer effects of Garcinia mangostana xanthones extract
... Briefly, HCT 116 cells were transfected by reverse transfection with DNA constructs of 10 signalling pathways, a positive control, and a negative ...incubation, cells were treated for 6 h in ...
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Omega-3 fatty acid DHA modulates p53, survivin, and microRNA-16-1 expression in KRAS-mutant colorectal cancer stem-like cells
... Briefly HCT-116 cells (5 × 10 5 cells) per well were cultured in the absence or presence of DHA (100, 150, and 200 μM/L) for 48 ...the cells were washed twice with ice-cold phosphate ...
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A Functional Variant of IC53 Correlates with the Late Onset of Colorectal Cancer
... cultured HCT- 116 cells either stably transfected with plasmids carrying IC53 or empty plas- mids, and mRNA was isolated from the total RNA (200 μg) by using the Oligotex mRNA Midi Kit (Qiagen, ...
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Efficacy of 5 FU or Oxaliplatin Monotherapy over Combination Therapy in Colorectal Cancer
... tumor cells via three potential mechanisms: inhibition of thymidylate synthase by FdUMP; incorporation of FdUTP into DNA; and incorporation of FUTP into RNA ...in HCT-116 ...stem cells could ...
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In vitro evaluation of anti-infective activity of a Lactobacillus plantarum strain against Salmonella enterica serovar Enteritidis
... epithelial cells via fimbriae or pili, present on the bacterial cell surface ...epithelial cells. In the present work, colon epithelial cell line, HCT-116 was used to study the adherence and ...
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Overexpression of PKM2 promotes mitochondrial fusion through attenuated p53 stability
... and HCT-116 cell lines by qPCR ...GFP cells, Drp1 expression level was significantly reduced by approximately ...and HCT-116 cells, respectively, but no significant changes in ...
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Inhibition of 6-phosphofructo-2-kinase (PFKFB3) induces autophagy as a survival mechanism
... to cells transfected with a control siRNA (Figure 1A, ...(viable cells [× 10 4 /mL]: 24 hours, control, 89 ± 4 and PFKFB3 siRNA, ...with cells transfected with the siRNA species - 2DG uptake was ...
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SK3/TRPC1/Orai1 complex regulates SOCE-dependent colon cancer cell migration: a novel opportunity to modulate anti- EGFR mAb action by the alkyl-lipid Ohmline
... Figure 6: Ohmline as a new personalized treatment strategy to decrease P-Akt and therefore modulate the effects of Anti-EGFR mAbs. A. Disrupting lipid-rafts with the alkyl-lipid Ohmline allows SK3 to re-translocate ...
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LncRNA CASC9 interacts with CPSF3 to regulate TGF-β signaling in colorectal cancer
... To elucidate the mechanism by which CASC9 regulates CRC cell growth, we attempted to identify its endogenous interaction partners. By analyzing lncRNA – protein inter- actions using CASC9 (Ensembl ID: ENSG00000249395) in ...
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A-kinase anchor protein 4 (AKAP4) a promising therapeutic target of colorectal cancer
... and HCT 116 cells were transfected with AKAP4 shRNA3or NC ...shRNA. Cells were seeded onto 12 mm coverslip and were fixed with ...cancer cells were processed by washing with sodium ...
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Oroxylin A modulates mitochondrial function and apoptosis in human colon cancer cells by inducing mitochondrial translocation of wild-type p53
... Oroxylin A is a flavonoid extracted from the root of Scutellaria baicalensis Georgi. We previously demonstrated that oroxylin A induced apoptosis in human colon cancer cells via the mitochondrial pathway. In the ...
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Withaferin-A suppress AKT induced tumor growth in colorectal cancer cells
... CRC cells, we measured cell proliferation for cells expressing pCMV/HCT-116 or AKT/HCT-116 using MTT, trypan blue and BrdU ...of HCT- 116 transfectants (pCMV and ...
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“Cytotoxicity Study of Pressurized Liquid Extraction of Lignosus rhinocerus (Tiger Milk Mushroom) towards Human Colorectal Cancer Cells (HCT 116)” by C.F. Suziana Zaila, M.Y. Farida Zuraina, M.N. Norfazlina, L. Lek Mun, P. Kok Lun, N. Nurshahirah, L. Florinsiah, A. R. Rumiza, N.F. Rajab, Malaysia.
... Apoptosis is a type of programmed cell death genrally characterized as by distinct morphological characteristic such as cell shrinkage, pyknosis, membrane blebbing etc 17 . It is belived that the cytotoxic action of many ...
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Phytochemistry profile and in vitro cytotoxicity of seaweed macroalgae Sargassum polycystum against colon HCT-116 and lung A-549 cancer cells
... colon HCT-116 cells with IC 50 value of ...A-549 cells has shown by Hex extract of ...colon HCT-116 is ...A-549 cells is ...colon HCT-116 and lung A-549 ...
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lncRNA SNHG6 regulates EZH2 expression by sponging miR-26a/b and miR-214 in colorectal cancer
... CRC cells, be- cause the functions of a lncRNA depended on its subcel- lular distribution ...in HCT-116 ...in HCT-116 cells indicated that only overexpression of miR-214-3p, ...
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