Heat Stable Enterotoxin (ST)
Genome and Functional Characterization of Colonization Factor Antigen I- and CS6-Encoding Heat-Stable Enterotoxin-Only Enterotoxigenic Escherichia coli Reveals Lineage and Geographic Variation
... the heat-stable enterotoxin (ST) are more often associated with childhood diarrhea than ETEC strains that encode only the heat-labile enterotoxin ...
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Blinded, two laboratory comparative analysis of Escherichia coli heat stable enterotoxin production by using monoclonal antibody enzyme linked immunosorbent assay, radioimmunoassay, suckling mouse assay, and gene probes
... Heat-stable enterotoxin (ST)-producing enterotoxigenic Escherichia coli (ETEC) can be identified by a variety of assays, including the suckling mouse assay (SMA), radioimmunoassay (RIA),[r] ...
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Enzyme linked immunosorbent assay for Escherichia coli heat stable enterotoxin type II
... The gene for Escherichia coli heat-stable enterotoxin type Il (ST11) was fused to the genes for protein A from Staphylococcus aureus and Pi-galactosidase in two different expression syst[r] ...
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Mechanism of action of Escherichia coli heat stable enterotoxin in a human colonic cell line
... coli heat stable enterotoxin (STa) caused Cl- secretion across T84 cell monolayers in a dose-dependent manner only when applied to the apical membrane surface and not when applied to the basolateral ...
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Enteroaggregative Escherichia coli elaborate a heat stable enterotoxin demonstrable in an in vitro rabbit intestinal model
... by heat-stable enterotoxin variant STa ELISA, were negative in the suckling mouse assay, and failed to hybridize with heat-stable enterotoxin variant STh and STp DNA ...partially ...
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Regulation of taurine transport by Escherichia coli heat stable enterotoxin and guanylin in human intestinal cell lines
... J Clin Invest. 1995;96(1):361-369. https://doi.org/10.1172/JCI118042. The human colon carcinoma cell lines Caco-2 and HT-29 take up taurine actively. Treatment of Caco-2 cells with Escherichia coli ...
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High level production of Escherichia coli STb heat stable enterotoxin and quantification by a direct enzyme linked immunosorbent assay
... A convenient and sensitive enzyme-linked immunosorbent assay (ELISA) for the STb heat-stable enterotoxin of Escherichia coli was developed and used to quantify STb production by strains [r] ...
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Activation of Guanylate Cyclase-C With Heat-Stable Enterotoxin Fails to Potentiate Celecoxib-Induced Reduction in Colorectal Cancer Cell Growth in Vitro
... Activation of Guanylate Cyclase-C With Heat-Stable Enterotoxin Fails to Potentiate Celecoxib-Induced Reduction in Colorectal Cancer Cell Growth in Vitro.. The Harvard community has made [r] ...
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Construction of Hybrid Gene of Hepatitis B Surface Antigen Carrying Heat-Stable Enterotoxin of Escherichia coli and Its Expression in Mammalian Cell Line
... of heat-stable enterotoxin of Escherichia coli that is capable of adhering to epithelial cells was inserted at amino acid position 112 of hepatitis surface ...
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Detection and sequences of the enteroaggregative Escherichia coli heat stable enterotoxin 1 gene in enterotoxigenic E coli strains isolated from piglets and calves with diarrhea
... 36. Wray, C., I. M. Mclaren, and P. J. Carroll. 1993. Escherichia coli isolated from farm animals in England and Wales between 1986 and 1991. Vet. Rec. 133:439–442. 37. Yamamoto, T., and P. Echeverria. 1996. Detection of ...
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Enzyme linked immunosorbent assay for Escherichia coli heat stable enterotoxin
... The ELISAs in which crude antiserum to the ST(S) prepara- tion, which has the same antigenicity as pure native ST, was used were less sensitive than the suckling mouse assay and were una[r] ...
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Simplification of methods for the production and storage of specimens to be tested for heat stable enterotoxin of Escherichia coli
... Consequently, to achieve this aim the following factors were studied as far as their effect on ST production was concerned: (i) whether shaking of colibacilli cultures is necessary for S[r] ...
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Monoclonal antibodies specific for the Escherichia coli heat stable enterotoxin STb
... Protein A-STb and STb-alkaline phosphatase protein fusions were used as immunogen and antigen, respectively, for the generation and screening of monoclonal antibodies to the Escherichia [r] ...
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Factors affecting detection of Yersinia enterocolitica heat stable enterotoxin by the infant mouse test
... Since higher ambient temperatures have a more pronounced effect on the assay of weaker strains, the results will vary depending on the strains under examination and the ambient temperatu[r] ...
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Trivalent heat labile and heat stable enterotoxin probe conjugated with horseradish peroxidase for detection of enterotoxigenic Escherichia coli by hybridization
... A total of 107 to 101 cells of ETEC and non-ETEC strains were incubated at 37°C overnight and used as targets for hybridization with the HRP-conjugated LTh-STIa-STIa-STIb probe. Hybridiz[r] ...
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Production of Escherichia coli STa like heat stable enterotoxin by Citrobacter freundii isolated from humans
... evaluation of the Biken test (modified Elek test) for detection of enterotoxigenic Escherichia coli producing heat-labile enterotoxin and application of the test to sampling of heat-stab[r] ...
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Bactericidal effect of selected antidiarrhoeal medicinal plants on intracellular heat-stable enterotoxin-producing Escherichia coli
... The Foundation for Medical Research, 84A, RG Thadani Marg, Worli, Mumbai-400018, India Birdi, et al.: Immunomodulatory Activity of Anti-Diarrhoeal Plants Diarrhoeal diseases due to enterotoxigenic Escherichia coli ...
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Disruption of the guanylyl cyclase C gene leads to a paradoxical phenotype of viable but heat stable enterotoxin resistant mice
... Suckling mouse assay. To assess the intestinal secretory response to E. coli STa, 3-d-old pups were given an oral inocu- lum of 10 U of STa. A unit of STa is defined as that amount of enterotoxin that yields a ...
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Real Time Fluorescence PCR Assays for Detection and Characterization of Heat Labile I and Heat Stable I Enterotoxin Genes from Enterotoxigenic Escherichia coli
... the enterotoxin genes commonly present in strains associated with human ...I heat-labile enterotoxin (LT I) and the type I heat-stable enterotoxin (ST I) genes, using the LC ...
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Detection of Escherichia coli heat stable enterotoxin genes in pig stool specimens by an immobilized, colorimetric, nested polymerase chain reaction
... A combination of selective enrichment by using immunomagnetic separation of F4 (K88)-positive Escherichia coli and a nested colorimetric polymerase chain reaction (PCR) was used on crude[r] ...
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