The quality control of herbal drugs and their extracts is essential when they are used as raw material for the development of herbal medicines. In this work, several techniques (pharmacopoeial and non-pharmacopoeial) were performed, aiming at establishing quality control parameters of the leaves of Heliotropium indicum L. (Boraginaceae), a shrub popularly known as stinker (fedegoso) in Brazilian northern and northeastern regions. The results determined the physicochemical characteristics of the drug and the tincture of the leaves. They also showed that the values obtained are in accordance with the data found in the literature for plant material. Preliminary phytochemical analysis of the leaves demonstrated the presence of phenols, tannins and alkaloids. The chromatographic profile showed the presence of a common substance in the hexanic and alkaloidal fractions.
The experiment examined the potential of some organic extracts to control African army worm on maize plantations. The experiment was laid out in a randomized complete block design (RCBD) consisting of four treatments each replicated four times. Treatments consisted of extracts from the leaves of Heliotropium indicum (HE), extract from dried chilli pepper (CPE), combination of H.indicum with dried chilli pepper extract (HE + CPE) and Cypermethrine (CP) insecticide incorporated in the study for comparison with the botanicals and the untreated check as control (CL). A susceptible and improved maize variety SAMARU 38 was used for the experiment. Data were collected on the following parameters for each treatment: Number of plants infested before and after treatment, percentage damage before and after treatment, total damage after treatment, percentage level of infestation after treatment, and percentage yield increase. All treatments at different plant growth stages reduced the number of holes on the maize plant and these values were significantly different from that of the untreated control plot. Field results indicated that extract of H. indicum, chili pepper and their combinations were effective in controlling army worm. Grain yield in extracts of H.indicum were higher than monocrotophos treatments and the untreated check. Incorporating the plant extracts treatments into sustainable maize production will help to improve the crop yield in resource-poor-farmers field, as well as increase monetary returns for their investment without altering the eco system.
The aim of the present study was to investigate phyto chemical screening and anti-oxidant activity of the ethanol extract of the whole plant of Heliotropium indicum. The ethanolic extract of the whole plant of Heliotropium indicum was studied for its anti-oxidant effect. During phytochemical screening flavonoids and phenols were found abundantly. Ethanolic extract of Heliotropium indicum was screened to evaluate free radical scavenging effect. The extract exhibited significant reducing power and free radical scavenging effect. This antioxidant activity was concentration dependant which was compared with standard antioxidant such as Ascorbic acid.
Heliotropium indicum. Linn. Belongs to family Boraginaceae. This family is well marked in their characteristic and not easily confused with any other. A majority of the plant in family are herbs. The genus Heliotropium comprises about 250 species and is distributed in tropical, subtropical and warm temperature zones of all continents but only a few species have been systematically investigated.  The plant is annual, erect, branched hirsute plant about 15 to 50cm high. The leaves are always opposite or alternate, ovate to oblong- ovate, somewhat hairy, acute or acuminate, base decurrently along the petiole and about 3 to 8cm long. The flowers are calyx green and about 3.5mm in diameter. The fruits are dry 2 to 4 lobed of 2 or 4 nearly free, more or less united nutlets, 4 to 5mm long. 
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ABSTRACT: Heliotropium indicum Linn., commonly known as ‘Indian heliotrope’ is very common in India with a long history of traditional medicinal uses in many countries in the world. The plant is reported to possess antibacterial, antitumor, uterine stimulant effect, antifertility, wound healing, anti-inflammatory, antinociceptive and diuretic activities. Several pyrrolizidine alkaloids have been isolated from this species. The active principle Indicine-N-oxide has reached Phase 1 clinical trials in advanced cancer patients. But severe toxic side-effects showed that a therapy with indicine-N-oxide was not justiﬁed. Most of the alkaloids are hepatoxic and therefore internal use of Heliotropium species is not recommended. External application to promote wound healing and to fight infections seems to be less hazardous, but more research is needed. The comprehensive account of the chemical constituents and the biological activities are presented in this review such that the potential use of this plant in various traditional medicines can be systematically evaluated.
