hepatitis B X protein
Upregulation of MicroRNA 146a by Hepatitis B Virus X Protein Contributes to Hepatitis Development by Downregulating Complement Factor H
... in hepatitis B virus (HBV) patients are caused by immune responses of the ...HBV X protein (HBx) was responsible for its effects on miR-146a expression through NF-B-mediated ...
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Turnover of Hepatitis B Virus X Protein Is Regulated by Damaged DNA-Binding Complex
... Plasmid constructs. The WHx-expressing plasmids have been described pre- viously (43). The HBx expression plasmid was created by ligating the BglII insert (HA-X gene) from the yeast pACT2-HBx vector (44) into the ...
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Hepatitis B Virus X Protein Is both a Substrate and a Potential Inhibitor of the Proteasome Complex
... The hepatitis B virus X protein (HBX) is essential for the establishment of HBV infection in vivo and exerts a pleiotropic effect on diverse cellular ...of protein breakdown by protea- ...
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The Hepatitis B Virus X Protein Transactivates Viral Core Gene Expression In Vivo
... the X gene colocalizes with these sequences, the cex construct carried a functional X ...Px, X promoter; HBx, X-ORF; p(A), polyadenylation ...the X gene (indicated by the vertical ...
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In Silico Prediction and Docking of Tertiary Structure of Multifunctional Protein X of Hepatitis B Virus
... Through molecular dynamic simulation, whole structure relaxation was done (23, 24). The refined models were assessed by several validation tools to select the best model and assess the quality of that model. National ...
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Hepatitis B virus X protein interacts with β5 subunit of heterotrimeric guanine nucleotide binding protein
... the X- α -Galactosidase assay is a reliable method for determin- ing positive interactions in the yeast-two-hybrid ...fusion protein and the BD fusion protein in the two separate yeast ...
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Hepatitis B virus X protein activates transcription factor NF-kappa B without a requirement for protein kinase C.
... addition, TPA did not further stimulate CAT activity in cells cotransfected with pwtX and pKB-CAT data not shown, demonstrating the efficient activation of sequestered NF-KB by X protein[r] ...
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Naringenin attenuates hepatitis B virus X protein induced hepatic steatosis
... fluorescent protein (GFP) fu- sion protein (HBx-GFP), were maintained in complete Minimal Essential Medium (MEM) supplemented with 100 μg/mL G418 and 50 μg/mL ...
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Proteasome complex as a potential cellular target of hepatitis B virus X protein.
... Cross-hybridization demonstrated that some of them were derived from the same cDNA, resulting in a total of four groups (one group of four was of the XAPC7 clone). These clones were retransfected into EGY48 with HBX and ...
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Hepatitis B virus whole-X and X protein play distinct roles in HBV-related hepatocellular carcinoma progression
... Cell cycle and apoptosis were observed using SK-Hep-1 cell lines stably transfected with pCMV-Tag2A-wX, pCMV-Tag2A-X and control plasmids by flow cytometry analysis (FCM). Cells were harvested (1-2 × 10 6 ...
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DDB2 Induces Nuclear Accumulation of the Hepatitis B Virus X Protein Independently of Binding to DDB1
... replication. Moreover, levels of DDB1 and DDB2 fell signifi- cantly during the S phase, suggesting that the nuclear levels of DDB1 and DDB2 are tightly regulated in the liver. If DDB1 and DDB2 are indeed physiological ...
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The Hepatitis B Virus X Protein Modulates Hepatocyte Proliferation Pathways To Stimulate Viral Replication
... the hepatitis B virus (HBV); HBV is the prototype member of the Hepadna- viridae, a family of hepatotropic viruses ...and X (HBx) proteins ...
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An update on the LIM and SH3 domain protein 1 (LASP1): a versatile structural, signaling, and biomarker protein
... with hepatitis B surface antigen and serum alpha-fetoprotein level of HCC patients [77], suggesting a link between LASP1 and tumorigenesis upon viral ...the hepatitis B virus X ...
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The Enigmatic X Gene of Hepatitis B Virus
... tor of activated T cells, NF-AT (59). Activation of this protein is strongly regulated by cytosolic calcium signaling, another known regulator of Src kinases (reviewed in reference 95). This finding led to studies ...
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Hepatitis B Virus X Protein Activates the p38 Mitogen-Activated Protein Kinase Pathway in Dedifferentiated Hepatocytes
... (10, 32). Considering the effect of pX expression on hepatocyte physiology, pX-mediated c-Src activation is also central in the activation of the mitogenic Ras–Raf–mitogen-activated pro- tein kinase (MAPK) (7, 23) and ...
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Molecular characterization of hepatitis B virus X gene in chronic hepatitis B patients
... Hepatitis B virus (HBV) belongs to the Hepadnaviridae family and can be classified into eight genotypes (A to ...and X (HBV-X) proteins [1,2]. HBV-X is a 154 aminoacid multifunctional ...
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Hepatitis B virus X protein is not central to the viral life cycle in vitro.
... Naturally occurring missense mutation in the Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest To exclude a pX-like contribution of HuH-7 hepatoma cells to viral gene exp[r] ...
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Hepatitis B Virus X Protein Function Requires Zinc Binding
... Triton X-100 (TX-100), and ...buffer B (25 mM Tris [pH ...uncleaved protein, and the tag-removed fractions were pooled and further purified by size exclusion chromatography using a 120-ml Superdex 200 ...
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trans-activation of viral enhancers by the hepatitis B virus X protein.
... b X protein for trans-activating the HBV enhancer was derived from pSVX by co-transfecting this plasmid with pHBE7 into PLC/PRF/5 cells, and pEN4 provided the X protein for the trans-act[r] ...
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Hepatitis B virus transactivator X protein is not tumorigenic in transgenic mice.
... Although the expression of mouse AAT protein in the transgenic mice remained high throughout the life span of the animals 43, the pattern of transgene expression in the ATX transgenic mi[r] ...
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