Heterologous Expression
Natural products from filamentous fungi and production by heterologous expression
... through heterologous expression (Lazarus et ...host. Heterologous expression is consid- ered to be the best available option when pursuing character- isation of biosynthetic genes in fungi ...
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Challenges and advances in the heterologous expression of cellulolytic enzymes: a review
... viable expression of indi- vidual cellulases in yeast systems (Table ...their expression in separate single-cell systems ...level expression and production than others ...the expression of ...
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Heterologous expression of a Streptomyces cyaneus laccase for biomass modification applications
... cultures, heterologous systems face challenges such as codon usage bias, the need for signal peptides, protein accumu- lation as inclusion bodies, the absence of necessary post- translational modifications, and ...
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Heterologous expression and site directed mutagenesis of soluble methane monoxygenase
... The purpose of this investigation was to study the heterologous expression of soluble methane monooxygenase sMMO genes from Methylococcus capsula/us Bath and Methylosinus trichosporium O[r] ...
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Heterologous expression of DsRed2 in young sponges (Porifera)
... proved heterologous expression in functional sponges to be possible, we consider this organism as ready for functional ...for heterologous expression in Hydra vulgaris were reported‘(Boettger ...
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Heterologous expression of Gaeumannomyces graminis lipoxygenase in Aspergillus nidulans
... Heterologous expression of G. graminis LOX in A. nidulans was performed according to previous studies (Nyyssölä et al. 2012). The gene encoding the G. graminis LOX AAK81882.1 was codon-optimized for ...
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Heterologous expression of abaecin peptide from Apis mellifera in Pichia pastoris
... For heterologous expression of abaecin in Pichia pastoris, we designed an ORF with HisTag, and the codon usage was ...pPIC9 expression vector and transformed into ...
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Designing, docking and heterologous expression of an anti-HER2 affibody molecule
... currently used for molecular imaging and various detections [2, 19, 20]. The small sizes (4-15 kDa) of these tracers were found to enable efficient imaging contrast and tissue specific molecular detection [11]. ...
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Heterologous expression of plasmodial proteins for structural studies and functional annotation
... host expression system can be optimized to ensure expression plasmid stability, the absence of harm- ful natural proteases and availability of the relevant genetic elements, ...the expression and ...
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Chromosomal position effect influences the heterologous expression of genes and biosynthetic gene clusters in Streptomyces albus J1074
... was generated as described previously [14]. Eighteen mutants from this library with the randomly distributed reporter gene were isolated and analysed. The insertions were mapped on the chromosome using rescue cloning, as ...
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Heterologous expression, purification and enzyme activity of Neocallimastix sp. CellulaseA (celA)
... Neocallimastix sp was obtained as a kind gift from Department of Animal Science, Faculty of Agriculture, Kahramanmaras Sutcu Imam University, Kahramanmaras, Turkey. The following expression hosts were used in this ...
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Heterologous expression of Chlamydia trachomatis polymorphic membrane proteins for in vitro studies
... for expression screening as the insert within the vectors was incorrect although further analysis has shown an error within the reverse primer used to amplify the pmpD passenger domain (primer set: pmpD-N) was ...
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Heterologous expression of antigenic peptides in Bacillus subtilis biofilms
... of expression of a TasA fusion protein, we first monitored the abundance of TasA-mCherry, engi- neered as a tapA-sipW-tasA-mCherry operon in differ- ent genetic ...the expression of this operon in both the ...
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Insights on Heterologous Expression of Fungal Cellulases in Pichia pastoris
... integrated expression cassettes enhance the quantity of produced enzymes in a linear manner ...integrated expression cassettes using quantitative real time PCR (qRT-PCR) in ...
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Heterologous expression of glucose oxidase in the yeast Kluyveromyces marxianus
... The constructions using INU1 cassette for GOX expression started with amplification of the INU1 cas- sette from the chromosomal DNA of K. marxianus CBS 6556 using primers PINU1Fb and PINU1Rb (Table 5). Both ...
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HKT transporters : state of the art
... using heterologous expression of OsHKT2;1 in yeast cells and Xenopus oocytes revealed that OsHKT2;1 mediates both inward and outward Na + currents ...
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Enhanced production of recombinant serratiopeptidase in Escherichia coli and its characterization as a potential biosimilar to native biotherapeutic counterpart
... approaches. Heterologous expression of serratiopeptidase in ...other expression related issues due to its inherent proteolytic activity such as cytotoxicity, cell death, no expression, minimal ...
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Expansion of the TLO gene family enhances the virulence of Candida species
... In the second part of our study, to better understand the diversity of functions regulated by TLOs, we expressed a range of C. albicans TLO genes in a ΔΔtlo C. dubliniensis background. In general, heterologous ...
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Broad Host Range Expression Reveals Native and Host Regulatory Elements That Influence Heterologous Antibiotic Production in Gram Negative Bacteria
... Heterologous expression has become a powerful tool for the investigation of microbial BGCs for several ...Second, heterologous expression of a defined set of genes allows for direct connection ...
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Recombinant production of bacterial toxins and their derivatives in the methylotrophic yeast Pichia pastoris
... ual selection largely favoured the most-preferred P. pas- toris codons, but in certain instances the second-most preferred codons were selected instead to ensure an over- all reduction in the A+T-content of the resulting ...
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