heterologous protein
Establishment of Neurospora crassa as a host for heterologous protein production using a human antibody fragment as a model product
... Despite their positive features, filamentous fungi also pose specific challenges in their use as microbial cell factories. The saprophytic fungal life style involves the secretion of catabolic enzymes into the growth ...
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A New Biophysical Model for Translation Elongation to Predict and Optimize Heterologous Protein Production in Escherichia coli.
... Ribosome displacement is the misalignment between the A-site and the normal “zero” reading frame as defined by the start codon. Misalignment is due to secondary ribosome movements (whereas translocation is the primary ...
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Enhancing co-translational folding of heterologous protein by deleting non-essential ribosomal proteins in Pichia pastoris
... accumulate heterologous proteins in a time-dependent manner, the relative mRNA level of the Phy remained constant over the time and across the strains, with a standard deviation of only 50 parts per million (ppm), ...
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Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: A review
... binant protein produced in Pichia seems to be decided largely by its inherent properties such as amino acid sequence, the tertiary structure and the site of expression ...the heterologous product ...the ...
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Enhanced heterologous protein productivity by genome reduction in Lactococcus lactis NZ9000
... in heterologous protein production and secretion, have been analyzed in ...of heterologous proteins ...of heterologous proteins ...of heterologous protein was reduced, but also ...
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Development of an antibiotic marker-free platform for heterologous protein production in Streptomyces
... plasmid, and ultimately used for heterologous protein production. Thus, the antitoxin counteracts the effect of the toxin when the expression plasmid is present in the cell. By contrast, when the plasmid is ...
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Combinatorial Sec pathway analysis for improved heterologous protein secretion in Bacillus subtilis: identification of bottlenecks by systematic gene overexpression
... With construction of the 23 recombinant strains above, the effect of single gene involved in Sec pathway on the production and secretion of heterologous protein was studied systematically. The expression ...
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Use of folding modulators to improve heterologous protein production in Escherichia coli
... of heterologous protein folding in the organism, due largely to their broad substrate range compared with, ...e.g., protein-specific mutagenesis ...of heterologous proteins in the bacterium ...
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Design of an efficient medium for heterologous protein production in Yarrowia lipolytica: case of human interferon alpha 2b
... defined composition, a high batch-to-batch variability and a high cost. On the other hand, the majority of publications in the field of recombinant proteins pro- duction by Y. lipolytica report the use of the constitu- ...
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Transcriptomic and fluxomic changes in Streptomyces lividans producing heterologous protein
... erologous protein production in other micro-organisms, ...augments heterologous protein production ...for protein production, in this case including heterologous CelA protein as ...
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Protein secretion in Lactococcus lactis: an efficient way to increase the overall heterologous protein production
... total protein detected as mature form in the supernatant) resulted in increased overall amounts of the ...i) heterologous proteins pro- duced in ...than protein size is the predominant feature that ...
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Optimizing heterologous protein production in the periplasm of E. coli by regulating gene expression levels
... functional protein in the periplasm To address if the optimization of the production of processed BL1 results in properly folded protein, we ex- amined the binding ability to its substrate, β- galactosidase ...
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An encoded N-terminal extension results in low levels of heterologous protein production in Escherichia coli
... low protein levels of long-versus short-form ...Tdk protein is present at a low level with such induction ...in protein levels between these two forms is not due to differential ...
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Targeting a heterologous protein to multiple plant organelles via rationally designed 5′ mRNA tags
... Subcellular localization of TriTag-1 and TriTag-2 fused GFP TriTag-1 and TriTag-2 were designed to target the re- sultant protein to either the chloroplast or peroxisome via alternative splicing of the encoding ...
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Imbalance of heterologous protein folding and disulfide bond formation rates yields runaway oxidative stress
... high protein degradation rates, repressed overall expres- sion, and oxidative stress in response to +/- UPR strains secreting different sized recombinant ...engineering protein secretion and implies the ...
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Heterologous protein display on the cell surface of lactic acid bacteria mediated by the s-layer protein
... To evaluate the possibility of the LcsB region as a cell wall anchor, the LcsB region was tagged with green fluorescent protein (GFP), a convenient tool to investigate the protein localization in cells ...
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The Clearance of Heterologous Protein from the Circulation of Normal and Immunized Man
... Intravenously injected streptokinase was cordingly streptokinase antibody assays were performed cleared from the circulation at two distinct rates, upon the isolated gamma globulin fract[r] ...
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A structurally informed autotransporter platform for efficient heterologous protein secretion and display
... N-terminal fusion of ESAT6 to an Hbp passenger that was truncated down to residue 557 (HbpTr557), just downstream of side domain d2, resulted in a ~2-fold de- crease in the recovery of cleaved Hbp-ESAT6 passenger ...
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Functional Heterologous Protein Expression by Genetically Engineered Probiotic Yeast Saccharomyces boulardii
... recombinant protein even when recovered from gastrointestinal immune tissues in ...recombinant protein and are resistant in vitro to low pH, bile acid salts, and anaerobic ...recombinant protein ...
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Translation of bovine leukemia virus virion RNAs in heterologous protein-synthesizing systems.
... laevis oocytes resulted in the synthesis of small amounts of a 145,000-molecular weight polypeptide P145 that was immunoprecipitated by an anti-p24 serum and not by an anti-gp6O serum an[r] ...
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