Heat shock protein 1
Heat Shock Protein 90 and Heat Shock Protein 70 Are Components of Dengue Virus Receptor Complex in Human Cells
... containing 1% Triton X-100 and protease inhibitor cocktail (2 mM EDTA, ...for 1 h on ...E protein stimulation, U937 cells were stimu- lated with 500 g of recombinant E protein for 2 h at ...
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Heat shock protein 90 in neurodegenerative diseases
... kinase 1 (PINK1), which encodes a puta- tive mitochondrial serine/threonine kinase, leads to PARK6, an autosomal recessive form of familial Parkin- son's disease ...unstructured protein that may form ...
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Nitric oxide induces heat shock protein 70 expression in vascular smooth muscle cells via activation of heat shock factor 1
... hsp70 protein accumulation in cultured Hep G2 and hepatocytes treated with NO ...to heat shock or stress remains to be ...regulating heat shock response in which the hsps them- selves ...
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Transgenic mice expressing the human heat shock protein 70 have improved post ischemic myocardial recovery
... inducible HSP70 in transgenic mouse hearts. Overexpression of the human HSP70 does not appear to affect normal protein synthesis or the stress response in transgenic mice compared with nontransgenic mice. After 30 ...
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De novo and rare mutations in the HSPA1L heat shock gene associated with inflammatory bowel disease
... Glow-Fold Protein Refolding Kits (K-290, Boston Biochem, Cambridge, MA, USA) according to the manu- facturer’s protocol with ...HSPA1L protein (4 μM), a 1:1 mixture of recombinant HSPA1L WT ...
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Koritala, Bala Subrahmanya Chakravarthy (2016): Circadian entrainment and molecular mechanisms of protein aggregation. Dissertation, LMU München: Medizinische Fakultät
... and protein of hsp-16s was detected using Northern blots and fluorescent imaging, but only is worms exposed to stressors, mainly heat ...(without heat shock exposure or other stress ...
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Enhanced expression of heat shock protein 70 (hsp70) and heat shock factor 1 (HSF1) activation in rheumatoid arthritis synovial tissue Differential regulation of hsp70 expression and hsf1 activation in synovial fibroblasts by proinflammatory cytokines, shear stress, and antiinflammatory drugs
... Nuclear protein extracts were prepared from untreated (C), TNF- a (10 ng/ml), IL-1 (10 ng/ml), IFN- g (30 ng/ml), and indomethacin (IM, 30 mM) treated, as well as shear-stressed (SS) and ...
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Mitochondrial Heat Shock Protein Machinery Hsp70/Hsp40 Is Indispensable for Proper Mitochondrial DNA Maintenance and Replication
... day 1, with the recovery phenotype emerging on day 6 after RNAi induction ...mtHsp40 protein (either wild type or protein C epitope-tobacco etch virus- protein A epitope [PTP] tagged) showed a ...
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Proteomic analysis of acidic chaperones, and stress proteins in extreme halophile Halobacterium NRC-1: a comparative proteomic approach to study heat shock response
... DNA replication, transcription, translation and repair The survival of Halobacterium NRC-1 under extreme envi- ronmental conditions always depends upon the success- ful repair and replication of genetic material. ...
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Heat shock protein and heat shock factor 1 expression and localization in vaccinia virus infected human monocyte derived macrophages
... "optimal" heat shock element (HSE) containing five perfect inverted nGAAn repeats from the human hsp70 ...of protein (5 µ g) in 10% glyc- erol were incubated at room temperature for 30 ...
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Pho dynamically interacts with Spt5 to facilitate transcriptional switches at the hsp70 locus
... file 1: Figure ...Upon heat shock, HSF, along with P-TEFb, is recruited to chromatin and releases RNA polymerase II from the paused ...the heat shock stimulus, the locus should ...
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The L-Type Cyclin CYL-1 and the Heat-Shock-Factor HSF-1 Are Required for Heat-Shock-Induced Protein Expression in Caenorhabditis elegans
... after heat shock, syIs38 animals become very hyperac- tive, moving with such extremely deep flexions that they curl up on ...after heat-shock treatment. However, the effect of ...
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Prolonged morphine administration alters protein expression in the rat myocardium
... Protein spots showing altered abundance levels were excised from the gel, in-gel digested with trypsin, and analyzed by MALDI-TOF MS/MS as described pre- viously [16]. Peptide mass-fingerprinting and subsequent ...
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A gene-specific non-enhancer sequence is critical for expression from the promoter of the small heat shock protein gene αB-crystallin
... Figure 5 Cryab promoter without GPS ( Δ GPS) is inactive in transgenic mice. (A) Schematic of the + GPS cryab promoter-tGFP transgene (GPS, blue rectangle). Primer locations for genotyping are shown. (B) Genotyping of ...
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Identification of epipolythiodioxopiperazines HDN-1 and chaetocin as novel inhibitor of heat shock protein 90
... Three-dimensional structure building and all modeling were performed using the SYBYL-X 1.10 (Tripos Inc., St. Louis, MO, USA) Program Package [39] installed on a FOUNDER Wenxiang E520 workstation running the Red Hat ...
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Response of circulating heat shock protein 70 and anti heat shock protein 70 antibodies to catheter ablation of atrial fibrillation
... Heat shock proteins (HSPs) are characterized as molecu- lar chaperones and have important functions in the pres- ervation and protection of cells and organs from stress and ...
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Proteomic analysis of human periodontal ligament cells under hypoxia
... beta 1 (HSPB1) and heat shock 70 kDa protein 5 (HSPA5), synergistically exerting anti-apoptotic effects on ...ubiquitin-associated protein 1 (UBAP1), and ubiquitin-like ...
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Proteomic analysis of retinal pigment epithelium cells after exposure to UVA radiation
... (PRDX1), heat-shock protein 27 (Hsp27), protein disulfide-isomerase (PDI), calreticulin (CALR), 78 kDa glucose-regulated protein (GRP78), alpha crystalline B chain, cell division ...
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Heat shock factor 1 protein in heat shock factor 1 gene transfected human epidermoid A431 cells requires phosphorylation before inducing heat shock protein 70 production
... HSF1 protein, but not HSP-72; ( b) treatment with the PKC activator PMA resulted in a signifi- cant translocation of HSF1 from the cytosol to the nucleus; ( c) PMA induced HSP-72 production without altering HSF1 ...
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Regulation and Dynamic Behavior of the Heat Shock Transcription Factor Hsf-1 in C. Elegans
... Most of the evidence for human-specific granules comes from the failure to detect them in mouse or hamster cell lines (Denegri et al., 2002; Sarge et al., 1993). This is not due to divergence of the HSF1 molecule: mouse ...
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