Objective: In Indian sub-continent occurrence of cervical cancer is second among women. Most cervical cancer cases are associated with HPV 16. In this study, the anti-proliferative activity of chloroform fraction of methanolic extract of Heliotropium indicum was studied in a panel of cervical cancer cell lines. Methods: HeLa (HPV 18 positive), SiHa (HPV 16 positive) and C33A (HPV negative) cells were treated with IC 50 doses and experiments were performed to evaluate the cell death process. To chemically characterize the extract fraction, GC-MS, LC-MS and column chromatography were performed. Results: Typical apoptotic features were found in the treated cells. Most cells were found in late apoptotic stages. In HeLa and C33A cell lines, cells were blocked at G1/S, in SiHa, subG0 population increased. Expression of anti-apoptotic Bcl-2 decreased and that of pro-apoptotic BAX, p53, p21 increased. Expression of HPV16 and human ET-1 also decreased. ROS mediated DNA damage was detected. Different fatty acids along with other compounds like phenolics, alkaloids were detected in the chloroform fraction, all of which are reported to have anticancer properties. Conclusion: From the study, it can be concluded that fatty acid rich chloroform fraction of H. indicum is capable of inducing apoptosis in cervical cancer cells, through ROS mediated DNA damage.
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Diversify the endophytic fungi associated from the leaves of Heliotropium indicum it’s a rare reports are find the diversity of endophytic fungi in Helitropium species. Even though much more species of endophytic fungi can be expected from the plant, the conditions and media used in the current study might have favoured the growth of the species obtained. Even these fungi can be a novel source for the production of compounds which can have a diverse implication. Currently we are working on the characterization of the biologically active metabolites from the isolated endophytic fungi.
The nephroprotective effect of whole plant of ethanolic extract of Heliotropium indicum was confirmed by the following measures: In Rifampicin treated group rise in urea, uric acid and creatinine in blood and urine and decrease in the level of protein. The same is observed in kidney diseases in clinical practice and hence are having diagnostic importance in the assessment of kidney function.
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Herbal products derived from plant extracts are more progressively being used to treat a broad range of clinical diseases. Despite the minimal understanding about their mode of action, there is an increasing interest in the pharmacological evaluation of various plants used in the long-established Indian traditional systems of medicine. Thus, we carried out the present investigation to evaluate the anti-nociceptive and anti-inflammatory action of Heliotropium indicum in mice and rats respectively.
These meta bolites have been isolated from a wide variety of plants, especially from genera belonging to the boraginaceae family 9-11 . The genus Heliotropium , a well-known source of such alkaloids 12-14 and other minor compounds , such as flavonoids and geranyl aromatic derivatives , is constituted of about 250 species represented by herbs and shrubs, distributed throughout the India In the a search for biologically active compounds from the EtOH extract from leaves of Heliotropium Indicum L. popularly known as fedegosa ,found abundantly in the region of northeast in India was investigated this species is
(15 mLkg − 1 of 5 % dextrose, i.v.), in New Zealand White rabbits pretreated with Heliotropium indicum aqueous extract (HIE) (30 – 300 mgkg − 1 ), acetazolamide (5 mgkg − 1 ), and normal saline (10 mLkg − 1 ) per os were measured. IOPs were also monitored in chronic glaucoma in rabbits (induced by 1 % prednisolone acetate drops, 12 hourly for 21 days) after treatments with the same doses of HIE, acetazolamide, and normal saline for 2 weeks. The anti-oxidant property of the extract was assessed by assaying for glutathione levels in the aqueous humour. Glutamate concentration in the vitreous humour was also determined using ELISA technique. Histopathological assessment of the ciliary bodies was made. Results: The extract significantly reduced intraocular pressure ( p ≤ 0.05 – 0.001) in acute and chronic glaucoma, preserved glutathione levels and glutamate concentration ( p ≤ 0.01 – 0.001). Histological assessment of the ciliary body showed a decrease in inflammatory infiltration in the extract and acetazolamide-treated group compared with the normal saline-treated group.
concentration should not be exceeded. This work is limited to establishing the histo-gastroprotective potentials of Heliotropium Indicum and could not ascertain the plant’s active ingredients and their respective potency due to available limited laboratory facilities. It is believed that other substances may still be interfering with the active ingredients and that if the active ingredients can be made purer, they will prove to be more potent. Corresponding Author :
been reported to be responsible for pain sensation caused by intraperitoneal administration of acetic acid. [20, 21] The extract produced significant writhing inhibition comparable to the standard drug diclofenac sodium (Table 2). The chemical compounds present in the plant extract may be responsible for the obtained antinociceptive activity. Based on this result it can be concluded that the ethanol extract of Heliotropium indicum Linn. might possess antinociceptive activity.
