Helper-dependent adenoviral vector
A New Hybrid System Capable of Efficient Lentiviral Vector Production and Stable Gene Transfer Mediated by a Single Helper-Dependent Adenoviral Vector
... LA vector system, an HDAdV was used because of its large cloning capacity (up to 35 kb) ...LA vector to regulate LV ...the vector and thus reduce leaky expres- sion of the lentiviral proteins (10, ...
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Cre Levels Limit Packaging Signal Excision Efficiency in the Cre/loxP Helper-Dependent Adenoviral Vector System
... HD vector and a helper virus bearing a packaging signal flanked by loxP ...the helper virus genome unpackageable but still able to replicate and provide helper functions for HD vector ...
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Intranasal immunization with a helper dependent adenoviral vector expressing the codon optimized fusion glycoprotein of human respiratory syncytial virus elicits protective immunity in BALB/c mice
... E1-E3-deleted helper virus H14 (Microbix) 16 h after ...with helper virus and crude lysates from the previous ...and helper virus, purified by CsCl ...contaminated helper virus was also ...
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Regulated and Liver-Specific Tamarin Alpha Interferon Gene Delivery by a Helper-Dependent Adenoviral Vector
... To determine the percentage of transduced cells expressing tIFN, B76.1/HuH7 cells were infected with HD-TET-tIFN, and cell DNA was extracted 48 h later. HD-TET-tIFN ge- nomes were quantitated by TaqMan PCR assay (see ...
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Epithelium-specific Ets transcription factor-1 acts as a negative regulator of cyclooxygenase-2 in human rheumatoid arthritis synovial fibroblasts
... control helper‑dependent adenoviral vector; cDNA: complementary deoxyribonucleic acid; C/EBP: CAAT enhancer binding protein; COX‑2: cyclooxygenase‑2; CRE: cyclic AMP response elements; ...
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Toxicity Associated with Repeated Administration of First-Generation Adenovirus Vectors Does Not Occur with a Helper-Dependent Vector
... of adenoviral vec- tors to a single immunocompetent individual due to the development of neutralizing anti- bodies, the use of alternative serotypes (48–50) or transient immunosuppression (41–46) may eventually ...
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Chromosomal Integration of Adenoviral Vector DNA In Vivo
... Ad vector with the E1 deletion (30) into FAH ⫺/⫺ mice, the devel- opment of FAH ⫹ nodules in the livers of the experimental animals was observed, suggesting potential chromosomal integra- tion of vector ...
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Adenoviruses in Gene Therapy A Review
... "helper-dependent" adenoviral vectors Recently, "gutless" adenoviral vectors - vectors that are devoid of all viral-protein-coding DNA sequences-have been developed ...this ...
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Subretinal gene delivery using helper-dependent adenoviral vectors
... HD-Ad vector as a vehicle for gene delivery to human retinal ...8 vector particles (vp)/eye, mice showed robust transgene expression along the retinal layer (Figure ...
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12816.full.pdf
... a vector that lacked E1, E2, E3, and late gene expression suggested that the DNA was not stable in the vector-transduced cells, leading to shorter transgene persistence than with first-generation vectors ...
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Characterization of transgene expression in adenoviral vector based HIV 1 vaccine candidates
... The data suggest that for an experimental adenovirus- based HIV-1 vaccine, transgene expression in non-per- missive cells in vitro can be reproducibly modulated by the presence of additional replication-incompetent aden- ...
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CRM1-Dependent Transport Supports Cytoplasmic Accumulation of Adenoviral Early Transcripts
... of adenoviral early and late proteins contains a leucine-rich nuclear export sig- nal of the HIV-1 Rev type, known to be recognized by the cellular export receptor ...supporting adenoviral replication has ...
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woLiver Regeneration Effect of Oncostatin M Following Hepatectomy for the Rat Cirrhotic Liver Model
... and adenoviral vector expressing OSM (AdOSM) allows a persistent expression of the ...AdOSM, adenoviral vector carrying β-galactosidase (AdLacZ), or phosphate-buffered saline ...the ...
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Detection of Transgenes in Gene Delivery Model Mice by Adenoviral Vector Using ddPCR
... For acute experiments, total DNA was extracted from collected stool, plasma, and blood cell 224.. fractions.[r] ...
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Infectious Clones and Vectors Derived from Adeno-Associated Virus (AAV) Serotypes Other Than AAV Type 2
... single-stranded dependent parvoviruses being developed as transduc- ing ...AAV6 vector serotypes were able to transduce cells in the presence of serum from animals previously exposed to AAV2 ...
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Zhang, Wenli (2012): Adenovirus-based gene therapy approaches for hemophilia B. Dissertation, LMU München: Medizinische Fakultät
... different vector systems for hemophilia B gene therapy Retroviral vectors integrate into the target cell genome, which ensures stable maintenance of the DNA and allows long-term transgene expression, but can also ...
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Persistence of recombinant adenovirus in vivo is not dependent on vector DNA replication.
... episomal vector can persist for more than a year in rodents ...the vector. Elucidation of the mechanism for vector maintenance is critical for designing an optimal vector which can persist ...
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ajrcmb.18.3.2784.pdf
... by adenoviral (Ad) and retroviral (RV) vectors was ...Ad vector with specificity for the Ad fiber knob, and in- ternalize vector ...internalize vector by less efficient ...solely ...
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Germline Transformation of Drosophila virilis Mediated by the Transposable Element hobo
... A laboratory strain of Drosophila uirilis was genetically transformed with a hobo vector carrying the miniwhite cassette using a helper plasmid with an hsp7Odriven hobo[r] ...
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Reversal of impaired wound repair in iNOS deficient mice by topical adenoviral mediated iNOS gene transfer
... Gene transfer using iNOS offers some attractive advan- tages over other therapeutic approaches to promote wound re- pair based on increasing wound NO levels in NO-deficient states. Topical application of the iNOS gene in ...
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