hepatitis B virus X protein
Hepatitis B Virus X Protein Interferes with Cellular DNA Repair
... The hepatitis B virus X protein (HBx) is a broadly acting transactivator implicated in the development of liver ...tumor-associated virus might contribute to ...
7
Upregulation of MicroRNA 146a by Hepatitis B Virus X Protein Contributes to Hepatitis Development by Downregulating Complement Factor H
... in hepatitis B virus (HBV) patients are caused by immune responses of the ...HBV X protein (HBx) was responsible for its effects on miR-146a expression through NF-B-mediated ...
11
Proteasome complex as a potential cellular target of hepatitis B virus X protein.
... Cross-hybridization demonstrated that some of them were derived from the same cDNA, resulting in a total of four groups (one group of four was of the XAPC7 clone). These clones were retransfected into EGY48 with HBX and ...
10
Naringenin attenuates hepatitis B virus X protein induced hepatic steatosis
... fluorescent protein (GFP) fu- sion protein (HBx-GFP), were maintained in complete Minimal Essential Medium (MEM) supplemented with 100 μg/mL G418 and 50 μg/mL ...
10
Hepatitis B virus X protein is not central to the viral life cycle in vitro.
... Naturally occurring missense mutation in the Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest To exclude a pX-like contribution of HuH-7 hepatoma cells to viral gene exp[r] ...
5
Turnover of Hepatitis B Virus X Protein Is Regulated by Damaged DNA-Binding Complex
... of X protein with the proteasome pathway. Rapid turnover of the X protein has previously been shown to reflect its targeting to proteasome-mediated degra- dation through ubiquitination ...
7
Hepatitis B virus X protein represses miRNA 148a to enhance tumorigenesis
... ChIP. ChIP assay was performed with LO2 cells transfected with HBx or empty vector using the Magna ChIP Assay Kit (Millipore) according to the manufacturer’s instructions (58). Protein-DNA complexes were pre- ...
17
Spatiotemporal Analysis of Hepatitis B Virus X Protein in Primary Human Hepatocytes
... that the nuclear accumulation of HBx depends on interaction with a cellular protein (27, 28). This hypothesis would explain why the capacity for nuclear import or retention of HBx is limited. Notably, the ...
16
Hepatitis B Virus X Protein Is both a Substrate and a Potential Inhibitor of the Proteasome Complex
... (PSMA7; initially referred to as XAPC7) and demonstrated that this interaction is functionally important for the transac- tivation function of HBX (22). This interaction has also been independently reported by two other ...
10
Hepatitis B virus X protein interacts with β5 subunit of heterotrimeric guanine nucleotide binding protein
... Y187 yeast strain was transformed individually with each of the 3 control bait constructs empty pGBKT7 vector, full length HBX in pGBKT7 vector and negative pGBKT7- Lamin control vector. Colonies that grew on SD/-Trp ...
8
The Hepatitis B Virus X Protein Transactivates Viral Core Gene Expression In Vivo
... the X gene colocalizes with these sequences, the cex construct carried a functional X ...Px, X promoter; HBx, X-ORF; p(A), polyadenylation ...the X gene (indicated by the vertical ...
7
Hepatitis B Virus X Protein Function Requires Zinc Binding
... Triton X-100 (TX-100), and ...buffer B (25 mM Tris [pH ...uncleaved protein, and the tag-removed fractions were pooled and further purified by size exclusion chromatography using a 120-ml Superdex 200 ...
18
Hepatitis B virus X protein interacts with a probable cellular DNA repair protein.
... of X, containing only amino acids 1 to 67, did not interact with XAP-1 in the yeast transformation and immunoprecipitation ...the X protein in the target protein ...the X ...
8
Mapping of B-cell epitopes of the human hepatitis B virus X protein.
... The carboxy-terminal half of the HBx antigen was the most frequent target for anti-HBx antibodies in sera of patients with viral hepatitis and contained an immunodominant antigenic regio[r] ...
8
Hepatitis B virus x protein induces epithelial mesenchymal transition of hepatocellular carcinoma cells by regulating long non coding RNA
... Fig. 3 ZEB2-AS1 was an important lncRNA that mediated HBx-induced EMT. a The level of ZEB2-AS1 was verified by a real-time PCR assay. The siRNA can effectively inhibit the level of ZEB2-AS1. b Transwell assay. ...
9
trans-activation of viral enhancers by the hepatitis B virus X protein.
... b X protein for trans-activating the HBV enhancer was derived from pSVX by co-transfecting this plasmid with pHBE7 into PLC/PRF/5 cells, and pEN4 provided the X protein for the trans-act[r] ...
8
Hepatitis B virus X protein activates transcription factor NF-kappa B without a requirement for protein kinase C.
... addition, TPA did not further stimulate CAT activity in cells cotransfected with pwtX and pKB-CAT data not shown, demonstrating the efficient activation of sequestered NF-KB by X protein[r] ...
9
An update on the LIM and SH3 domain protein 1 (LASP1): a versatile structural, signaling, and biomarker protein
... with hepatitis B surface antigen and serum alpha-fetoprotein level of HCC patients [77], suggesting a link between LASP1 and tumorigenesis upon viral ...the hepatitis B virus X ...
17
In Silico Prediction and Docking of Tertiary Structure of Multifunctional Protein X of Hepatitis B Virus
... Through molecular dynamic simulation, whole structure relaxation was done (23, 24). The refined models were assessed by several validation tools to select the best model and assess the quality of that model. National ...
12
Enhancement of Hepatitis B Virus Replication by the Regulatory X Protein In Vitro and In Vivo
... in virus replication within the context of acute hepatitis in a small animal ...in virus replication, including intrahepatic core protein and capsid- associated ...core protein, and ...
7