of H.indicum may be attributed to the phytoconstituents present in it, which may be either due to their individual or additive effect that fastens the process of wound healing. Between the two extracts studied, the ethanol leaf extract was found to possess better wound-healing property. However which components of the extract are responsible for this effect was not investigated. Further phytochemical studies are in progress where the ethanol extract will be subjected to further fractionation and purification to identify and to isolate the active compound(s) responsible for these pharmacological activities. The present findings provide scientific evidence to some of the ethno medicinal properties of H. indicum.
Heliotropium indicum (Boraginaceae) is used in the traditional Ivory Coast pharmacopeia to treat asthma. In the present study, wound healing effect of n- butanol fractions was evaluated in H292- cells. Fractions which possessed better wound healing activity were fractionated on Sephadex LH20 column chromatography. Two compounds have been isolated which were responsible for this wound healing effect. Their structures were established as Pestalamide B (1) and Glycinamide,N-(1-oxooctadecyl)glycyl-L-alanylglycyl-L-histidyl (2) on the basis of spectral analysis. Both compound 1 and 2 presented wound healing effect compared with the control (P<0.05).
friendly antimicrobial that is commonly used against many bacterial and fungal pathogens (Koperuncholan et al 2010). The silver-based inorganic antimicrobial/ anticancer agents were produced in the forms of silver-supported inorganic powders, silver colloids, metal silver powders (Anitha, 2011). The main object of the present study is to synthesize the Ag nanoparticles by using green plants against certain pathogens and cancerous cells. To understand the antimicrobial/ anticancer efficacy of biosynthesized Ag nanoparticles by using Heliotropium indicum plant in the medicinal fields.
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Weight loss measurements indicate that the dissolution rate decreases to significant extent due to the presence of oil especially at high concentrations. The inhibition efficiency is maximum on immersion time 1 hour at 6g/lit Sesmum indicum. The maximum inhibition efficiency for immersion time 24 hours at 6g/lit Sesmum indicum is 65.85%.The graph which is present in figure 1 shows that %IE is varies with the amount of the Sesmum indicum oil with variable experimental times like 1 hour, 3 hours, 6 hours, 15 hours and 24 hours.
It has been unveiled that Abutilon indicum contains many biologically active compounds such as phenols, tannins, alkaloids, flavanoids glycosides, proteins, amino acids, sesquiterpenes, steroids, sterols, terpenoids, terpenes, carbohydrate, β– sitosterol, gallic acid, p-coumaric acid, quercetin-3- O-beta glucopyranoside etc. . So, the anti- microbial effect of the extracts is due to any of these compound(s). However, further analysis is necessary to separate these compounds and to find out the most active compound having antimicrobial effect as well as to clarify the actual mode of microbial growth inhibition of that compound. Acknowledgments
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The animals acclimatized to our laboratory conditions were divided into six groups viz. G1, G2, G3, G4, G5 and G6 of six each and used for the study  . The EAC cells were injected intraperitoneally (10 6 cells/mouse) to all the mice of the six groups. On the second day the animals of G2 and G3 were treated with 500 and 1000 mg/kg, i.p.,  of ethanolic extract of Abutilon indicum (EAI), respectively. G4 and G5 were treated with 500 and 1000 mg/kg, i.p.,  of aqueous extract of Abutilon indicum (AAI), respectively, while the mice of G6 were treated with 20 mg/kg of 5-fluorouracil  and the treatment was continued for the next 10 days. G1 was not allocated any treatment after inoculation with EAC cells. The mice were observed for the next 11 days for the development of carcinoma. On day 11, the following parameters were estimated.
Microscopic characters of powder of both Abutilon seeds The powder characters of both drugs (Fig-14, 15, 16&17) have been almost similar. Main diagnostic characters were the type of trichomes and crystals in the cotyledons, like A. indicum contain stellate type of trichomes only and cotyledon embedded with cluster & rosette crystals (Fig-20&18), where A. glaucum contain stellate & simple trichomes also but devoid of crystals (Fig-21&19).