Heat-stable enterotoxin
Enteroaggregative Escherichia coli elaborate a heat stable enterotoxin demonstrable in an in vitro rabbit intestinal model
... by heat-stable enterotoxin variant STa ELISA, were negative in the suckling mouse assay, and failed to hybridize with heat-stable enterotoxin variant STh and STp DNA ...partially ...
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Activation of Guanylate Cyclase-C With Heat-Stable Enterotoxin Fails to Potentiate Celecoxib-Induced Reduction in Colorectal Cancer Cell Growth in Vitro
... This study evaluated the use of heat-stable enterotoxin ST, a guanylate cyclase receptor type C GC-C agonist, in combination with celecoxib, a cyclooxygenase-2 COX-2 inhibitor, for the p[r] ...
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Construction of Hybrid Gene of Hepatitis B Surface Antigen Carrying Heat-Stable Enterotoxin of Escherichia coli and Its Expression in Mammalian Cell Line
... Mucosal immunity is difficult to achieve with subunit vaccines unless such vaccines are administered with an adjuvant. Bacterial toxins have been used as either adjuvant or in chimeric forms to incite and sustain an ...
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Blinded, two laboratory comparative analysis of Escherichia coli heat stable enterotoxin production by using monoclonal antibody enzyme linked immunosorbent assay, radioimmunoassay, suckling mouse assay, and gene probes
... Heat-stable enterotoxin ST-producing enterotoxigenic Escherichia coli ETEC can be identified by a variety of assays, including the suckling mouse assay SMA, radioimmunoassay RIA, polyclo[r] ...
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Enzyme linked immunosorbent assay for Escherichia coli heat stable enterotoxin type II
... The gene for Escherichia coli heat-stable enterotoxin type Il ST11 was fused to the genes for protein A from Staphylococcus aureus and Pi-galactosidase in two different expression system[r] ...
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Detection and sequences of the enteroaggregative Escherichia coli heat stable enterotoxin 1 gene in enterotoxigenic E coli strains isolated from piglets and calves with diarrhea
... Some strains of enteroaggregative E. coli (EAggEC), the most recently recognized category of human diarrheagenic E. coli, elaborate an enterotoxin of 38 amino acids named EAggEC heat-stable ...
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Genome and Functional Characterization of Colonization Factor Antigen I- and CS6-Encoding Heat-Stable Enterotoxin-Only Enterotoxigenic Escherichia coli Reveals Lineage and Geographic Variation
... the heat-stable enterotoxin (ST) are more often associated with childhood diarrhea than ETEC strains that encode only the heat-labile enterotoxin ...
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Regulation of taurine transport by Escherichia coli heat stable enterotoxin and guanylin in human intestinal cell lines
... The human colon carcinoma cell lines Caco-2 and HT-29 take up taurine actively. Treatment of Caco-2 cells with Escherichia coli heat-stable enterotoxin (STa) or with guanylin inhibited taurine uptake ...
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High level production of Escherichia coli STb heat stable enterotoxin and quantification by a direct enzyme linked immunosorbent assay
... A convenient and sensitive enzyme-linked immunosorbent assay ELISA for the STb heat-stable enterotoxin of Escherichia coli was developed and used to quantify STb production by strains wi[r] ...
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Disruption of the guanylyl cyclase C gene leads to a paradoxical phenotype of viable but heat stable enterotoxin resistant mice
... of the family include receptors for egg and natriuretic pep- tides, and a subfamily of orphan receptors expressed in sen- sory tissues (1–5). GC-C has been suggested as the receptor for Escherichia coli ...
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Bactericidal effect of selected antidiarrhoeal medicinal plants on intracellular heat-stable enterotoxin-producing Escherichia coli
... Diarrhoeal diseases due to enterotoxigenic Escherichia coli continue to be a cause of global concern. Medicinal plants have been gaining popularity as promising antidiarrhoeal agents. In the present study, four ...
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Production of Escherichia coli STa like heat stable enterotoxin by Citrobacter freundii isolated from humans
... freundii strains, all isolated from children with diarrhea, elaborated an enterotoxin detected by the suckling mouse assay.. A crude extract was prepared by acetone precipitation and a s[r] ...
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Monoclonal antibodies specific for the Escherichia coli heat stable enterotoxin STb
... Protein A-STb and STb-alkaline phosphatase protein fusions were used as immunogen and antigen, respectively, for the generation and screening of monoclonal antibodies to the Escherichia [r] ...
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Detection of Escherichia coli heat stable enterotoxin genes in pig stool specimens by an immobilized, colorimetric, nested polymerase chain reaction
... A combination of selective enrichment by using immunomagnetic separation of F4 K88-positive Escherichia coli and a nested colorimetric polymerase chain reaction PCR was used on crude cli[r] ...
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Trivalent heat labile and heat stable enterotoxin probe conjugated with horseradish peroxidase for detection of enterotoxigenic Escherichia coli by hybridization
... The HRP-conjugated trivalent probe was applied to the detection of enterotoxigenic Escherichia coli ETEC by colony and stool hybridizations.. The binding of the probe to its targets was [r] ...
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Mechanism of action of Escherichia coli heat stable enterotoxin in a human colonic cell line
... the enterotoxin binds to its receptor on the apical membrane. The enterotoxin, by increasing cGMP, opens a K+ channel on the basolateral membrane as well as a Cl- channel on the apical ...
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Factors affecting detection of Yersinia enterocolitica heat stable enterotoxin by the infant mouse test
... Enterotoxigenicity and the temperature at which mice were kept were interrelated, such that for weakly enterotoxigenic strains there were no significant differences among gut/carcass wei[r] ...
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Simplification of methods for the production and storage of specimens to be tested for heat stable enterotoxin of Escherichia coli
... Experiments with the infant mouse test demonstrated that there is no need of shaking for heat-stable Escherichia coli enterotoxin production when low volume of medium per volume of flask[r] ...
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Enzyme linked immunosorbent assay for Escherichia coli heat stable enterotoxin
... The sensitivity of an enzyme-linked immunosorbent assay ELISA to detect pure native Escherichia coli heat-stable toxin ST and to identify ST-producing strains among clinical isolates was[r] ...
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Simultaneous detection of Escherichia coli heat stable and heat labile enterotoxin genes with a single RNA probe
... A single RNA probe was synthesized and used to detect simultaneously the methanol-soluble heat-stable enterotoxin and heat-labile enterotoxin genes in Escherichia coli strains.. The resu[r] ...
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