HEK293/T
The cytosolic N-terminus of CD317/tetherin is a membrane microdomain exclusion motif.
... (E) HEK293-T cells were transfected with the indicated CD317-HA-SR constructs and, 24 hours later, incubated with an anti-HA antibody before surface expression was monitored by flow cytometry in the PE ...
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Foot-and-Mouth Disease (FMD) Virus 3C Protease Mutant L127P: Implications for FMD Vaccine Development
... two-tailed t test (P ⬍ 0.001). (C) Western blots of transfected HEK293-T cell lysates showing P1 products from five FMDV serotypes processed by ...
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Attenuation of nicotine evoked Ca2+ influx by antibody to the nicotinic acetylcholine receptor α3 subunits in human embryonic kidney cells
... mock-transfected HEK293 cells were fixed in ice-cold 4% paraformaldehyde (PFA) for 20 min, washed in phosphate-buffered saline (PBS), blocked in PBS containing 1% BSA and ...
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Human and Rhesus APOBEC3D, APOBEC3F, APOBEC3G, and APOBEC3H Demonstrate a Conserved Capacity To Restrict Vif-Deficient HIV-1
... pear remarkably similar in organization and function. The genomic loci are syntenic, each composed of seven genes ar- ranged in tandem to encode seven proteins, three with one zinc-coordinating domain and four with two ...
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Fusarochromanone-induced reactive oxygen species results in activation of JNK cascade and cell death by inhibiting protein phosphatases 2A and 5
... in HEK293 and COS7 cells, which is, to some extent, different from the findings in other mycotoxins ...to T-2 toxin induces ROS and the phosphorylation of Erk1/2, p38 MAPK and JNK, causing apoptosis in ...
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Spatial localisation of Discoidin Domain Receptor 2 (DDR2) signalling is dependent on its collagen binding and kinase activity.
... Flp-In T-Rex HEK293 cell line which showed a slight decrease in total receptor expression levels compared to the wild- type DDR2-expressing cells ...
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Adeno-Associated Virus Production in Serum-Free Suspension Adapted HEK293 Cells for Gene Therapy.
... the T-25 flasks were trypisnized and counted on a Vicell to determine the amounts of plasmids required for ...The T-25 flasks were removed from the incubator and the spent media was aspirated out and ...
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Association of CAD, a multifunctional protein involved in pyrimidine synthesis, with mLST8, a component of the mTOR complexes
... from HEK293 cells transfected with the FLAG-mLST8 vector or an empty ...CAD, T-complex protein α and ε, T-complex protein η and θ, and tubulin β5, respectively (data not ...from HEK293 cells ...
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The small molecule NSC676914A is cytotoxic and differentially affects NFκB signaling in ovarian cancer cells and HEK293 cells
... Ovarian cancer cell line OVCAR3 was a gift from Elise Kohn (NCI, Bethesda, MD), HEYA8 was a gift from Gordon Mills (MD Anderson Cancer Center, Houston, TX), and 1A9 cells were a gift from A.T. Fojo (NCI, Bethesda, MD); ...
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Echinacea induced cytosolic Ca2+ elevation in HEK293
... In T-cells, for example, elevated intracellu- lar Ca 2+ activate Ca 2+ -dependent enzymes, such as calci- neurin, and thereby transcription factors, such as nuclear factor of activated T cells (NFAT) and ...
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The Development of Peptide Analogues as Potential Fluorescent and PET Imaging Probes.
... using HEK293 cells transfected with GHS-R1a (obtained from ...source. HEK293 cells were grown in MEM (Minimum Essential Media) supplemented with non-essential amino acids and 10% fetal bovine ...and ...
238
DNA Damage Signaling Is Required for Replication of Human Bocavirus 1 DNA in Dividing HEK293 Cells
... human T lymphotropic virus type 1 (HTLV-1) can form pseudo-DDR foci in cells by tethering MDC1 to chromatin and recruiting DDR factors, including ␥ H2AX and activated DNA- PKcs (63, ...
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Expression of glycosylated human prolactin in HEK293 cells and related N-glycan composition analysis
... Prolactin (PRL) is a hormone produced by the pituitary gland with innumerable functions, such as lactation, repro- duction, osmotic and immune regulation. The present work describes the synthesis of hPRL in human ...
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The calcium-sensing receptor regulates parathyroid hormone gene expression in transfected HEK293 cells
... In vivo dietary-induced hypocalcemia and hypophos- phatemia as well as adenine-induced renal failure and the calcimimetic R568 lead to post-translational modifica- tions of AUF1 as shown by 2D gels [7,27]. To determine ...
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Sodium Nitroprusside inhibits HEK293 Cell Growth by cGMP Dependent and Independent Mechanisms
... Exogenously Added cGMP, Cell Growth, and Levels of cGMP, GSH and GSSG In order to determine the ability to mimic the SNP effect on cell growth cGMP was added to the culture medium in con[r] ...
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Microtubule-Mediated and Microtubule-Independent Transport of Adenovirus Type 5 in HEK293 Cells
... in HEK293 cells, which are routinely used in this laboratory because they are permissive for replication of multiple adenovirus serotypes, a different trafficking pattern has been ...
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Fabrication and evaluation of multiple pDNA-hydroxyapatite particles for transfection to HEK293 cells
... for HEK293 cells transfected with three different single marker-loaded pDNA-HA particles, co-administration of these pDNA-HA particles, ...RFP-positive HEK293 cells were seen after transfection with pDNA-HA ...
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Microcystin-LR (MCLR) Induces a Compensation of PP2A Activity Mediated by α4 Protein in HEK293 Cells
... Figure 2. PP2A shows opposing activity with diffuse alternations of its core enzyme protein level and post-translational modification under MCLR treatment. HEK293 cells were exposed to varying concentrations of ...
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Carcinogenic activity of PbS quantum dots screened using exosomal biomarkers secreted from HEK293 cells
... cells to PbS QDs have not been evaluated comprehensively. In this study, PbS QDs modified by 3-mercaptopropionic acid (MPA) were synthesized, and their toxic effects on HEK293 cells were evaluated by utilizing an ...
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Carboplatin Enhances the Activity of Human Transient Receptor Potential Ankyrin 1 through the Cyclic AMP-Protein Kinase A-A-Kinase Anchoring Protein (AKAP) Pathways
... Transient receptor potential ankyrin 1 (TRPA1) is a polymodal nociceptor (pain sensor) expressed in primary sensory neurons (DRG neurons) and involved in pain transmission [10-14]. TRPA1 is activated by noxious stimulus, ...
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