Hepatic stellate cell proliferation
GW501516-activated PPARβ/δ promotes liver fibrosis via p38-JNK MAPK-induced hepatic stellate cell proliferation
... (oval) cell response and decreased fibrosis in mice fed with a choline deficient, methionine supplemented diet, while the PPARβ/δ ligand GW501516 did not affect oval cell proliferation or liver ...
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hUCMSC-extracellular vesicles downregulated hepatic stellate cell activation and reduced liver injury in S. japonicum-infected mice
... and proliferation and decreased its expression of pro-fibrogenic markers α-SMA, collagen 1, and collagen 3 stimulated by TGF-β1 in ...suppress hepatic stellate cell proliferation and ...
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Effect of simvastatin on the transforming growth factor β1, fibroscan scores, and aspartate transaminase to platelet index ratio patients with Liver Cirrhosis
... reduce hepatic venous pressure and increase hepatic perfusion in patients with cirrhosis (Albraldes, ...of hepatic stellate cell of the liver (Shirin et al, 2013), reducing ...
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DDR2 receptor promotes MMP 2–mediated proliferation and invasion by hepatic stellate cells
... generate cell lines expressing mutant DDR2 con- structs, the following cDNAs were subcloned down- stream of the viral LTR: wild-type DDR2 (wt-DDR2), the full-length mouse cDNA sequence with a Myc tag at the ...
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SP1 and UTE1 Decoy ODNs inhibit activation and proliferation of hepatic stellate cells by targeting tissue inhibitors of metalloproteinase 1
... Cells were collected for western blot assay after Decoy ODNs were transfected into HSC-T6 cells for 48 h. Then cells were lysed in lysis buffer (25 mmol/L Tris–HCl pH 7.5, 2.5 mmol/L EDTA, 137 mmol/L NaCl, 2.7 mmol/L ...
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miR 140 3p Knockdown Suppresses Cell Proliferation and Fibrogenesis in Hepatic Stellate Cells via PTEN Mediated AKT/mTOR Signaling
... from Cell Signaling Technology (Dan- vers, MA, USA) and Abcam (Cambridge, UK): α-SMA (#14968), COL1A1 (#84336), PCNA (#13110), cleaved caspase 3 (#9664), caspase 3 (#9665), p-AKT (#2965), p-mTOR (#2971), AKT ...
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Original Article Diisopropylamine dichloroacetate alleviates liver fibrosis through inhibiting activation and proliferation of hepatic stellate cells
... the cell counts and morphological changes were observed (Figure ...cells proliferation was that DPDA may decompose into small mol- ecules, then through a certain way into cells, causing burst of cell ...
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HSCs-derived COMP drives hepatocellular carcinoma progression by activating MEK/ERK and PI3K/AKT signaling pathways
... activated hepatic stellate cell line LX2 and 5 HCC cell lines as well as one immortalized liver cell line LO2 were tested by Western blot ...in cell culture supernatant as ...
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Hepatic stellate cells in liver development, regeneration, and cancer
... stem cell therapies, an understanding of liver regenera- tion has important implications for improving current methods of differentiating and propagating hepatocytes in vitro, as well as for stimulating ...
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Original Article Interleukin-26 promotes the proliferation and activation of hepatic stellate cells to exacerbate liver fibrosis by the TGF-β1/Smad2 signaling pathway
... of hepatic stellate cells (HSCs) and the deposition of extra cellular matrix ...By cell counting kit (CCK)-8 assay, we observed that IL-26 significantly promoted the proliferation of HSCs by ...
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Mechanisms of spontaneous resolution of rat liver fibrosis Hepatic stellate cell apoptosis and reduced hepatic expression of metalloproteinase inhibitors
... We have used highly pure cultures of activated stellate cells to confirm that activated HSCs undergo apoptosis in cell cul- ture. The model of activation by culture on plastic in the pres- ence of serum has ...
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Babao Dan attenuates hepatic fibrosis by inhibiting hepatic stellate cells activation and proliferation via TLR4 signaling pathway
... the figures indicated, BBD can obviously down-regulate the expression of TLR4 in rat livers insulted by DEN (Figure 3E). At the same time, the expression of TLR4 was also analyzed by RT-PCR and Western blot, accordingly ...
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Hepatic stellate cells: central modulators of hepatic carcinogenesis
... HCC cell lines, seen in non-treated conditioned media ...HCC proliferation in Hep3B and MHCC97L cell lines ...of hepatic HGF and its receptor were elevated at the time point at which ...
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COMMD1-deficient dogs accumulate copper in hepatocytes and provide a good model for chronic hepatitis and fibrosis
... to hepatic copper accumulation and chronic hepatitis representing a Wilson’s disease like ...on hepatic stellate cell (HSC) activation marker (alpha-smooth muscle actin, a-SMA), ...
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Proliferation of hepatic stellate cells is inhibited by phosphorylation of CREB on serine 133
... proliferating cell nuclear antigen (PCNA) were obtained from 5 Prime–3 Prime, ...rat stellate cells in primary culture or freshly isolated from rat livers was performed by Western blot following the ...
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miRNA-338-3p/CDK4 signaling pathway suppressed hepatic stellate cell activation and proliferation
... of proliferation, we identified CDK4, a member of the cyclin-dependent kinase family, as a candidate target ...the cell cycle in G1/S ...for cell proliferation [35, ...and proliferation ...
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Synectin promotes fibrogenesis by regulating PDGFR isoforms through distinct mechanisms
... studying hepatic stellate cell (HSC), since they are key regulators of liver fibrosis ...migration, proliferation, and release of extracellular matrix pro- ...
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Mitochondrial uncouplers inhibit hepatic stellate cell activation
... human hepatic stellate cells Animals were used in accordance with institutional eth- ical ...the cell suspension ...and proliferation analyzes, ...
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Leucine-rich Repeats and Immunoglobulin 1 (LRIG1) Ameliorates Liver Fibrosis and Hepatic Stellate Cell Activation via Inhibiting Sphingosine Kinase 1 (SphK1)/Sphingosine-1-Phosphate (S1P) Pathway
... present study showed that LRIG1 promoted the proliferation rate of LX-2 cells as early as 72 h after TGF-β-induced. The caspase3 activity assay revealed that the apoptosis of LX-2 cells treated with TGF-β was ...
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Secreted phospholipase A2 of Clonorchis sinensis activates hepatic stellate cells through a pathway involving JNK signalling
... clonorchiasis. Cell proliferation was detected by methyl thiazolyltetrazolium (MTT) assay when LX-2 cells were incubated with 50 μg/ml of CsESPs ...of hepatic fibrosis ...cause cell death for ...
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